Silicon boride oxide

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

September - October 2011

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP and guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2011

Report date:

2011

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EPA OPPTS 870.1300 (Acute inhalation toxicity)

Test guideline 3

Qualifier:

according to guideline

Guideline:

other: Japan MAFF 8147

GLP compliance:

yes (incl. QA statement)

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Boron silicate

EC Number:

603-157-9

Cas Number:

12676-29-8

Molecular formula:

H7 B7 Si57 O128

IUPAC Name:

Boron silicate

Details on test material:

Name of the test substance used in the study report: Silicon Boride Oxide
Purity: >90%
Expiry date: 29-Jul-2013
Storage Conditions: At room temperature (20 ± 5°C), protected from direct sunlight

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

Age at exposure: 9 weeks
Body weight range at exposure: males: 240.6 to 258.1 g; females: 163.8 to 189.1 g
Housing conditions: Optimal hygienic conditions (OHC) inside a barrier system. Air-conditioned with 10 - 15 air changes per hour, continuously monitored environment with a temperature range of 22 ± 3°C, a relative humidity range of 30 - 70% and a 12 hour fluorescent light / 12 hour dark cycle.
The animals were housed in groups of 5 of the same sex in Makrolon type-IV cages with wire mesh tops and standard softwood bedding including paper enrichment.
Animals had ad libitum access to a pelleted standard Harlan Teklad 2914C rat maintenance diet, except during the period when the animals were restrained in exposure tubes. Community tap water from Füllinsdorf was available ad libitum in water bottles, except during the period when they were restrained in exposure tubes.

Administration / exposure

Route of administration:

inhalation: aerosol

Type of inhalation exposure:

nose only

Analytical verification of test atmosphere concentrations:

yes

Duration of exposure:

4 h

Concentrations:

5.5 mg/l

No. of animals per sex per dose:

5

Control animals:

no

Statistics:

No statistical analysis was performed as only one group was allocated to the study.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 5.5 mg/L air

Remarks on result:

other: gravimetrically determined mean aerosol concentration.

Mortality:

All animals survived the scheduled observation period.

Clinical signs:

other: Ruffled fur was recorded in all animals from the end of exposure until test day 3. Labored breathing was seen in two males starting during the last hour of exposure and lasting up to test day 2. There were no clinical signs from test day 4 of the observat

Body weight:

From test day 1 to test day 2, slight to marked body weight loss was noted in all males and four females. Stagnation of body weight was observed in the remaining female during this period. Thereafter normal body weight development was recorded in these animals.

Gross pathology:

There were no macroscopic findings.