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Diss Factsheets
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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
In accordance with OECD 209 (2010) and according to GLP principles the influence of the substance on the respiration rate of activated sludge was investigated after a contact time of 3 hours. The substance was not toxic to waste water (activated sludge) bacteria at a concentration of 1000 mg/L (NOEC). This corresponds to a NOEC of 390 mg/L expressed as solid content. The 3h-EC50 was determined to be approximately 2600 mg/L, which corresponds to a 3h-EC50 of approximately 1000 mg/L expressed as solid content. In addition, in several ready biodegradability studies it was found that the substance is not toxic to the inoculum (up to 94.2 mg/L).
Key value for chemical safety assessment
- EC50 for microorganisms:
- 2 600 mg/L
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
In accordance with OECD 209 (2010) the influence of the substance on the respiration rate of activated sludge was investigated after a contact time of 3 hours. Two experiments were performed. In experiment 1, a combined limit/range-finding test, concentrations of 10, 100 and 1000 mg/l, corresponding to 3.9, 39 and 390 mg solid/l, were tested. The highest concentration was tested in triplicate, lower concentrations consisted of one replicate. In addition, blank-controls and a nitrification control of the sludge were included. Furthermore, an abiotic control and the highest concentration with a nitrification inhibitor were tested. In experiment 2, a limit test, a concentration of 2600 mg/l, corresponding to 1000 mg solid/l was tested. The concentration was tested in triplicate. In addition, a blank-control (2 replicates) was included. The combined limit/range-finding test showed no inhibition of the respiration rate at all tested concentrations. There was no significant oxygen uptake from abiotic processes and the results at 1000 mg/l with a nitrification inhibitor showed a very slight heterotrophic inhibition of the respiration rate. The limit test showed 48% inhibition of the respiration rate. Thus, based on the first experiment the NOEC was determined to be 1000 mg/l, corresponding to 390 mg solid/l and based on the second experiment the EC50 was estimated to be approximately 2600 mg/l, corresponding to 1000 mg solid/l. The batches of activated sludge were checked for sensitivity in both experiments by testing the reference substance 3,5-dichlorophenol, which showed normal sensitivity.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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