Aluminium, 6-hydroxy-5-[(2-methoxy-5-methyl-4-sulfophenyl)azo]-2-naphthalenesulfonic acid complex

Endpoint summary

Description of key information

Short term toxicity to fish:

Based on mortality of fish due to the exposure of test chemical  at nominal concentration 100 mg/l, experimental median lethal Concentrations [LC-50 (96 h)] on Zebra fish (Danio rerio) was determine to be > 100 mg/l.

Short term toxicity to aquatic invertebrates:

Determination of the inhibition of the mobility of daphnids was carried out with the test chemical according to OECD Guideline 202.

A limit test at sample concentration of 100 mg/L was performed. Effects on immobilisation were observed for 48 hours. At 100 mg/l only 8% inhibition was observed, thus it can be concluded that the EC50 was >100 mg/l.

The median effective concentration (EC50) for the test chemical, in Daphnia magna was determined to be >100 mg/L for immobilisation effects. Based on this EC50 value and after comparing with CLP criteria for aquatic classification of the substance it is concluded that the chemical does not exhibit short term toxicity to aquatic invertebrate (Daphnia Magna) and cannot be classified as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria:

After 72 hours of exposure to test chemical to various nominal test concentration, EC50 calculated from equation through probit analysis was determine to be 136.57 mg/L.

Toxicity to microorganisms:

The test substance shows moderate to negligible toxicity of inhibition range 1375 mg/l - 10000 mg/l.

Additional information

Short term toxicity to fish:

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test chemical. The stock solution was prepared as 400mg/4L, with the concentration of 100mg/L, and was kept for 24 hr stirring. After this filter the stock, give it for analytical detection and then stock taken for experiment.

The test was performed on the limit concentration i.e nominal concentration selected for the experiment were 100 mg/L and test fish were exposed to these concentration for 96 hours. After 96hrs of exposure LC0, LC50 and LC100 was observed. Effect on the symptoms and the normal activity was checked in the interval of 24rs. pH, tempterature and dissolved oxygen was also checked.

The lethal concentrations LC50 was determine to be >100 mg/L

LC0 (96 hours) (highest loading at which no mortality was observed) = 100 mg/L

LC50 (96 hours) Experimental = >100 mg/L

LC100 (96 hours) (lowest loading at which 100% mortality was observed) =No mortality was observed

Thus based on the LC50 it can be concluded that the test chemical was nontoxic and can be consider to be not classified as per the CLP classification criteria.

Short term toxicity to aquatic invertebrates:

Determination of the inhibition of the mobility of daphnids was carried out with the test chemical according to OECD Guideline 202.

A limit test at sample concentration of 100 mg/L was performed. Effects on immobilisation were observed for 48 hours. At 100 mg/l only 8% inhibition was observed, thus it can be concluded that the EC50 was >100 mg/l.

The median effective concentration (EC50) for the test chemical, in Daphnia magna was determined to be >100 mg/L for immobilisation effects. Based on this EC50 value and after comparing with CLP criteria for aquatic classification of the substance it is concluded that the chemical does not exhibit short term toxicity to aquatic invertebrate (Daphnia Magna) and cannot be classified as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria:

The effect of test chemical was studied on the growth of fresh water green alga Chlorella vulgaris. The study was conducted following OECD guideline 201- Alga, growth inhibition test. The test concentration chosen for the study were 6.25mg/L, 12.5mg/L, 25mg/L, 50mg/L, 100mg/L, 200mg/L. The test solution was prepared in aseptic condition. The test chemical was prepared by adding 50 mg of test item in 250 ml of BBM to get the final concentration of 200 mg/L. This stock solution was kept for stirring for 24 hours and filter it to obtain a homogenous solution for the experiment. The test concentrations were chosen according to the available data of the test item. The concentrations chosen were set up to the water solubility limit. The remaining test solutions were prepared by dilution from the above stock solution. To have a better growth and visibility of cells, the initial of the culture was kept 1 X 104cells/ml.

The microscopic observations were noted down in each of the control vessel. All the cells appeared healthy, round and green throughout the study duration in the control. Also, the drift in pH in the control vessels did not increase by >1.5 units when observed on 72 hours as compared to 0 hours. The average pH drift observed in the control vessels was 0.1 units.The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of the test item. EC50 calculated graphically through probit analysis was observed to be 136.57 mg/L.

Based on the EC50, it can be concluded that the chemical was nontoxic and can be consider to be not classified as toxic as per the CLP classification criteria.

Toxicity to microorganisms:

Various studies available for the test chemical were reviewed to determine the toxic nature of test chemical on the growth and other activity of microorganisms. The studies are as mentioned below:

 

In the first study the death of Paramecium caudatum (PC), a unicellular animal, can be observed more readily and in far less time than that of small animals. Hence a bioassay was conducted to study the toxic effect of Test chemical. Paramecium Caudatum was maintained at 22°C on 0.15 % dried lettuce infusion and fed with Aerobacter aerogenes. Chemical was tested in 0.1% and 1% concentration. The test concentrations were put in a hollow slide glass, and an equal volume of 0.04 M phosphate buffer, pH 7.0, was added. After 5 to 10 test organisms were added, their survival times were measured microscopically. Thirty to forty test organisms for each concentration were tested by the same method, and the mean survival time and the death rate were calculated. The survival time was defined as the time required until death was observed for each concentration. Death was assumed to have occurred when there was no movement. The death rate was defined as the percentage of deaths observed during 20 minutes. The mean survival time (in sec) of test organism Paramecium caudatumwas determined to be 695 seconds.  The death rate of the test organism at 10000 mg/l was 77.4%. Therefore the Effective concentration causing more than 50% death of Paramecium caudatum was reported as 10000 mg/l.

 

First study was supported by the second study from peer reviewed journal. The exposure times were 30 s and 30 min, and the peak luminescence value was obtained during the first 5 s after adding the bacterial suspension to the sample. The tested concentrations amounted to 500–5000 mg/l with RO16. The results were calculated as the inhibition % of light production and expressed using the corresponding EC50 values. With 30-min exposure time, EC50 values is determine to be 1375 mg/L for the test chemical. Thus based on the EC50 value, chemical consider to be nontoxic to the growth of microorganism.

 

Thus based on the overall studies for the test chemical, chemical consider to be nontoxic and the toxicity ranges from the concentration 1375 mg/l - 10000 mg/l.