[29H,31H-phthalocyaninetrisulphonyl trichloridato(2-)-N29,N30,N31,N32]copper

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The test was conducted according to relevant guidelines.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

other: Directive 88/302/EEC

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

[29H,31H-phthalocyaninetrisulphonyl trichloridato(2-)-N29,N30,N31,N32]copper

EC Number:

248-236-5

EC Name:

[29H,31H-phthalocyaninetrisulphonyl trichloridato(2-)-N29,N30,N31,N32]copper

Cas Number:

27121-30-8

Molecular formula:

C32H13Cl3CuN8O6S3

IUPAC Name:

Copper, [29H,31H-phthalocyanine-C,C,C-trisulfonyl trichloridato(2-)-kN29,kN30,kN31,kN32]-

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

A stock solution (nominal concentration 2000 mg/L) was prepared. 200 mg/L test were placed in a beaker in mixed with 800 mL water. After stirring for 1.5 hours at 19°C, the test solution was adjusted with diluted sodium hydroxide from pH 2.1 to 7.0 und filled up to 1 L.
Nominal concentrations of 250 mg/L, 500 mg/L and 1000 mg/L were tested.

Test organisms

Test organisms (species):

activated sludge, domestic

Details on inoculum:

Source: municipal sewage treatment works of Frankfurt/M-Sindlingen
Pretreatment: according to the guideline
Acclimatization: Not pre-adapted in the laboratory to the sumission substance or the reference substance

Study design

Limit test:

no

Test conditions

Test temperature:

20°C ± 2°C

pH:

7.2 ± 0.2

Nominal and measured concentrations:

nominal concentrations: 250 mg/L, 500 mg/L, 1000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass beakers, diameter 60 mm, height 170 mm, volume appr. 480 mL

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol: 5, 20, 30 mg/L

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

3 h

Dose descriptor:

other: EC20

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 3

Duration:

3 h

Dose descriptor:

other: EC80

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Details on results:

The submission substance is not harmful to activated sludge micororganisms. The EC50 is in the range of >1000 mg/L (nominal concentration).

Results with reference substance (positive control):

The EC50 (3 hours) for the reference substance was in the required range of the guideline.

Reported statistics and error estimates:

no data

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In an activated sludge respiration inhibition test, the submission substance proved to be innooxious to activated sludge microorgansims. The EC50 (3 hours) for respiration inhibition had been determined to be >1000 mg/L.

Executive summary:

In an activated sludge respiration inhibition test, the submission substance proved to be nontoxic to activated sludge microorgansims. The test was conducted according to relevant guidelines. However, documentation is limited (reliability 2). The EC50 (3 hours) for respiration inhibition had been determined to be >1000 mg/L.