Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 214-223-8 | CAS number: 1115-30-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 23 February - 9 June 1999
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study according to GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.5 (Acute Toxicity: Eye Irritation / Corrosion)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Diethyl acetylsuccinate
- EC Number:
- 214-223-8
- EC Name:
- Diethyl acetylsuccinate
- Cas Number:
- 1115-30-6
- Molecular formula:
- C10H16O5
- IUPAC Name:
- 1,4-diethyl 2-acetylbutanedioate
- Details on test material:
- - Name of test material (as cited in study report): "Diethylacetylsuccinate"- Physical state: Bright yellow liquid.- Lot/batch No.: SLBE 101- Solubility in water: ca. 15g/L- Melting point: -8 °C- Boiling point: 180 °C/67 mbar- Density: 1.081- Expiration date of the lot/batch: December 1999- Storage condition of test material: In the refrigerator, in the dark.
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS- Source: J. Schitkovits, A-2620 Natschbach- Weight at study initiation: Animal No. 1: 3.4 kg, Animal No. 2: 2.8 kg, Animal No. 3: 2.8 kg- Weith at study termination: Animal No. 1: 3.5 kg, Animal No. 2: 3.0 kg, Animal No. 3: 2.8 kg- Housing: Optimal hygienic conditions, individual caging in metal wire cages, Ehret GmbH, type KK016R, 79 cm x 59 cm x 35 cm- Diet: Altromin 2023 diet for rabbtis, ad libitum.- Water: tap water from an automatical watering system, ad libitum.- Acclimation period: 10 days (animal No. 51) and 16 days (animals No. 52 and No. 53)ENVIRONMENTAL CONDITIONS- Temperature (°C): average of 20 °C - Humidity (%): average of 55 %.- Air changes (per hr): 12 per hour.- Photoperiod (hrs dark / hrs light): 12 hrs dark, 12 hrs light.
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- not required
- Amount / concentration applied:
- 0.1 mLof the test substance was administered to the conjuctival sac of the right eye. The eye was held closed for about one second to prevent loss of the test substance.
- Observation period (in vivo):
- Both eyes of the animals were examined within 24 hours before instillation and apporximately 1, 24, 48 and 72 hours p.a.
- Number of animals or in vitro replicates:
- 3 females
- Details on study design:
- 0.1 mL of the test substance was administered per animal into the conjunctival sacs of the right eyes by gently pulling the lower lids away from the eyeballs for instillation. The eyes were held closed for about one second to prevent loss of test substance.The left eyes remained untreated and served as a control.Firstly, the test substance was administered to one animal. As no evidence for a serious damage to the eye of this animal was found during the initial 72 hour observation period (no corrosive effect), the test substance was administered to the other two animals subsequently.Both eyes of the animals were examined within 24 hours before the instillation and approximately 1, 24, 48 and 72 hours p.a.No further examinations were performed thereafter. The whole eyes, especially the corneae, the irises and the conjunctivae were examined using an otoscope lamp. Eye irritation was scored and recorded using the scheme for eye lesions (see below).
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Remarks:
- all animals
- Time point:
- other: 1, 24, 48, 27 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: not applicable
- Irritation parameter:
- iris score
- Basis:
- mean
- Remarks:
- all animals
- Time point:
- other: 1, 24, 48, 72 h
- Score:
- 0
- Max. score:
- 2
- Reversibility:
- other: not applicable
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #1
- Time point:
- other: 1, 24, 48, 72 h
- Score:
- 0
- Max. score:
- 3
- Reversibility:
- other: not applicable
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #2
- Time point:
- other: 1 h p.a.
- Score:
- 1
- Max. score:
- 3
- Reversibility:
- fully reversible
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #2
- Time point:
- other: 24, 48, 72 h
- Score:
- 0
- Max. score:
- 3
- Reversibility:
- other: not applicable
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #3
- Time point:
- other: 1 h and 24 h p.a.
- Score:
- 1
- Max. score:
- 3
- Reversibility:
- fully reversible
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #3
- Time point:
- other: 48, 72 h
- Score:
- 0
- Max. score:
- 3
- Reversibility:
- other: not applicable
- Irritation parameter:
- chemosis score
- Basis:
- mean
- Remarks:
- all animals
- Time point:
- other: 1, 24, 48, 72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: not applicable
- Irritant / corrosive response data:
- All untreated eyes ("control eyes") were normal at any observation time.Cornea and Iris: only scores of 0 (normal) were noted in all animals at all examination terms.Conjunctivae, redness: 2/3 were affected 1 h p.a. with a score of 1, 1/3 animals had a score of 1 also 24 h p.a.Conjunctivae, chemosis: No lesions were seen at the observation terms.
- Other effects:
- None
Any other information on results incl. tables
| Mean Scores (24, 48, 72 h) for Animal No.: | ||
| 1 | 2 | 3 |
Cornea: | 0.0 | 0.0 | 0.0 |
Iris: | 0.0 | 0.0 | 0.0 |
Conjunctivae, redness: | 0.0 | 0.0 | 0.3 |
Conjunctivae, chemosis: | 0.0 | 0.0 | 0.0 |
Individual data
Examination of corneae, irises and conjunctivae. Individual data and means.
|
|
| Conjunctivae | |||||||||
Time after | Corneae | Irises | Redness | Chemosis | ||||||||
| 1 | 2 | 3 | 1 | 2 | 3 | 1 | 2 | 3 | 1 | 2 | 3 |
1 h | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
24 h | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
48 h | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
72 h | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
mean | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.3 | 0.0 | 0.0 | 0.0 |
Applicant's summary and conclusion
- Interpretation of results:
- not irritating
- Remarks:
- Migrated informationCriteria used for interpretation of results: EU
- Conclusions:
- No classification of "Diethylacetylsuccinate" is derived from the results of this study.
- Executive summary:
The aim of this study was to investigate possible irritating or corrosive effects of the test substance following a single administration into the conjunctival sac of one eye of rabbits.
Methods
Methods and investigations were performed in accordance with the OECD-Guideline 405 and the Directive 92/69/EC, method B.5.
Administration of the test substance
0.1 mL of "diethylacetylsuccinate" was instilled into the conjunctival sac of one eye of each of 3 New Zealand White rabbits. Firstly the test substance was administered to one animal.
Investigations
· Body weights: at the start and at the termination of the test.
· General signs of toxicity: once daily.
· Eye examination: 1, 24, 48 and 72 h after the administration (p.a.) of the test substance.
Results
General signs
No other than ocular lesions were noted.
Eye examination
· Corneae and irises: Not affected at any observation point.
· Conjunctivae: Some injected blood vessels were observed in 2/3 animals 1 h p.a. and lasted until a maximum of 24 h p.a. No lesions were observed at the other examination terms.
Conclusion
The following mean scores were calculated for each animal from the individual examinations performed 24 h, 48 h and 72 h p.a.:
Mean Scores for Animal No.:
1
2
3
Cornea:
0.0
0.0
0.0
Iris:
0.0
0.0
0.0
Conjunctivae, redness:
0.0
0.0
0.3
Conjunctivae, chemosis:
0.0
0.0
0.0
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.