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EC number: 274-230-7 | CAS number: 69943-66-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study conducted according to internationally accepted testing guideline.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Trisodium [3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-2-hydroxy-5-nitrobenzenesulphonato(3-)][2-hydroxy-3-[(2-hydroxy-1-naphthyl)azo]-5-nitrobenzenesulphonato(3-)]chromate(3-)
- EC Number:
- 274-230-7
- EC Name:
- Trisodium [3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-2-hydroxy-5-nitrobenzenesulphonato(3-)][2-hydroxy-3-[(2-hydroxy-1-naphthyl)azo]-5-nitrobenzenesulphonato(3-)]chromate(3-)
- Cas Number:
- 69943-66-4
- Molecular formula:
- C32H18CrN8O14S2Na3
- IUPAC Name:
- Trisodium [3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H pyrazol-4-yl)azo]-2-hydroxy-5-nitrobenzenesulphonato(3-)][2hydroxy-3-[(2-hydroxy-1-naphthyl)azo]-5-nitrobenzenesulphonato(3-)]chromate(3-)
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Velaz Prague.
- Weight at study initiation: about 150 g.
- Housing: semparated, in plastic polypropylene cages T4 (supplied by s.p. Velaz Prague).
- Diet: animals were fed with commercial granular food mixture Altromin 1320, supplied by s.p. Velaz Prague. Daily dose of 15 g/animal/day.
- Fasting period before study: the day before of the test, animals were not fed.
- Water: CSN 757111 ad libitum.
- Bedding: wood shavings, from light wood.
- Acclimation period: 1 week.
Cleaning and disinfection of premises menagerie were made at dates determined, according to the standard operating procedures and compliance regime measures.
ENVIRONMENTAL CONDITIONS
- Temperature: controlled temperature at 22 ± 3 °C.
- Humidity: 50 ± 15 %.
- Photoperiod: 12 hrs dark / 12 hrs light, by fluorescent lamp.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: 20 % - Doses:
- 3981, 5020, 6310, 7943, 10000 mg/kg
- No. of animals per sex per dose:
- 10 rats x dose
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: immediately after application, ca 30 minutes, after 3 hours post-application. The day after the application the observations were made in the morning and in the afternoon, while in the following days at least once daily. Body weight was measured at the beginning and end of the experiment.
- Necropsy of survivors performed: yes; internal organs were assessed for colour, size, consistency and structure.
- Other examinations performed: appearance of the skin, hair, visible mucous membranes status, mental activity, somatomotor activity, reactivity to stimulus, lacrimation, respiration, digestion, urogenital and circulatory apparatus. Organs and muscles were examined macroscopically. If post-mortem the bladder of animals was full, the urines were analyzed focusing on the detection of proteins, blood sugar, ketones, bilirubin, urobilinogen and pH. - Statistics:
- The LD50 is calculated according to the probit method by Blisse.
Data on mortality, the frequency and using the logarithmic dose levels used, were inserted into a computer and analyzed by the PROBIT program, from October 1991 VUOS.
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- ca. 6 620.9 mg/kg bw
- Based on:
- test mat.
- Mortality:
- 3981 mg/kg: 0.0 %
5020 mg/kg: 30.0 %
6310 mg/kg: 30.0 %
7943 mg/kg: 70.0 %
10000 mg/kg: 100.0 % - Clinical signs:
- other: After 3 hours post-application at dose level of 10.00 g/kg the following symptoms were recorded: - the appearance of skin and hair: stained with excretions. - nutritional status: cachectic. - appearance of visible mucous membranes: in the range of physiol
- Other findings:
- Autopsies of dead rats administered at dose of 1000 mg/kg revealed:
- External examination of the cadaver
Appearance of hair, skin: hair contaminated with excretions.
Nutritional status: cachectic.
Natural body openings: no macroscopical, patomorphological changes.
Integrity of the body surface: intect.
Postmortality: rigor mortis.
- Organs of head and neck: no macroscopical, patomorphological changes.
- Internal inspection cadaver
Tongue, esophagus, trachea: no macroscopical, patomorphological changes.
Lungs: pink, spongy consistency, airy, without macroscopical, patomorphological changes.
Heart: brownish-red colour, stiffer consistency, without macroscopical, patomorphological changes.
- Abdominal organs
Stomach: acute dilatation, filled with mash content, wall colour brown.
Guts: filled with sparse content, brown-coloured wall
Liver: brownish-red color, stiffer consistency, without macroscopical, patomorphological changes.
Spleen: stiffer consistency, red colour, without macroscopical, patomorphological changes.
Kidney: brownish-red colour, stiffer consistency, without macroscopical, patomorphological changes.
Peritoneum: no macroscopical, patomorphological changes.
Bladder: empty, without macroscopical, patomorphological changes.
- Urine - biochemical tests not established
Symptoms recorded at the lower dose level administered, are analogous to those of the maximum dose.
Any other information on results incl. tables
Dose mg/kg |
Percent mortality | |||||||||||||
Day 1 | Day 2 | Day 3 | Day 4 | Day 5 | Day 6 | Day 7 | Day 8 | Day 9 | Day 10 | Day 11 | Day 12 | Day 13 | Day 14 | |
1/2 hr | 3 hrs | |||||||||||||
3981 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
5020 | 0 | 30 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
6310 | 0 | 20 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
7943 | 0 | 70 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
10000 | 0 | 70 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Dose mg/kg |
Number of dead animals |
Percentage | |
Males | Females | ||
3981 | 0 | 0 | 0 |
5020 | 1 | 2 | 30 |
6310 | 0 | 3 | 30 |
7943 | 4 | 3 | 70 |
10000 | 5 | 5 | 100 |
Body weight
Dose mg/kg |
Males g/kg |
Females g/kg |
||||
Start test | End test | Difference | Start test | End test | Difference | |
3981 | 166.4 | 173 | + 6.6 | 160 | 168 | + 8 |
5020 | 167.6 | 178.8 | + 11.22 | 154.4 | 158.8 | + 44.4 |
6310 | 160.8 | 171.6 | + 10.8 | 159.6 | 154.8 | - 4.8 |
7943 | 156 | 148.8 | - 7.2 | 163.2 | 166 | - 3.2 |
1000 | 162.8 | 163.2 | - 9.6 | 148.8 | 148.4 | - 0.4 |
Results
Dose g/kg |
Group | Percentage of mortality | Log. Dose | Probits Exp. |
3981 | 10 | 0.0 | 0.59999 | |
5020 | 10 | 30.0 | 0.70070 | 4.476 |
6310 | 10 | 30.0 | 0.80003 | 4.476 |
7943 | 10 | 70.0 | 0.89998 | 5.524 |
10000 | 10 | 100.0 | 1.00000 |
Applicant's summary and conclusion
- Interpretation of results:
- not classified
- Remarks:
- Migrated information according to the CLP Regulation (EC 1272/2008) Criteria used for interpretation of results: EU
- Conclusions:
- Oral LD50: 6620.9 mg/kg (6098.6 - 7187.9 mg/kg).
- Executive summary:
The acute toxicological characterization of the substance was determined by Acute toxicity test, according to the OECD guideline 401.
Groups of ten rats (5 males and 5 females) were administrated by oral gavage with 3981, 5020, 6310, 7943, 10000 mg/kg of test material. The LD50 was calculated according to the probit method by Blisse.
Conclusion
The LD50 was calculated to be 6.6209 g/kg (6.0986 - 7.1879 g/kg).
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