Trisodium 5-[[4-chloro-6-(ethylphenylamino)-1,3,5-triazin-2-yl]amino]-4-hydroxy-3-[(1-sulphonato-2-naphthyl)azo]naphthalene-2,7-disulphonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1984

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Justification for type of information:

None

Qualifier:

according to guideline

Guideline:

other: Internal method " Method A-11, Acute Fish Toxicity"

Version / remarks:

Equivalent to OECD Guideline 203

Deviations:

no

Principles of method if other than guideline:

None

GLP compliance:

no

Specific details on test material used for the study:

FAT 40147/E

Analytical monitoring:

no

Details on sampling:

None

Vehicle:

not specified

Details on test solutions:

None

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Zebra fish

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

None

Test temperature:

23 °C

pH:

8

Dissolved oxygen:

7.6 - 7.7 mg/L

Salinity:

None

Conductivity:

None

Nominal and measured concentrations:

300 and 1000 mg/L

Details on test conditions:

- No of fish tested for each concentration: 10 each
Refer " Any other information on material and methods incl. tables" for details

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

620 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No mortality was observed with the concentration 300 mg/L throughout the observation period. However, 10 out of 10 fish were found to be dead at the 96 hour observation.

Results with reference substance (positive control):

None

Reported statistics and error estimates:

None

Sublethal observations / clinical signs:

None

Validity criteria fulfilled:

not specified

Conclusions:

LC50 was determined to be 620 mg/L.

Executive summary:

The acute fish toxicity of FAT 40147/E was assessed in a study conducted according to the internal Ciba method "Method A-11, Acute Fish toxicity", which is equivalent to OECD Guideline 203. Two groups of 10 zebra fish each, were exposed to test concentrations of 300 and 1000 mg/L for 96 hours. No mortality was observed with the concentration 300 mg/L throughout the observation period. However, 10 out of 10 fish were found to be dead with the concentration 1000 mg/L at the 96 hour observation. Hence, the LC50 was determined to be 620 mg/L.

Description of key information

FAT 40147 was determined to have a 96 hour LC50 value of 620 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

620 mg/L

Additional information

The short term toxicity potential of the substance to fish was assessed using four available studies with zebra fish.

In a key study, acute fish toxicity of FAT 40147/E was assessed according to the internal Ciba method "Method A-11, Acute Fish toxicity", which is equivalent to OECD Guideline 203. Two groups of 10 zebra fish each, were exposed to test concentrations of 300 and 1000 mg/L for 96 hours. No mortality was observed with the concentration 300 mg/L throughout the observation period. However, 10 out of 10 fish were found to be dead with the concentration 1000 mg/L at the 96 hour observation. Hence, the LC50 was determined to be 620 mg/L.

In other three remaining supporting studies conducted on different batches of the test material, the 96 hour LC50 for FAT 40147 was determined to be >1000 mg/L.

Hence, the lowest value for 96 hour LC50 can be taken forward for hazard assessment to fish.