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EC number: 209-693-6 | CAS number: 590-90-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2015-03-23 - 2015-03-25
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study according to OECD guideline
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 4-hydroxybutan-2-one
- EC Number:
- 209-693-6
- EC Name:
- 4-hydroxybutan-2-one
- Cas Number:
- 590-90-9
- Molecular formula:
- C4H8O2
- IUPAC Name:
- 4-hydroxybutan-2-one
- Reference substance name:
- 2-Butanone, 4-hydroxy-
- IUPAC Name:
- 2-Butanone, 4-hydroxy-
- Details on test material:
- - Name of test material (as cited in study report): 4-Hydroxybutan-2-one
- Physical state: colourless or light yellow liquid
- Lot/batch No.:10781727
- Expiration date of the lot/batch: November 04, 2016
- Other: miscible with water at room temperature
Constituent 1
Constituent 2
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: one limit concentration 100 mg/L plus the control
- Sample storage conditions before analysis: The sample of the test item was stored at ambient temperature without exposure to light in a tightly
sealed container.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
The test item in the amount of 100.70 mg was weighed in a glass crystallizer transferred by multiple washing with Elendt M7 medium into a glass flask, the total volume was filled up to 1007 mL. The test item concentration of 100 mg/L was visually homogeneous
and transparent. After 24 h of exposure the test item concentration and the control were renewed to
freshly prepared.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna Straus
- Source: Institute of Industrial Organic Chemistry, Branch Pszczyna, Department of Ecotoxicology
- Age at study initiation (mean and range, SD): 24 hours old (not first brood progeny)
- Method of breeding: Daphnia magna was cultured in glass beakers with a capacity of 150 mL (one parent per vessel)
containing 100 mL of Elend M7 medium, at room temperature ranging from 18 to 22°C with daily cycle
16 h light : 8 h dark
- Feeding during test: no feeding
ACCLIMATION
- Type and amount of food: suspension of algae; mixture of two species Pseudokirchneriella subcapitata :
Desmodesmus subspicatus (in 2:1 ratio) Group B vitamins and micronutrients necessary for proper growth
were supplied with the lyophilized suspension of Spirulina sp.
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 209 mg Ca CO3/L
- Test temperature:
- 20.4 - 21.9 ºC
- pH:
- 7.31 - 7.46
- Dissolved oxygen:
- 8.65 - 9.01 mg/L
- Nominal and measured concentrations:
- nominal test item concentrations: 100 mg/L plus the control
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers of 150 mL with transparent lids
- Type (delete if not applicable): closed
- Aeration: no aeration.
- Renewal rate of test solution (frequency/flow rate): one renewal
- No. of organisms per vessel: five Daphnia magna in each replicate
- No. of vessels per concentration (replicates): 8
- No. of vessels per control (replicates): 8
TEST MEDIUM / WATER PARAMETERS
- The Elendt M7 medium recommended by the OECD Guideline No. 202 (2004) was used to culture the
test organism and as a diluent/solvent of the test item
OTHER TEST CONDITIONS
- Light intensity:daily cycle 16 h light : 8 h dark; fluorescent light source
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Daphnia magna was observed for immobilization after 24 and 48 h of exposure. The Daphnia magna
was considered immobile if they showed no ability to swim within 15 seconds after swirling the test
vessel
TEST CONCENTRATIONS
- Test concentrations: were determined on the basis of the preliminary tests results - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- EC50 (24h): 0.64 mg/L
EC50 (48h): 0.57 mg/L
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In the definitive test the validity criteria were met according to OECD Guideline No. 202 (2004):
- The percentage of immobilization of Daphnia magna in the control was 5% (criterion: not more
than 10%),
- The dissolved oxygen concentrations in the test vessels were within the range of
8.62 – 9.01 mg/L (criterion: not less than 3 mg/L)
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