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EC number: 210-112-3 | CAS number: 606-28-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- February 2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Identification: RCX 14-672
Chemical name: methyl-2-benzoylbenzoate
Batch no.: N14003
CAS no.: 606-28-0
EC no.: 210-112-3
Molecular formula: C15H12O3
Molecular mass: 240.3 g/mol
Description: white to light yellowish powder
Purity: >99% (gas chromatography)
Water solubility: 117.7 mg/l
Test item storage: at room temperature, protected from light
Stability: stable under storage conditions
Expiry date: 30 November 2015 - Analytical monitoring:
- not required
- Vehicle:
- no
- Details on test solutions:
- The test item was directly weighed into the test vessels containing natural water, in an amount corresponding to achieve the final test concentrations.
A 100-fold concentrated synthetic sewage (32 ml/l of in the final mixture) was then added, followed by the activated sludge suspension (1.7 g/l dry matter in the final mixture). - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Not adapted and not pre-conditioned activated sludge from the aeration tank of the ARA Werdhölzli (CH-8048 Zürich), a municipal biological waste water treatment plant. 1.7 g/l dry matter in the final mixture.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- No post-exposure observation period
- Hardness:
- Total hardness: 206-242 mg/l
- Test temperature:
- 22 ± 2 °C
- pH:
- 6.9-7.4
- Dissolved oxygen:
- The oxygen concentration, in mg/l, was measured with an oxygen electrode (ORION 97-08 equipped with an ORION SA 720 transformer; Hügli-Labortec AG, CH-9030 Abtwil). The electrode is inserted into a 300 ml BOD bottle and the oxygen concentration is recorded for up to 10 min.
- Salinity:
- 16.1 mg/l
- Nominal and measured concentrations:
- Based on the results of the non-GLP preliminary test , with a loading rate of 100 mg/l of RCX 14-672, the definitive test was performed as a screening test with the following loading rates: 100, 316 and 1000 mg/l.
- Details on test conditions:
- - Test units: 1000 ml flasks containing a total volume of test suspension of 500 ml; aerated by shaking (150 r.p.m.)
- Measuring apparatus: 300 ml flat bottom BOD flasks; polarographic oxygen electrode, connected to a potentio-metric recorder
- Duration/contact time: 3 hours in a temperature-controlled room
- Temperature: 22 ± 2 °C
- Test medium: synthetic sewage feed prepared with tap water:16 ml of 100-fold sewage concentrate per 500 ml of final test mixture - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- The following respiration inhibition of the activated sludge compared to the blank controls was observed: 10% at 1000 mg/l, 8% at 316 mg/l and 7% at 100 mg/l.
Based on these data, the EC10 value of RCX 14-672 was estimated to be 1000 mg/l loading rate. The EC20, EC50 and EC80 values were all estimated to be >1000 mg/l loading rate. The no observed effect concentration (NOEC) was <100 mg/l as determined by Dunnett’s test. - Results with reference substance (positive control):
- The EC50 value of the reference item 3,5-dichlorophenol was estimated to be 5.5 mg/l.
- Validity criteria fulfilled:
- yes
- Executive summary:
The inhibitory effect of RCX 14-672 on the oxygen consumption of activated sludge microorganisms of a municipal sewage treatment plant was investigated under aerobic static conditions over an exposure time of 3 hours. The inhibitory effect may include the effect on respiration and nitrification. The loading rates of RCX 14-672 were 100, 316 and 1000 mg/l.
No chemical analysis of test concentrations was conducted. For the estimation of the effective concentration leading to 10, 20, 50, and 80% respiration inhibition (EC10, EC20, EC50, EC80) the loading rates of the test item were used, assuming the test item to be stable in water over 3 h.
The following respiration inhibition of the activated sludge compared to the blank controls was observed: 10% at 1000 mg/l, 8% at 316 mg/l and 7% at 100 mg/l. Based on these data, the EC10 value of RCX 14-672 was estimated to be 1000 mg/l loading rate. The EC20, EC50 and EC80 values were all estimated to be >1000 mg/l loading rate. The no observed effect concentration (NOEC) was <100 mg/l as determined by Dunnett’s test
Reference
Description of key information
The 3-hour EC50 for microorganisms is higher than 1000 mg/L, which is the highest dose level used in the study.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
- EC10 or NOEC for microorganisms:
- 100 mg/L
Additional information
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