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EC number: 206-376-4 | CAS number: 334-48-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study with acceptable restrictions
Data source
Referenceopen allclose all
- Reference Type:
- other: NTP database entry
- Title:
- Unnamed
- Year:
- 1 985
- Reference Type:
- publication
- Title:
- Salmonella Mutagenicity Tests. IV. Results from the Testing of 300 Chemicals
- Author:
- Zeiger, E. et al.
- Year:
- 1 988
- Bibliographic source:
- Environ Molec Mutagen 11(12):1-158
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- strain with AT base pair as primary reversion site missing
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Decanoic acid
- EC Number:
- 206-376-4
- EC Name:
- Decanoic acid
- Cas Number:
- 334-48-5
- Molecular formula:
- C10H20O2
- IUPAC Name:
- decanoic acid
Constituent 1
Method
- Target gene:
- his operon
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- S. typhimurium TA 97
- Metabolic activation:
- with and without
- Metabolic activation system:
- induced male Sprague Dawley rat liver S9 (10% and 30% S9 in S9-mix) and induced male Syrian hamster liver S9 (10% and 30% S9 in S9-mix)
- Test concentrations with justification for top dose:
- 10, 33, 100, 333 and 666 µg/plate with and without metabolic activation
3, 10, 33, 100, 333 and 666 µg/plate without metabolic activation - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- no
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-nitrofluorene or alternatively 4-nitro-o-phenylenediamine (-S9, TA98); sodium azide (-S9, TA100 and TA1535); 9-aminoacridine (-S9, TA97 and TA1537); 2-aminoanthracene (+S9, all strains)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: preincubation
DURATION
- Preincubation period: 20 min
- Exposure duration: 48 hours - Evaluation criteria:
- If the test chemical was mutagenic to any particular strain of bacterium, the number of histidine-independent colonies arising on those plates will be significantly greater than the corresponding control plates for that strain.
The number of mutant colonies appearing on the positive control plates must be significantly increased over the spontaneous control number for the test to be considered valid. Failure of the positive control chemical to induce mutation is reason to discard the experiment.
If no increase in mutant colonies is seen after testing several strains under several different culture conditions, the test chemical is considered to be nonmutagenic in the Ames test. - Statistics:
- Mean values and standard deviation were calculated.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- slight toxicity for all strains tested at 666 µg/plate without S9 and for TA 1535 at 666 µg/plate with 10% rat liver S9
- Vehicle controls validity:
- not examined
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 97
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- slight toxicity at 666 µg/plate without S9-mix
- Vehicle controls validity:
- not examined
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
Any other information on results incl. tables
Table 1. Test results for decanoic acid
With or without S9-Mix |
Test substance concentration (μg/plate) |
Mean number of revertant colonies per plate ± standard deviation |
||||
Base-pair substitution type |
Frameshift type |
|||||
TA100 |
TA1535 |
TA97 |
TA98 |
TA1537 |
||
– |
0 |
145 ± 10.5 |
13 ± 2 |
138 ± 6.6 |
17 ± 0.3 |
7 ± 0.7 |
– |
10 |
130 ± 7.2 |
14 ± 1.5 |
150 ± 5.6 |
21 ± 1.5 |
8 ± 0.7 |
– |
33 |
119 ± 15.1 |
15 ± 2.1 |
131 ± 14 |
16 ± 2.6 |
7 ± 0.6 |
– |
100 |
109 ± 6.2 |
11 ± 2.2 |
151 ± 9 |
21 ± 0.6 |
6 ± 0.6 |
– |
333 |
106 ± 4.8 |
14 ± 3.8 |
114 ± 7.5 |
15 ± 1.2 |
6 ± 1 |
– |
666 |
59s ± 2.8 |
7s ± 1.2 |
15s ± 8.1 |
3s ± 2.1 |
2s ± 0.3 |
– |
Positive controls |
SA |
SA |
9AA |
2NF or 4NPD |
9AA |
Mean No. of colonies/plate ± SD |
532 ± 11.9 |
428 ± 21.9 |
793 ± 106.2 |
615 ± 2.3 |
305 ± 22.6 |
|
– |
0 |
157 ± 8.6 |
21 ± 1.2 |
149 ± 11 |
17 ± 2.7 |
7 ± 1.7 |
– |
3 |
119 ± 9.3 |
17 ± 2 |
167 ± 4 |
13 ± 0.9 |
7 ± 0.3 |
– |
10 |
119 ± 3.8 |
11 ± 2.2 |
161 ± 10.1 |
16 ± 1.2 |
7 ± 0.9 |
– |
33 |
106 ± 3.3 |
14 ± 1.5 |
154 ± 2.6 |
18 ± 2 |
9 ± 2.1 |
– |
100 |
103 ± 11 |
17 ± 2.4 |
143 ± 3.6 |
15 ± 2.1 |
5 ± 1.2 |
– |
333 |
135 ± 12.2 |
13 ± 4.2 |
120 ± 12.1 |
18 ± 1.9 |
4 ± 0.9 |
– |
Positive controls |
SA |
SA |
9AA |
2NF or 4NPD |
9AA |
Mean No. of colonies/plate ± SD |
478 ± 8.8 |
444 ± 4.8 |
823 ± 90.6 |
580 ± 22 |
416 ± 43.7 |
|
+ (10% HLI) |
0 |
102 ± 6.3 |
12 ± 2.1 |
173 ± 19.5 |
28 ± 2.6 |
9 ± 2.1 |
+ (10% HLI) |
10 |
104 ± 5.8 |
10 ± 1.7 |
158 ± 10.8 |
28 ± 3.5 |
6 ± 1.9 |
+ (10% HLI) |
33 |
82 ± 2.7 |
12 ± 3.1 |
157 ± 12.8 |
26 ± 5.5 |
7 ± 0.9 |
+ (10% HLI) |
100 |
101 ± 4 |
7 ± 0.9 |
151c ± 15 |
25 ± 4 |
5 ± 1.8 |
+ (10% HLI) |
333 |
92 ± 8 |
6 ± 0.7 |
142 ± 10.1 |
15 ± 2.3 |
5 ± 1.5 |
+ (10% HLI) |
666 |
83 ± 5.9 |
6 ± 1.5 |
108 ± 7.5 |
15 ± 3.2 |
4 ± 2 |
+ (10% HLI) |
Positive controls |
2AA |
2AA |
2AA |
2AA |
2AA |
Mean No. of colonies/plate ± SD |
479 ± 19.2 |
202c ± 9.5 |
386 ± 13.6 |
339 ± 25.9 |
52 ± 1.8 |
|
+ (30% HLI) |
0 |
154 ± 9.7 |
8 ± 1.2 |
179 ± 3.3 |
27 ± 3.2 |
14 ± 1.7 |
+ (30% HLI) |
10 |
134 ± 10.9 |
9 ± 2.5 |
198 ± 5.5 |
28 ± 0.3 |
4 ± 0.6 |
+ (30% HLI) |
33 |
145 ± 19.8 |
9 ± 1.5 |
194 ± 6.8 |
24 ± 2.1 |
6 ± 0.6 |
+ (30% HLI) |
100 |
134 ± 7.9 |
9 ± 0.9 |
194 ± 4.3 |
25 ± 1.8 |
6 ± 0.9 |
+ (30% HLI) |
333 |
142 ± 9.8 |
6 ± 1.2 |
185 ± 6.7 |
24 ± 1.7 |
8 ± 1.5 |
+ (30% HLI) |
666 |
150 ± 5.2 |
6 ± 0.3 |
140 ± 16.3 |
24 ± 4.2 |
5 ± 1 |
+ (30% HLI) |
Positive controls |
2AA |
2AA |
2AA |
2AA |
2AA |
Mean No. of colonies/plate ± SD |
541 ± 13.5 |
459 ± 37.3 |
414 ± 25.2 |
294 ± 3.2 |
47 ± 3.2 |
|
+ (10% RLI) |
0 |
129 ± 11.7 |
9 ± 1.2 |
165 ± 1.3 |
19 ± 2.7 |
10 ± 2.2 |
+ (10% RLI) |
10 |
126 ± 17.5 |
9 ± 1.5 |
189 ± 5.3 |
18 ± 4.6 |
7 ± 1.5 |
+ (10% RLI) |
33 |
138c ± 13 |
8 ± 1.3 |
188 ± 11.6 |
18 ± 4.1 |
8 ± 1.2 |
+ (10% RLI) |
100 |
108 ± 14.8 |
10 ± 1.3 |
122 ± 5.8 |
24 ± 1.5 |
4 ± 0.6 |
+ (10% RLI) |
333 |
87 ± 7.5 |
7 ± 0.3 |
146 ± 1.8 |
15 ± 1.7 |
3 ± 0 |
+ (10% RLI) |
666 |
73 ± 2.9 |
1s ± 0.9 |
57 ± 9 |
12 ± 1.7 |
3 ± 1 |
+ (10% RLI) |
Positive controls |
2AA |
2AA |
2AA |
2AA |
2AA |
Mean No. of colonies/plate ± SD |
390 ± 22.9 |
190 ± 12.5 |
364 ± 5.7 |
201 ± 18.3 |
45 ± 2.6 |
|
+ (30% RLI) |
0 |
142 ± 10.7 |
15 ± 0.7 |
170 ± 15.7 |
27 ± 3.9 |
12 ± 2.3 |
+ (30% RLI) |
10 |
148 ± 15.5 |
19 ± 3.8 |
214 ± 12 |
26 ± 2.7 |
8 ± 2.3 |
+ (30% RLI) |
33 |
139 ± 2.2 |
14 ± 0.6 |
214 ± 13.1 |
22 ± 3.5 |
7 ± 0.6 |
+ (30% RLI) |
100 |
138 ± 20 |
14 ± 0.6 |
187 ± 3.5 |
24 ± 2.6 |
7 ± 0.7 |
+ (30% RLI) |
333 |
132 ± 5.2 |
8 ± 1.2 |
187 ± 3.8 |
17 ± 2.1 |
7 ± 2.1 |
+ (30% RLI) |
666 |
148 ± 8.1 |
10 ± 2.3 |
98 ± 19.5 |
17 ± 3.3 |
4 ± 0.6 |
+ (30% RLI) |
Positive controls |
2AA |
2AA |
2AA |
2AA |
2AA |
Mean No. of colonies/plate ± SD |
787 ± 42.9 |
187 ± 12.5 |
351 ± 7.9 |
159 ± 17.1 |
53 ± 0.6 |
SA = sodium azide
9AA = 9-aminoacridine
4NPD = 4-nitro-o-phenylenediamine
2NF = 2-nitro-fluorene
2AA = 2-aminoanthracene
SD = standard deviation
HLI = induced male Syrian hamster liver S9
RLI = induced male Sprague Dawley rat liver S9
s = slight toxicity
c = contamination
Applicant's summary and conclusion
- Conclusions:
- Under the experimental conditions described, the test substance was not mutagenic in the Ames test using the Salmonella typhimurium strains TA1535, TA1537, TA98, TA100 and TA97 with and without metabolic activation.
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