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EC number: 228-819-0 | CAS number: 6359-98-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- From July 30th to August 02nd, 2001
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with generally accepted scientific standards and described in sufficient detail
- Justification for type of information:
- Justification for Read Across is reported in the endpoint summary and it is further detailed in the report attached to the IUCLID section 13.
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- not specified
- Details on test solutions:
- A stock solution was prepared diluting 156.5 mg/l of test item in demineralized water (giving a concentration of 125 mg/l).
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Scenedesmus subspicatus CHODAT.
- Source: Collection of Culture Algae, Insitute of Plant Physiology of Gottingen University
ACCLIMATION
- Culturing media and conditions: the culture was kept in stock to 8 ° C. Three days before the start of the test was incubated at 8000 lux to promote exponential growth. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Test temperature:
- 21 ± 2 °C
- Nominal and measured concentrations:
- 125 mg/l, nominal
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 8 ml tubes open, agitated individually.
- No. of vessels per concentration: 3 replicates.
- No. of vessels per control: 3 replicates.
EFFECT PARAMETERS MEASURED
Determination of light absorption in the cuvettes at the beginning and end of the test period with spectrophotometer PU 8625 at λ = 440 nm against test batch without algae. In the ranges where the measured, the cell number and the optical density are proportional to each other. - Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 125 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 125 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- The No Observed Effect Level at 72 hours resulted to be lower than 125 mg/l.
The factor 16 required in the control approach within 72 hours corresponds to a growth rate of 0.9. A growth rate of 1.40 was achieved.
The pH of the control batch should not change by more than 1.5 units; in the test, the change was 0.8 units. - Conclusions:
- ErC50 (72h) > 125 mg/l (nominal)
EbC50 (72h): 125 mg/l (nominal) - Executive summary:
Method
The influence of the test item on the growth of the green algal species Desmodesmus subspicatuswas investigated in a 72-hour test according to the OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test).
Result
ErC50 (72h) > 125 mg/l (nominal)
EbC50 (72h): 125 mg/l (nominal)
Reference
Extinctions
Nominal conc. (mg/l) | E (0h) | E (24h) | E (48h) | E (72h) |
0 | 6 | 54 | 193 | 386 |
0 | 6 | 55 | 205 | 392 |
0 | 6 | 50 | 161 | 351 |
120 | 6 | 20 | 92 | 230 |
120 | 6 | 12 | 97 | 233 |
120 | 6 | 17 | 94 | 229 |
Data obtained
Nominal conc. (mg/l) | Biomass | Growth rate | Average of inhibition (%) | |||
Value | Inhibition |
Value | Inhibition |
Biomass | Growth rate | |
0 | 425.00 | -3.03 | 1.41 | -0.63 | 0! | 0! |
0 | 441.00 | -6.91 | 1.41 | -1.00 | ||
0 | 371.50 | 9.94 | 1.38 | 1.64 | ||
120 | 212.00 | 48.61 | 1.23 | 11.73 | 48.8 | 11.7 |
120 | 210.50 | 48.97 | 1.24 | 11.42 | ||
120 | 210.50 | 48.97 | 1.23 | 11.83 |
Description of key information
ErC50 (72h) > 125 mg/l (nominal)
EbC50 (72h): 125 mg/l (nominal)
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 125 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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