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EC number: 269-085-1 | CAS number: 68187-30-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study with acceptable restrictions (temperature slightly higher (0.3 °C) then recommended in guideline: as no significant immobility occurred this deviation is considered as uncritical)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Samples were taken from the control and each test concentration after 0, 24 and 48 h.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A stock solution containing 100 mg test substance/L was prepraed in water. The stock solution was then diluted to prepare the concentrations to be tested. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain: Berlin
- Source: Umweltbundesamt Berlin
- Age at study initiation: < 24 h
- Method of breeding: similar to the method described in ISO guideline 6341
- Feeding during test: none
ACCLIMATION
- Acclimation period: 24 hours
- Acclimation conditions: same as test
- Type and amount of food: unicellular green algae
- Feeding frequency: twice a week - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 250 mg/L as CaCO3
- Test temperature:
- 21.2 - 22.0 °C
- pH:
- 7.4 - 7.9
- Dissolved oxygen:
- 8.1 - 8.8 mg/L
- Nominal and measured concentrations:
- Nominal concentrations: 2.2, 4.6, 10, 22, 46 and 100 mg/L
Measured concentrations (geom. mean): 1.7, 3.9, 9.1, 18.7, 37 and 77.1 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: beaker
- Material: glass; Size: 50 mL; Fill volume: 20 mL
- Renewal rate of test solution (frequency/flow rate): every 24 h
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionized water
- TOC content: < 2 mg/L
- Particulates: < 20 mg/L
- Culture medium different from test medium: no (Elendt M4)
- Intervals of water quality measurement: The pH and dissolved oxygen concentration were measured at the beginning and at the end of the test and at every medium renewal in the old and the new test solution.
EFFECT PARAMETERS MEASURED: Immobilization was recorded after 24 and 48 h test duration. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 49 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 77 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- In the control, none of the daphnia died or showed any signs of abnormal behaviour throughout the test.
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- EC50 (24h) = 1.4 mg/L
- Other: The EC50 (24h) for the reference substance was determined to be in the accepted range of 0.6 - 1.7 mg/L. - Validity criteria fulfilled:
- yes
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- supporting study
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 49 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
Referenceopen allclose all
Analytical determination:
The concentration of the test item was calculated through measured DOC divided by the content of organic carbon (60%) of the test item. The measured concentrations showed good correlation with the nominal concentrations. The geometric means of the measured concentrations were in a range of 77 - 91 % of the nominal concentrations. Therefore, the geometric mean of the measured concentrations was used for the determination of the results. The measured DOC values in the control and in the lowest concentrated treatment were in a similar range. The measured DOC concentration in the control at t = 0 h was unexpectedly high. This might be caused by a contaminated test vessel.
Description of key information
In a final decision on a compliance check ECHA requested the registrant to conduct a long-term toxicity testing on aquatic invertebrates with CAS 68187-30-4. According to the decision, to Annex VII, Section 9.1.1, Column 2 of REACH and to prevent unnecessary animal testing, a short-term toxicity study on aquatic invertebrates does not need to be provided.
Key value for chemical safety assessment
Additional information
No studies investigating the short-term toxicity of l-Glutamic acid, N-coco acyl derivs., disodium salts (CAS No. 68187-30-4) to aquatic invertebrates are available. Thus, available data for the source substance l-Glutamic acid, N-coco acyl derivs., monosodium salts (CAS No. 68187-32-6) was used in a read across approach. The read across is considered valid as both substances are structurally identical (solely differing in the degree of deprotonation being di- and monosodium salts, respectively), which assume very similar physico-chemical properties resulting in identical environmental behavior and ecotoxicity.
The read across key study was performed according to GLP and OECD guideline 202 using Daphnia magna as test organism (Laus, 2011). Nominal concentrations between 2.2 and 100 mg/L were tested over a period of 48 h. Thus, a 48h-EC50 is determined to be 49 mg/L based on the measured test concentration. Due to the very high structural similarity, this result can also be considered for l-Glutamic acid, N-coco acyl derivs., disodium salts (CAS No. 68187-30-4).
In a final decision on a compliance check ECHA requested the registrant to conduct a long-term toxicity testing on aquatic invertebrates with CAS 68187-30-4. According to the decision, to Annex VII, Section 9.1.1, Column 2 of REACH and to prevent unnecessary animal testing, a short-term toxicity study on aquatic invertebrates does not need to be provided.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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