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Diss Factsheets
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EC number: 942-925-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- Principles of method if other than guideline:
- Male and female rats were exposed nose-only to 2590 mg (maximum attainable concentration) of the test substance/m³ for 4 hours. Mortality, clinical signs, reflex measurements rectal temperature and body weights were examined during the 14 days post observation period. A gross-pathological examination was performed on day 14 after exposure to the test item. To identify exposure-related effects, comparisons with vehicle control were performed. This control was exposed to an atmosphere using essentially similar exposure conditions as were used for the test item.
- GLP compliance:
- yes
- Test type:
- standard acute method
Test material
- Reference substance name:
- zinc, 5,5'-azobis-2,4,6(1H,3H,5H)-pyrimidinetrione complexes and melamine
- IUPAC Name:
- zinc, 5,5'-azobis-2,4,6(1H,3H,5H)-pyrimidinetrione complexes and melamine
- Test material form:
- aerosol dispenser: not specified
- Remarks:
- migrated information: aerosol
- Details on test material:
- Test item: Zinc-Azo barbituric acid / Melamine used as micronized
Chemical name: Reaction mass of zinc, 5,5'-azobis-2,4,6(1 H,3H,5H)-pyrimidinetrione complexes and melamine
CAS name: Zinc, 5,5'-azobis-2,4,6(1 H,3H,5H)pyrimidinetrione complexes and melamine
CAS number: 264233-58-1
Empirical formula: C8H4N6O6Zn x 2 H20 and C3H6N6
Stability: Stability certified for the duration of study
Storage conditions: room temperature
Appearance: solid, red-brown powder
Purity: 96.5 %
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
Administration / exposure
- Route of administration:
- inhalation: aerosol
- Type of inhalation exposure:
- nose only
- Vehicle:
- air
- Analytical verification of test atmosphere concentrations:
- yes
- Duration of exposure:
- 4 h
- Concentrations:
- 0 mg/m³ (control group)
2590 mg/m³ (test group); 2590 mg/m³ was the maximum attainable concentration) - No. of animals per sex per dose:
- N /Group/sex Concentration (mg/m³)
1/m 0
2/m 2590
1/w 0
2/w 2590 - Control animals:
- yes
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- >= 2 590 mg/m³ air
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Remarks on result:
- other: 2590 mg/m³ was the maximum attainable concentration.
Any other information on results incl. tables
One group of female and male rats was nose-only exposed to the solid aerosol of the test item at the maximum attainable concentration of 2590 mg/m³ in the animals breathing zone (limit test). In order to achieve the required mass median aerodynamic diameter (MMAD) the test item was micronized using a ball-mill. Rats of the control group were exposed to air under otherwise identical circumstances.
The results can be summarized as follows:
The respirability of the aerosol was adequate and in compliance of test guidelines [the mass median aerodynamic diameter (MMAD) was 3.86 µm, the geometric standard deviation (GSD) was 2.59]. Efforts have been made to generate the maximum attainable concentration of 2590 mg/m³ test item in consideration of particle size distribution according OECD test guideline 403.
Mortality did not occur during the course of the study. All rats exposed to 2590 mg/m³ revealed clinical symptoms (e.g. bradypnea, labored breathing, irregular breathing, motility reduced, atony, high-legged gait, cyanosis, piloerection, haircoat ungroomed, fur orange stained, reduced reflexes). Furthermore animals exposed to the test item showed significant reduction in incremental body weight gain and absolute body weight when compared to control animals as well as significant decreased body temperatures in comparison to controls. Gross pathological findings were seen in all six animals exposed to 2590 mg/m3 on day 14 (light-colored areas and orangecolored areas in the lungs, orange discolorations in the lung-associated lymph nodes).
In summary, there is evidence of low acute inhalation toxicity in rats after exposure (4h) of the aerosolized test item. The LC50 is> 2590 mg/m³.
Applicant's summary and conclusion
- Executive summary:
A study on the acute inhalation toxicity of Zinc-Azo barbituric acid / Melamine (henceforward referred to as test item) in rats has been conducted in accordance with OECD TG#403 (2009). Test procedures were adapted so as to comply also with the EU Directive 92/69/EEC, and especially OECD GD#39 (2009). One group of female and male rats was nose-only exposed to the solid aerosol of the test item at the maximum attainable concentration of 2590 mg/m³ in the animals breathing zone (limit test). In order to achieve the required mass median aerodynamic diameter (MMAD) the test item was micronized using a ball-mill. Rats of the control group were exposed to air under otherwise identical circumstances.
The results can be summarized as follows:
The respirability of the aerosol was adequate and in compliance of test guidelines [the mass median aerodynamic diameter (MMAD) was 3.86 µm, the geometric standard deviation (GSD) was 2.59]. Efforts have been made to generate the maximum attainable concentration of 2590 mg/m³ test item in consideration of particle size distribution according OECD test guideline 403.
Mortality did not occur during the course of the study. All rats exposed to 2590 mg/m³ revealed clinical symptoms (e.g. bradypnea, labored breathing, irregular breathing, motility reduced, atony, high-legged gait, cyanosis, piloerection, haircoat ungroomed, fur orange stained, reduced reflexes). Furthermore animals exposed to the test item showed significant reduction in incremental body weight gain and absolute body weight when compared to control animals as well as significant decreased body temperatures in comparison to controls. Gross pathological findings were seen in all six animals exposed to 2590 mg/m3 on day 14 (light-colored areas and orangecolored areas in the lungs, orange discolorations in the lung-associated lymph nodes).
In summary, there is evidence of low acute inhalation toxicity in rats after exposure (4h) of the aerosolized test item. The LC50 is> 2590 mg/m³.
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