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Diss Factsheets
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EC number: 482-430-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- February 14th - 16th 2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study conducted in accordance with GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 006
- Report date:
- 2006
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- other: EEC C2: Acute toxicity to Daphnia OECD 202: Acute immobilisation test
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Test material form:
- other: Liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Samples of the test solutions in which the organisms were exposed, were taken for analytical verification at the start of the definitive study, before and after renewal of solutions at 24 hours and at the end of the 48 hour exposure period. Samples were frozen at -22ºC until test substance specific analysis of each test concentration was carried out.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Culture derived from IRCHA type A (clone5)
Source: Shell Research Laboratories
Age of Daphnia at start of test: Less than 24 hours
Study design
- Test type:
- semi-static
- Water media type:
- not specified
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 156 mg CaCO3/L
- Test temperature:
- 20±1 ºC
- pH:
- 7.8±0.2
- Dissolved oxygen:
- 60% minimum
- Nominal and measured concentrations:
- The definitive test concentrations were prepared as; 0 (control), 5, 10, 20, 40, 80 and 160 mg/l
- Details on test conditions:
- Preparation of test solutions
A 160 mg/l stock solution of Inhibitor AHM P500 was prepared in dilution water. The test concentrations were prepared by addition of the appropriate volume of the stock solution to the dilution water to give the required concentrations for the test.
The definitive test concentrations were prepared as; 0 (control), 5, 10, 20, 40, 80 and 160 mg/l
Definitive toxicity procedure
Five Daphnia were transferred into four replicate 50 ml dishes containing 25ml of dilution water. The test vessels were placed into an incubator at 21 ±1 ºC with a light cycle of sixteen hours light and eight hours dark. To limit aerial contamination of the test vessels and reduce evaporative loss, a transparent perspex sheet was placed over the tops of the dishes. - Reference substance (positive control):
- yes
- Remarks:
- Water
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 75 mg/L
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 19 mg/L
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 80 mg/L
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 10 mg/L
- Details on results:
- Behavioural abnormalities: At 24 hrs some daphnids showed slower swimming at a concentration of 40 mg/L, at 48 hrs all remaining daphnids showed slower swimming at 20 and 40 mg/L.
- Mortality of control: No immobilised daphnids were observed in the control.
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None- Results with reference substance (positive control):
- Results with reference substance valid? Yes
- EC50/LC50 of reference subtstance potassium dichromate: 0.36 mg/L
- Other:
Any other information on results incl. tables
The number of Daphnids immobilised and the cumulative % immobilisation are presented in Table 1.
Nominal Concentration mg/L |
Number immobilised |
% immobilisation |
||
24 hours |
48 hours |
24 hours |
48 hours |
|
0 |
0 |
0 |
0 |
0 |
5 |
0 |
0 |
0 |
0 |
10 |
0 |
0 |
0 |
0 |
20 |
2 |
13 |
10 |
65 |
40 |
4 |
19 |
20 |
95 |
80 |
11 |
20 |
55 |
100 |
160 |
16 |
20 |
80 |
100 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48-hour EC50 value of inhibitor AHM P500 to Daphnia magna was estimated to be 19 mg/l. The highest no observed effect concentration (NOEC) after 48 hours was 10 mg/l. The lowest test concentration that immobilised all twenty Daphnia within 48 hours was 80 mg/l
All Daphnia that were noted immobile at 24 and 48 hours were subjected to careful observation and were judged as dead. No Daphnia were observed alive but immobile. No other adverse effects on the organisms were noted. - Executive summary:
A study was performed in accordance with OECD 202 and to GLP, to determine the effects of Inhibitor AHM P500 to Daphnia magna.
The study was performed under semi-static conditions at test concentrations of 0, 5, 10, 20, 40, 80 and 160 mg/L defined in a preliminary test.
Potassium dichromate was employed as the reference substance.
All Daphnia that were noted immobile at 24 and 48 hours were subjected to careful observation and were judged as dead. No Daphnia were observed alive but immobile.
Validity criteria for the reference substance and blank (0 mg/L) were considered met.
The 48-hour EC50 value of Inhibitor AHM P500 to Daphnia magna was estimated to be 19 mg/l. The highest no observed effect concentration (NOEC) after 48 hours was 10 mg/l.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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