Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 216-305-9 | CAS number: 1555-58-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Range-Finding Toxicity data List V
- Author:
- Smyth Jr H.F., Carpenter C.P., Weil C.S., Pozzani U.C.
- Year:
- 1 954
- Bibliographic source:
- AMA Arch. Ind. Hyg. Occup. Med. 10, 61-68 (1954)
- Reference Type:
- publication
- Title:
- Chemical burns of the rabbit cornea
- Author:
- Carpenter C.P., Smyth H.F.
- Year:
- 1 946
- Bibliographic source:
- American Journal of Ophthalmology, Vol. 29, Issue 11, p1363–1372
Materials and methods
- Principles of method if other than guideline:
- The potential of the test substance to cause eye injury was assessed. The degree of corneal necrosis from various volumes and concentrations of the chemical was reported.
- GLP compliance:
- no
Test material
- Reference substance name:
- 3,3'-(methylimino)bispropiononitrile
- EC Number:
- 216-305-9
- EC Name:
- 3,3'-(methylimino)bispropiononitrile
- Cas Number:
- 1555-58-4
- Molecular formula:
- C7H11N3
- IUPAC Name:
- 3-[(2-cyanoethyl)(methyl)amino]propanenitrile
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- - Name as used in publication: N-Methyl-3,3'-iminodipropionitrile
Test animals / tissue source
- Species:
- rabbit
- Strain:
- other: normal albino rabbits, not further specified.
- Details on test animals or tissues and environmental conditions:
- Rabbits were selected on the bases of absence of grossly visible staining by a 5% aqueous solution of fluorescein sodium, flushed with distilled water 20 seconds after application.
Test system
- Vehicle:
- other: undiluted
- Controls:
- not specified
- Amount / concentration applied:
- Depending on effects ranging between 0.5 mL undiluted to 0.005 mL of a 1% solution in water of propylene glycol.
- Duration of treatment / exposure:
- Substance was not washed out
- Observation period (in vivo):
- 18-24 hours
- Number of animals or in vitro replicates:
- Usually 5 eyes were exposed
- Details on study design:
- The eye injury test in rabbits reports the degree of corneal necrosis from various volumes and concentrations of chemical. Different amounts of the undiluted or dilured test material is apllied to the center of the cornea while the lids are retracted. About 1 minute later, the lids were released. The exposed eyes were were examined in strong daylight., then stained with fluorescein, and the injury scored.Eye injury was translated into a numerical score. The individual numerical scored of each eye treated with a given volume or concentration of a chemical were added together and then divided by the number of eyes to obtain the score of the injury caused by the treatment. A score of 5.0 was representative of severe injury, corresponding to necrosis, visible only after staining and covering about three fourth of the surface of the cornea; or a more severe necrosis covering a smaller area.System for numerical scoring of injury to the rabbit eye 24 hours after apllication:- Symptom visible before fluorescein staining-- Cornea dull: 2 points-- Cornea opaque, less than half of area: 4 points-- Cornea opaque, more than half of area: 6 points-- Iritis, slight internal congestion: 1 point-- Iritis, marked internal congestion: 2 points-Symptom visible after fluorescein staining (If necrosis is diffuse assign points corresponding to half observed area)-- Necrosis on less than 5% of cornea: 1 point-- Necrosis on 5 to 12 % 2 points-- Necrosis on 13 to 37 % 3 points-- Necrosis on 38 to 62 % 4 points-- Necrosis on 63 to 87 % 5 points-- Necrosis on 88 to 100 % 6 pointsGrades of injury employed for rating the relative damage produced by chemicals in the eye:1: 0.5 mL undiluted gives injury of 0 to 1.0 points2: 0.5 mL undiluted gives injury of 1.0 to 5.0 points3: 0.1 mL undiluted gives injury of up to 5.0 points (0.5 mL gives over 5.0)4: 0.02 mL undiluted gives injury of up to 5.0 points (0.1 mL gives over 5.0)5: 0.005 mL undiluted gives injury of up to 5.0 points (0.02 mL gives over 5.0)6: Excess of 40 % solution gives injury of up to 5.0 point (0.005 mL gives over 5.0)7: Excess of 15 % solution gives injury of up to 5.0 point (40% gives over 5.0)8: Excess of 5 % solution gives injury of up to 5.0 point (15% gives over 5.0)9: Excess of 1 % solution gives injury of up to 5.0 point (5% gives over 5.0)10: Excess of 1 % solution gives injury of over 5.0 point.
Results and discussion
In vivo
Results
- Irritation parameter:
- other: Grade
- Basis:
- mean
- Time point:
- other: 18 to 24 hours
- Score:
- 2
- Max. score:
- 10
- Reversibility:
- not specified
- Remarks on result:
- other: 0.5 mL undiluted test substance gives injury of over 1.0 up to 5 points
- Irritant / corrosive response data:
- Based on the applied system for numerical scoring of injury to the rabbit eye 24 hours after apllication, 0.5 mL of the undiluted test substance gave a score between 1 and 5.
Applicant's summary and conclusion
- Interpretation of results:
- Category 2A (irritating to eyes) based on GHS criteria
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.