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EC number: 253-675-0 | CAS number: 37811-72-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Study period:
- 24 - 27 Jan 2002
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study with acceptable restrictions analytical purity of the test substance not specified).
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2002
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- analytical purity of test substance not specified;
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- The Department of Health of the Government of the United Kingdom
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- -
- EC Number:
- 441-620-5
- EC Name:
- -
- Cas Number:
- 163961-32-8
- Molecular formula:
- C20H40O2 (C16iso) C22H44O2 (C18iso) C22H42O2 (C18:1iso)
- IUPAC Name:
- Fatty acids, C16-18 and C18-unsatd., branched and linear, butyl esters
Constituent 1
Method
- Target gene:
- his operon
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- not specified
- Species / strain / cell type:
- S. typhimurium TA 102
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9-Mix
- Test concentrations with justification for top dose:
- Experiment I+II:
- 50, 150, 500, 1500 and 5000 µg/plate (with and without metabolic activation) - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: Acetone
Controlsopen allclose all
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Acetone
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: N -etþl-N'-nitro -N-nitroso guanidine (ENNG, 3µg/plate (TA100), 5µg/plate (TA1535)); 9-Aminoacridine (9AA, 80µg/plate (TA1537)); Mitomycin C (MMC, 0.5µg/plate (TA102); 4-Nitroquinoline- 1 -oxide (4NQO, 0.2 µg/plate (TA98))
- Remarks:
- without S9-mix
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Acetone
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-Aminoanthracene (2-AA, 1µg/plate, TA 100; 2µg/plate, TA 1535 and TA 1537); 1,8 Dihydroxyanthraquinone (DAN, 10µg/plate, TA 102); Benzo(a)pyren (BP, 5µg/plate, TA 98)
- Remarks:
- with S9-mix
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Exposure duration: 37 °C for approximately 48 hours
NUMBER OF REPLICATIONS: triplicates
DETERMINATION OF CYTOTOXICITY
- Method: Reduction of growth of bacterial background lawn
The frequency of revertant colonies was assessed using a Domino colony counter.
- Evaluation criteria:
- The reverse mutation assay may be considered valid if the following criteria are met:
- All tester strain cultures exhibit a characteristic number of spontaneous revertants per plate in the vehicle and untreated controls compared to historical controls.
- The appropriate characteristics for each tester strain have been confirmed.
- All tester strain cultures should be in the approximate range of bacteria per ml.
- Each mean positive control value should be at least two times the respective vehicle control value for each strain.
- There should be a minimum of four non-toxic test material dose levels and no evidence of excessive contamination.
The test material may be considered positive in this test system if the following criteria are met:
- The test material should have induced a reproducible, dose-related and statistically signifrcant increase in the revertant count in at least one strain of
bacteria. - Statistics:
- Mean and standard deviation were calculated. Significance was tested using Dunnett's method of linear regression (Kirkland DJ (Ed) (1989) Statistical Evaluation of Mutagenicity Test Data. UKEMS Subcommittee on Guidelines for Mutagenicity Testing, Report - Part III, Cambridge University Press.)
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity, but tested up to precipitating concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 102
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity, but tested up to precipitating concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
The test material caused no visible reduction in the growth of the bacterial background lawn at any dose level.
The test material was, therefore, tested up to the maximum recommended dose level of 5000 µg/plate.
A globular, oily, opaque precipitate was observed at 5000 µg/plate, this did not prevent the scoring of revertant colonies.
RANGE-FINDING/SCREENING STUDIES:
The test material was non-toxic to the strain of Salmonella used (TA100). The test material formulation and the S9-mix used in this experiment were both shown to be effectively sterile.
COMPARISON WITH HISTORICAL CONTROL DATA:
A history profile of vehicle and positive control values was presented.
ADDITIONAL INFORMATION ON CYTOTOXICITY:
In a preliminary toxicity study the test material was non-toxic to the Salmonella strain TA 100. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Table 1: Results from Experiment 1:
With or without S9-Mix |
Test substance concentration (μg/plate) |
Mean number of revertant colonies per plate (average of 3 plates) |
||||
TA 100 |
TA1535 |
TA102 |
TA98 |
TA1537 |
||
- |
0 |
90 |
20 |
317 |
23 |
18 |
- |
50 |
94 |
19 |
333 |
22 |
17 |
- |
150 |
96 |
20 |
315 |
21 |
14 |
- |
500 |
96 |
24 |
343 |
23 |
19 |
- |
1500 |
94 |
26 |
357 |
27 |
20 |
- |
5000 |
101 |
24 |
349 |
22 |
15 |
Positive controls - S9 |
Name |
ENNG |
ENNG |
MMC |
4NQO |
9AA |
Concentrations (μg/plate) |
3.0 |
5.0 |
0.5 |
02 |
80 |
|
Number of colonies/plate |
439 |
464 |
740 |
96 |
1510 |
|
+ |
0 |
98 |
16 |
380 |
37 |
25 |
+ |
50 |
102 |
15 |
375 |
42 |
25 |
+ |
150 |
101 |
15 |
370 |
40 |
27 |
+ |
500 |
110 |
14 |
355 |
44 |
17 |
+ |
1500 |
104 |
17 |
358 |
44 |
24 |
+ |
5000 |
103 |
15 |
358 |
38 |
30 |
Positive controls + S9 |
Name |
2AA |
2AA |
DAN |
BP |
2AA |
Concentrations (μg/plate) |
1.0 |
2.0 |
10 |
5 |
2.0 |
|
Number of colonies/plate |
2112 |
269 |
06 |
232 |
217 |
Table 2: Results from Experiment 2:
With or without S9-Mix |
Test substance concentration (μg/plate) |
Mean number of revertant colonies per plate (average of 3 plates) |
||||
TA 100 |
TA1535 |
TA102 |
TA98 |
TA1537 |
||
- |
0 |
76 |
21 |
274 |
26 |
11 |
- |
50 |
68 |
23 |
270 |
25 |
8 |
- |
150 |
81 |
24 |
300 |
21 |
9 |
- |
500 |
80 |
25 |
281 |
23 |
10 |
- |
1500 |
85 |
25 |
290 |
24 |
12 |
- |
5000 |
76 |
26 |
280 |
24 |
14 |
Positive controls - S9 |
Name |
ENNG |
ENNG |
MMC |
4NQO |
9AA |
Concentrations (μg/plate) |
3.0 |
5.0 |
0.5 |
02 |
80 |
|
Number of colonies/plate |
679 |
933 |
1167 |
188 |
2357 |
|
+ |
0 |
78 |
14 |
373 |
39 |
19 |
+ |
50 |
77 |
14 |
385 |
46 |
27 |
+ |
150 |
86 |
15 |
401 |
45 |
21 |
+ |
500 |
82 |
14 |
382 |
42 |
19 |
+ |
1500 |
85 |
16 |
400 |
44 |
20 |
+ |
5000 |
91 |
19 |
379 |
39 |
16 |
Positive controls + S9 |
Name |
2AA |
2AA |
DAN |
BP |
2AA |
Concentrations (μg/plate) |
1.0 |
2.0 |
10 |
5 |
2.0 |
|
Number of colonies/plate |
3159 |
313 |
1021 |
312 |
222 |
2AA: 2-Aminoanthracene
BP: Benzo(a)pyrene
DAN: 1,8 Dihydroxyanthraquinone
ENNG: N-ethyl-N'-nitro-N-nitrosoguanidine
MMC: Mitomycin C
4NQO: 4-Nitroquinoline-1-oxide
9AA: 9-Aminoacridine
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
No significant increases in the frequency of revertant colonies were recorded for any of the bacterial strains, with any dose of the test material, either with or without metabolic activation. The test material was considered to be non-mutagenic under the conditions of this test.
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