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EC number: 204-255-0 | CAS number: 118-48-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: other routes
Administrative data
- Endpoint:
- acute toxicity: other routes
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 14.-25.1.1971
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Basic data are given.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 971
- Report date:
- 1971
Materials and methods
- Principles of method if other than guideline:
- BASF-Test
- GLP compliance:
- no
- Remarks:
- pre-GLP study
- Limit test:
- no
Test material
- Reference substance name:
- 4H-3,1-benzoxazine-2,4(1H)-dione
- EC Number:
- 204-255-0
- EC Name:
- 4H-3,1-benzoxazine-2,4(1H)-dione
- Cas Number:
- 118-48-9
- Molecular formula:
- C8H5NO3
- IUPAC Name:
- 4H-3,1-benzoxazine-2,4(1H)-dione
- Details on test material:
- Isatosäureanhydrid (Isatoic anhydride); no further data
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- other: Kisslegg
- Sex:
- male/female
Administration / exposure
- Route of administration:
- intraperitoneal
- Vehicle:
- other: aqueous Tylose
- Details on exposure:
- VEHICLE
- Concentration in vehicle: 2, 4, 8, 10, 16%
- Amount of vehicle (if gavage): 10 ml/kg bw
According to the authors, the test substance is hydrolysed by water.
MAXIMUM DOSE VOLUME APPLIED: 10 ml/kg bw
No further data. - Doses:
- 200, 400, 800, 1000, 1250, 1600 mg/kg
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 10 days
- Frequency of observations and weighing: beginning, mid and end of study
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs - Statistics:
- no data
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- ca. 1 250 mg/kg bw
- Mortality:
- no data
- Clinical signs:
- dyspnea, twitching, convulsive tremor, prone position
- Body weight:
- no data
- Gross pathology:
- peritoneal adhesions, pulmonary plethora, clayish-grey coloration of the liver
Any other information on results incl. tables
Dose mg/kg |
Conc. % |
Animal |
Dead after 1 h |
24 h |
48 h |
7 days |
1600 |
16 |
5 m 5 f |
0/5 0/5 |
5/5 5/5 |
5/5 5/5 |
5/5 5/5 |
1200 |
10 |
5 m 5 f |
0/5 0/5 |
3/5 1/5 |
3/5 1/5 |
3/5 1/5 |
1000 |
10 |
5 m 5 f |
0/5 0/5 |
1/5 0/5 |
1/5 0/5 |
1/5 0/5 |
800 |
8 |
5 m 5 f |
0/5 0/5 |
0/5 0/5 |
0/5 0/5 |
0/5 0/5 |
400 |
4 |
5 m 5 f |
0/5 0/5 |
0/5 0/5 |
0/5 0/5 |
0/5 0/5 |
200 |
2 |
5 m 5 f |
0/5 0/5 |
0/5 0/5 |
0/5 0/5 |
0/5 0/5 |
1600 mg/kg: 5 min after injection tremors, morphin tails, aequous secretion from the oral cavity, dyspnea. The animals died within 1.75 h.
1250-1000 mg/kg: Dyspnea right after injection, mock attacks and cry of pain. 3.5 h later survivors showed abdominal position, dyspnea and light apathy. From day 2-4 they still showed dyspnea but no symptoms from day 5 on.
800-400 mg/kg: Agitating right after injection, irregular gait, cavernous flanks, dyspnea and convulsions. From 5 hours till 2 days after injection they showed abdominal position, dyspnea and semi-closed eyes. From day 4 on they were without symptoms.
Section: died animals: blood, coloration and degeneration of the liver; sacrificed animals: blunt liver borders, peritoneal adhesions
Applicant's summary and conclusion
- Executive summary:
LD50 was ca. 1250 mg/kg bw after 7 days
Groups of mice were administered a preparation of the test substance in aqueous Tylose and were observed for 7 days. The test substance was found to hydrolyse in water. Clinical signs of toxicity comprised dyspnea, twitching, convulsive tremor, and prone position. Pathology revealed peritoneal adhesions, pulmonary plethora, and clayish-grey coloration of the liver.
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