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EC number: 807-621-3 | CAS number: 1428450-95-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 18 Feb - 21 Feb 2014
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 014
- Report date:
- 2014
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Landesamt für Umwelt, Wasserwirtschaft und Gewerbeaufsicht, Mainz, Germany
Test material
- Reference substance name:
- N-[4-(2,4-dihydroxyphenyl)-1,3-thiazol-2-yl]-2-methylpropanamide
- EC Number:
- 807-621-3
- Cas Number:
- 1428450-95-6
- Molecular formula:
- C13H14N2O3S
- IUPAC Name:
- N-[4-(2,4-dihydroxyphenyl)-1,3-thiazol-2-yl]-2-methylpropanamide
- Test material form:
- solid: particulate/powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Control, 0, 0.46, 1.0, 2.2, 4.6 and 10 mg/L
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A stock solution containing 10.5 mg/L in nutrient medium (demineralised water enriched with minerals but without algae) was prepared. For this the nominal load of test item was solved with the help of an ultrasonic bath. Because after 30 min the resulting solution was not complete clear, the solution was filtrated through 0.45 µm Teflon filters. The lower treatments were prepared by dilution of the stock solution with nutrient medium.
- Eluate: no
- Differential loading: no
- Controls: yes, test medium control
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: green algae
- Strain: 86.81 (SAG Strain Number)
- Source: The culture of Desmodesmus subspicatus was obtained in January 2013 from MBM Sciencebridge GmbH (Institut für Pflanzenphysiologie of Universität Göttingen, Germany). The algae are kept as stock culture on solid agar at 7 °C.
ACCLIMATION
- Acclimation period: Four days before the start of the test, an aliquot of the permanent culture was brought into pre-culture medium and incubated for 96 h. The resulting culture is growing exponentially.
- Culturing media and conditions: same as test
- Any deformed or abnormal cells observed: no
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 23 °C
- pH:
- 7.7 - 8.2
- Nominal and measured concentrations:
- nominal: control, 0.46, 1.0, 2.2, 4.6 and 10 mg/L
measured: 0, 0.39, 0.72, 1.49, 1.87, 6.91 mg/L (based on the geometric mean measured concentration) - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass flasks
- Material, size, headspace, fill volume: glass, 65 mL, headspace: 20 ± 1 mL, fill volume: 45 ± 1 mL
- Aeration: shaken on an orbital shaker
- Initial cells density: 3822 cells/mL
- Control end cells density: 227197 cells/mL (mean)
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: demineralised water
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Photoperiod: constant illumination
- Light intensity and quality: 5800 lux
EFFECT PARAMETERS MEASURED:
- Determination of cell concentrations: Before the start and every 24 h, the cell number was calculated based on the determination of the light absorption at 440 nm. At the end of the test, the treatments were examined microscopically in order to assess the appearance of the alga and detect abnormalities.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2 - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.39 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.39 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.39 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.33 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.68 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.51 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 3.3 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.73 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): small cells were observed at a nominal concentration of 2.2 mg/L
- Any stimulation of growth found in any treatment: yes, No inhibition but instead stimulation was observed at the nominal concentration of 0.46 mg/L. - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- EC50: EC50 (72 h) 0.89 mg/L based on growth rate; in the range of interlaboratory test (0.60 - 1.03 mg/L) - Reported statistics and error estimates:
- NOEC/LOEC determination: t-test
ECx determination: Software OriginTM. The values for p resp. χ² were calculated. The data were evaluated using sigmoidal fit using a logistic equation.
Any other information on results incl. tables
Table 1: Cell numbers
Nominal concentration [mg/L] |
Measured concentration [mg/L] (geometric mean) |
Replicate |
Cell number/mL |
|||
|
|
|
0 h |
24 h |
48 h |
72 h |
Control |
Control |
1 |
3822 |
14014 |
49686 |
198744 |
2 |
3822 |
14014 |
53508 |
212758 |
||
3 |
3822 |
12740 |
54782 |
256074 |
||
4 |
3822 |
15288 |
54782 |
235690 |
||
5 |
3822 |
14014 |
50960 |
219128 |
||
6 |
3822 |
12740 |
54782 |
240786 |
||
Mean |
3822 |
13802 |
53083 |
227197 |
||
SD |
0 |
959 |
2231 |
20851 |
||
0.46 |
0.39 |
1 |
3822 |
14014 |
64974 |
321048 |
2 |
3822 |
11466 |
64974 |
308308 |
||
3 |
3822 |
14014 |
53508 |
284102 |
||
Mean |
3822 |
13165 |
61152 |
304486 |
||
SD |
0 |
1471 |
6620 |
18767 |
||
1.0 |
0.72 |
1 |
3822 |
14014 |
34398 |
133770 |
2 |
3822 |
14014 |
38220 |
146510 |
||
3 |
3822 |
15288 |
31850 |
122304 |
||
Mean |
3822 |
14439 |
34823 |
134195 |
||
SD |
0 |
736 |
|
12109 |
||
2.2 |
1.49 |
1 |
3822 |
8918 |
16562 |
30576 |
2 |
3822 |
10192 |
17836 |
35672 |
||
3 |
3822 |
10192 |
19110 |
44590 |
||
Mean |
3822 |
9767 |
17836 |
36946 |
||
SD |
0 |
736 |
1274 |
7093 |
||
4.6 |
1.87 |
1 |
3822 |
11466 |
14014 |
33124 |
2 |
3822 |
7644 |
15288 |
29302 |
||
3 |
3822 |
8918 |
16562 |
29302 |
||
Mean |
3822 |
9343 |
15288 |
30576 |
||
SD |
0 |
1946 |
1274 |
2207 |
||
10 |
6.91 |
1 |
3822 |
6370 |
14014 |
24206 |
2 |
3822 |
6370 |
12740 |
19110 |
||
3 |
3822 |
6370 |
16562 |
28028 |
||
Mean |
3822 |
6370 |
14439 |
23781 |
||
SD |
0 |
0 |
1946 |
4474 |
Table 2: Inhibition of growth
Nominal concentration [mg/L] |
Measured concentration [mg/L] (geometric mean) |
Replicate |
Inhibition Growth Rate µ |
|
|
|
72 h |
Control |
Control |
1 |
3.19 |
2 |
1.522 |
||
3 |
-3.02 |
||
4 |
-0.99 |
||
5 |
0.80 |
||
6 |
-1.51 |
||
Mean |
0! |
||
SD |
2.26 |
||
0.46 |
0.39 |
1 |
-8.56 |
2 |
-7.57 |
||
3 |
-5.56 |
||
Mean |
-7.23 |
||
SD |
1.53 |
||
1.0 |
0.72 |
1 |
12.89 |
2 |
10.66 |
||
3 |
15.09 |
||
Mean |
12.88 |
||
SD |
2.21 |
||
2.2 |
1.49 |
1 |
49.05 |
2 |
45.25 |
||
3 |
39.81 |
||
Mean |
44.71 |
||
SD |
4.65 |
||
4.6 |
1.87 |
1 |
47.09 |
2 |
50.09 |
||
3 |
50.09 |
||
Mean |
49.09 |
||
SD |
1.73 |
||
10 |
6.91 |
1 |
54.78 |
2 |
60.57 |
||
3 |
51.18 |
||
Mean |
55.51 |
||
SD |
4.73 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- A NOEC of 0.39 mg/L and a LOEC of 0.72 mg/L was statistically determined for the endpoints.
The EC10s were determined as 0.33 mg/L (Growth Rate), 0.51 mg/L (AUC) and
0.68 mg/L (Yield). The EC50s were determined as 3.3 mg/L (Growth Rate), 1.0 mg/L
(AUC) and 0.73 mg/L (Yield). - Executive summary:
One experiment was performed.
The study was performed using five concentrations ranging from 0.46 to 10 mg/L nominal
concentration. Incubation time (test system Desmodesmus subspicatus) was 72 hours.
The cell concentration of each replicate was determined by measuring the absorption of
the solutions at 440 nm every 24 hours with a spectral photometer. The cell density of the
cultures was calculated based on the correlation curve between the adsorption and the cell
density of the cultures determined by microscope counts. Growth rate μ, area under the
growth curve (AUC1) and the yield2 were determined from the cell densities at the respective
observation times.
Significant inhibition of algal growth was observed at the following concentrations:
1 / 2.2 / 4.6 / 10 mg/L
At the start and at the end of the test, the content of the test item in the test solutions was
determined using HPLC-UV. The measured concentrations lay in the range of 37 % and
99 % of the nominal concentrations. Therefore, the determination of the results was based
on the geometric mean of the measured concentrations.
The EC50s of potassium dichromate were tested in a separate reference test. The values
lay within the normal range of the laboratory.
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