Reaction mass of 1,2-Diacetoxy-3-butene and 1,2-Dihydroxy-3-butene, monoacetate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP guideline study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

445-630-0

EC Name:

-

Molecular formula:

Unspecified

IUPAC Name:

1-(acetyloxy)but-3-en-2-yl acetate; 2-hydroxybut-3-en-1-yl acetate

Details on test material:

- Test substance: Vinylglykoldicatet ca. 75% ig
- Batch No.: Kanister 1,2,4 und 5

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

- Test organism: Danio rerio (formerly Brachydanio rerio)
- Common name: zebra fish
- Supplier: Zierfisch-Center Klöckner, Ludwigshafen
- Body length: 2.6 cm (range 2.3 - 2.9 cm)
- Body weight: 0.14 g (range 0.11 - 0.2 g)

Housing and adaption
- Culture conditions: oil-free aerated and charcoal filtered tap water, flow-through system
- Water temperature: Approx. 23 - 25 °C
- Duration of housing: 14 days
- Mortality during the last week prior test start: 0 %
- Medical treatment: none
- Diet: Tetramin fish food daily, ad libitum, additionally live brine shrimp (artemia) on workdays

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

ca. 2.5 mmol/l

pH:

7.7 - 8.0

Dissolved oxygen:

5.3 - 8.9

Details on test conditions:

Test procedure
- Test water: Dechlorinated and charcoal-filtered tap water, aerated.
- Total hardness: approx. 2.5 mmol/l
- Acid capacity: approx. 5.5 mmol/l
- Ratio Ca/Mg ions:approx. 8:1
- pH: generally 7.5 - 8.5
- Volume of water:10 l
- No. of animals per test concentration and control: 10
- Loading rate (g fish/l test water): 1.8
- Test vessels: 10 l glass flasks
- Test temperature: 24 °C
- Adaption to test water and test temperature: 14 days
- Photoperiod: 16 : 8 hours day-night regime
- Light intensity: approx. 82 - 280 lux
- Aeration: None
- Exposure period: 96 h
- Test media renewal period: 48 h
- pH during the test: 7.7 - 8.0
- Oxygen content during the test: 5.3 - 8.9 mg/l
- Withdrawal of food: 1 day before and during exposure.
- Nominal test concentrations: 1, 2.2, 5, 10, 22 and 50 mg/l (based on the results of a range-finding test). In addition, a control was tested in parallel.
- Observation time: 1, 4, 24, 48, 72 and 96 h.
- Endpoints investigated: mortality and symptoms of intoxication.
- Preparation of the test item: The test concentrations were freshly prepared separately for each test vessel. The test substance was weighed in to a beaker. The defined volume of test water was filled in to the test vessel. An aliquot from the test water in the test vessel was taken and mixed with the test substance using a glass rod and the mixture was poured into the test vessel and the whole test batch was homogenized again with a glass rod.
- Analytical dose verification: Samples from each test concentration and the control at the start and the end of the two intervals were taken and analysed using HPLC-method.
- Statistical evaluation of data: The LC50 was determined using probit analysis. The NOEC, the LC0, the LOEC and the LC100 were determined directly from the raw data.

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

10.9 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

30.9 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

6 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

6 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

RS-Freetext:
During the 96 h exposure to vinylglykoldicatetat 75% ig, mortalities were as follows:

Nominal test Cumulative mortalitiy (%) after x hours
concentration 1 / 4 / 24 / 48 / 72 / 96
(mg/l)
Control 0 / 0 / 0 / 0 / 0 / 0
1 0 / 0 / 0 / 0 / 0 / 0
2.2 0 / 0 / 0 / 0 / 0 / 0
5 0 / 0 / 0 / 0 / 0 / 0
10 0 / 0 / 0 / 0 / 0 / 0
22 0 / 0 / 0 / 3 / 5 / 7
50 0 / 0 / 10 / 10 / 10 / 10

Symptoms of intoxication:

Nominal test Symptoms of intoxication after x hours
concentration 1 / 4 /24 / 48 / 72 / 96
(mg/l)
Control - / - / - / - / - / -
1 - / - / - / - / - / -
2.2 - / - / - / - / - / -
5 - / - / - / - / - / -
10 - / - / - / - / - / -
22 - / - / - / - / - / -
50 - / - / + / + / + / +

Definitions of Symptoms:
-: No symptoms
+: All fish dead

Analytical results:

Nominal test concentration / mean measured concentration / recovery (% of nominal)
(mg/l)
Control n.d. -
1 0.6 60.0
2.2 1.3 59.1
5 2.9 58.0
10 6.0 60.0
22 12.5 56.8
50 30.9 61.8

n.d. - not detectable

The analytical results were out of +/- 80 % of the nominal values. Therefore, all biological results were refered to mean measured test concentrations of vinylglykoldiacetat 75% ig.

Behavior of the test item during the test:
No remarkable observations.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes