Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 202-860-4 | CAS number: 100-52-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to reproduction: other studies
Administrative data
- Endpoint:
- toxicity to reproduction: other studies
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- Although the study by Watanuki and Sakaguchi (1981) is in an in vitro system, the cells used were Fischer rat embryo fibroblasts. The lack of effect on a cell line with embryonic origin provides support that benzaldehyde does not impact normal cellular function of that embryonic cell line.
Data source
Reference
- Reference Type:
- publication
- Title:
- Glucose Dependent Growth Inhibition of SV40 Transformed Rat Cells with Benzaldehyde
- Author:
- Watanuki M, Sakaguchi K
- Year:
- 1 981
- Bibliographic source:
- Agric Biol Chem 45 (1): 319-21
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Experiment 1. - Sugar-dependent Cell Growth Inhibition by Benzaldehyde:
Normal and SV40-transformed cells plated at 0.5 x 10^5 cells/35 mm-dish (Falcon) in 2 ml of Eagle's minimum essential medium containing 10% newborn calf serum and antibiotics (100 units penicillin, 100 microg streptomycin, and 60 microg kanamycin per ml of medium) were allowed to grow for I day at 37° in a CO2 incubator (95% air and 5% CO2), On day 2, the culture medium was replaced with 2ml of glucose free culture medium of which newborn calf serum was dialized against 4-fold volume of phosphate buffer saline 5 times, which contained 5.6mM glucose (A), 5.6mM mannose (B) or 5.6mM fructose (C). One half of the group was treated with benzaldehyde (50 microg/ml) and another half was for control. On day 4, determinations of viable cell number were performed in a haemacytometer in the presence of trypan blue.
Experiment 2. - Inhibition of Cell Growth by Benzaldehyde in Culture Media with Various Energy Sources:
Experimental procedures were almost the same as those described in Experiment 1 except that cells were counted on day 3. The cells were treated with benzaldehyde (50 microg/ml) in glucose- and glutamine-free culture medium supplemented with 5.6 mM glucose and 2 mM glutamine (A), 8 mM glutamine (B) and 50mM glycerol (C) as the energy source(s) for cultured cells.
Experiment 3. - Glucose Dependent Inhibition by Benzaldehyde of Glucose Incorporation into SV40-transformed Cells:
SV 40-transformed cells plated at I x 10^5 cells/35 mm dish were cultured for 2 days. After 2 washes ofmonolayer cells with Hanks' BSS, pH 7.4, the cells were preincubated in 2 ml of Hanks' BSS for 30 min at 37° in a CO2 incubator. Incubation was initiated by replacing the incubation medium with 1 ml of Hanks' BSS containing bezaldehyde (50 microg/ml) or H20 (for control) and [14C]D-glucose (1 microCi/ml) diluted with the desired concentrations of D-glucose. After 30 min, incubation was stopped by chilling the plate on ice, followed by washing the cells 3 times with 2 ml of cold PBS, pH 7.3. The cells were scraped with a rubber policeman into I ml of cold PBS. The scraped cells were transferred onto Whatman GF/C glass microfibre filter with another 2 ml of cold PBS. The filters were dried, and radioactivities were determined with 10 ml of a toluene scintillator solution. - GLP compliance:
- not specified
- Type of method:
- in vitro
Test material
- Reference substance name:
- Benzaldehyde
- EC Number:
- 202-860-4
- EC Name:
- Benzaldehyde
- Cas Number:
- 100-52-7
- Molecular formula:
- C7H6O
- IUPAC Name:
- benzaldehyde
- Details on test material:
- No data given
Constituent 1
Results and discussion
Observed effects
When compared to SV-40 transformed cells, the growth of normal rat embryo fibroblasts was not inhibited by 50 microg/ml benzaldehyde in culture. This was true for all three sugar sources evaluated (glucose, mannose and fructose).
Experiment 2. - Inhibition of Cell Growth by Benzaldehyde in Culture Media with Various Energy Sources:
In this experiment, non-sugar energy sources were evaluated. Similarly, the growth of normal rat embryo fibroblasts was not inhibited by 50 microg/ml benzaldehyde in the presence of 8 mM glutamine or 50 mM glycerol.
Experiment 3. - Glucose Dependent Inhibition by Benzaldehyde of Glucose Incorporation into SV40-transformed Cells:
Where benzaldehyde did inhibit the uptake of glucose in the SV-40 transformed cells, no effect of benzaldehyde on the incorporation of glucose was observed for normal rat embryo fibroblasts.
Applicant's summary and conclusion
- Conclusions:
- Benzaldehyde did not exhibit effects on normal rat embryo fibroblasts, but did affect SV-40 transformed rat embryo fibroblasts.
- Executive summary:
Although this is an in vitro study, it demonstrates that benzaldehyde does not affect normal rat embryo fibroblasts in culture. The relevance of this model to the intact animal is not clear, however, it does provide Weight of Evidence support that benzaldehyde may not have effects on developing embryonic tissues.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.