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EC number: 217-204-2 | CAS number: 1774-47-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Well documented study according to international accepted guidelines and GLP compliant.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- Trimethyloxosulphonium iodide
- EC Number:
- 217-204-2
- EC Name:
- Trimethyloxosulphonium iodide
- Cas Number:
- 1774-47-6
- Molecular formula:
- C3H9OS.I
- IUPAC Name:
- trimethyl(oxo)-λ⁶-sulfanylium iodide
- Test material form:
- solid: crystalline
- Details on test material:
- Test item: Trimethylsulfoxonium Iodide (CAS 1774-47-6)
Batch No.: 20130228 (0056D53)
Physical state: solid, crystalline powder
Colour: white or light-yellow
Content (Chemical Titration): 99.66 %
Storage: on cool and dry place, protected from light
Constituent 1
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- CBA
- Sex:
- female
- Details on test animals and environmental conditions:
- Species and strain: CBA/Ca mice
Source: TOXI-COOP ZRT.
Hygienic level at arrival: SPF
Hygienic level during the study: Good conventional
Number of animals: 28 animals/main test (4 animals/treatment group)
Housing during the test: Grouped caging (4 animals/cage)
Sex: Female, nulliparous, non-pregnant
Age of animals: Young adult mice;
9-12 weeks old (at start of the main test)
Body weight range at starting: 17.4-23.6 g
Light: 12 hours daily, from 6.00 a.m. to 6.00 p.m.
Temperature: 22 ± 3 °C
Relative humidity: 30 – 70 %
Study design: in vivo (LLNA)
- Vehicle:
- dimethyl sulphoxide
- Remarks:
- AOO (acetone:olive oil, 4:1) for positive control groups
- Concentration:
- The test item was administered at four different concentrations (10; 5; 2.5; 1 w/v%) according to the results of the dose range finding test.
- No. of animals per dose:
- 4 animal/dose
- Details on study design:
- Selection of test item concentrations was based on the results of a formulation evaluation and also results of preliminary irritation/toxicity tests to find the best solvent and the maximum applicable concentration according to the relevant guidelines [1-2]. Based on the preliminary test results the test item was examined in the main test at 10 %, 5 %, 2.5 % and 1 % (w/v) concentrations as formulations in the selected vehicle of Dimethyl sulfoxide (DMSO). Appropriate positive control (α-Hexylcinnamaldehyde [HCA] in 25 % (w/v) concentration), furthermore two negative control groups dosed with the vehicles of the test and positive control groups, respectively, were employed. The positive control item (25 % (w/v) HCA in Acetone: Olive oil 4:1 (v/v) mixture, AOO) induced the appropriate stimulation over the control, thus confirming the validity of the assay.
- Positive control substance(s):
- hexyl cinnamic aldehyde (CAS No 101-86-0)
Results and discussion
- Positive control results:
- The positive control group animals were treated with 25 % (w/v) HCA solution (dissolved in AOO) concurrent to the test item groups. No mortality, cutaneous reactions or signs of toxicity were observed in the positive control group.
Significant lymphoproliferative response (SI ≥ 3) was noted for HCA (SI = 6.3). The results of the positive control item demonstrated appropriate performance of the test in accordance with the relevant guidelines and confirmed validity of the assay.
In vivo (LLNA)
Resultsopen allclose all
- Parameter:
- SI
- Remarks on result:
- other: see Remark
- Remarks:
- The stimulation index (SI = the DPN of a treated (positive control or test item) group divided by the DPN of the respective negative control group) for each treatment group was also calculated. A stimulation index of 3 or greater is an indication of a positive result. Based on the results EC3 value (dose calculated to induce a stimulation index of 3) of the test item was not calculated. Dose-response relationship was evaluated by linear regression. All calculations were made by Microsoft Excel Software. (See Table1 fo the values.)
- Parameter:
- other: disintegrations per minute (DPM)
- Remarks on result:
- other: see Remark
- Remarks:
- DPM (disintegration per minute) was measured for each treatment group. The measured DPM values were corrected with the background DPM value. The average of the two measured DPM values of 5 % (w/v) TCA solutions was used as the background DPM value. The results were expressed as DPN (DPM divided by the number of pooled lymph nodes). (See Table1 fo the values.)
Any other information on results incl. tables
Test Group Name |
Measured DPM/group |
Group* DPM |
DPN (DPN/Node) |
Stimulation Index Values |
Vehicle control for the positive control: AOO |
26248 |
26227.5 |
3278.4 |
1.0 |
Positivecontrol: |
166563 |
166542.5 |
20817.8 |
6.3 |
TRIMETHYLSULFOXONIUM IODIDE |
38897 |
38876.5 |
4859.6 |
1.2 |
TRIMETHYLSULFOXONIUM IODIDE |
30479 |
30458.5 |
3807.3 |
0.9 |
TRIMETHYLSULFOXONIUM IODIDE |
35648 |
35627.5 |
4453.4 |
1.1 |
TRIMETHYLSULFOXONIUM IODIDE |
31539 |
31518.5 |
3939.8 |
1.0 |
Vehicle control for the test item: DMSO |
33046 |
33025.5 |
4128.2 |
1.0 |
*Group DPM = measured DPM(group)- average DPM(background)
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Remarks:
- Migrated information
- Conclusions:
- Under the conditions of the present Local Lymph Node Assay, TRIMETHYLSULFOXONIUM IODIDE (CAS No. 1774-47-6) tested at the maximum attainable concentration of 10 % and at concentrations of 5 %, 2.5 % and 1 % (w/v) as formulations in an appropriate vehicle (DMSO) was shown to have no skin sensitization potential.
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