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Environmental fate & pathways

Adsorption / desorption

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Reference
Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From Jan. 08, 2003 to Feb. 14, 2003
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
Deviations:
no
Qualifier:
according to guideline
Guideline:
OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Type of method:
HPLC estimation method
Media:
soil/sewage sludge
Radiolabelling:
no
Test temperature:
25 °C
Details on study design: HPLC method:
EQUIPMENT
- Apparatus: HPLC pump G1311A, autosampler G1313A, column oven G1316A, degaser (G1322A)
- Type, material and dimension of analytical (guard) column: Stationary phase
- Detection system: UV/VlS detector G1314A by Agilent
- Flow rate: 1 mL/min

MOBILE PHASES
- Type: acetonitrile / cleaned water, isocratic elution: 60 : 40 % (v/v)
- Experiments with additives carried out on separate columns: yes


DETERMINATION OF DEAD TIME
- Method: The dead time was determined by using a "dead time marker". Dead time markers are substances which are not retarded from column or mobile phases.

REFERENCE SUBSTANCES
- Identity: Benzamide, 2-Nitrobenzamide, N, N-Dimethylbenzamide, Manuran, Triadimenol, Linuron, 1,2,3-Trichlorobenzene, Pyrazophos, Diclofop-methyl

DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column: 0.31 µg
- Quantity of reference substances: 0.92-1.27 µg
- For calibration the retention times of a series of substances with known adsorption coefficients were measured and a calibration graph was established

REPETITIONS
- Number of determinations: Two

EVALUATION
- Calculation of capacity factors k': Yes
- Calculation of retention times: Yes
- Determination of the log Koc value: Yes
Computational methods:
Not applicable
Type:
log Koc
Value:
4.36 dimensionless
Temp.:
25 °C
Remarks on result:
other: extrapolated
Key result
Type:
log Koc
Value:
> 4.2 dimensionless
Temp.:
25 °C
Remarks on result:
other: ( 25 °C is column temperature)
Details on results (HPLC method):
- Retention times of reference substances used for calibration: 6.86- 6.87 min
- Details of fitted regression line (log k' vs. log Koc): Measurement 1 & 2: 0.09 vs. 4.36;
Transformation products:
no

Table 1. log Koc of the test substance.

 

tR in min

K

Log k

log koc

Measurement 1

6.87

1.24

0.09

4.36

Measurement 2

6.86

1.23

0.09

4.36
Validity criteria fulfilled:
yes
Conclusions:
Under the test conditions, the adsorption coefficient (log Koc) of the test substance was determined to be >4.2
Executive summary:

A study was performed to determine the adsorption coefficient (log Koc) of the test substance according to EU Method C.19. and OECD Guideline 121 in compliance with GLP.

Test was conducted using HPLC method. The dead time was determined by using a "dead time marker". For calibration the retention times of a series of substances with known adsorption coefficients were measured and a calibration graph was established. The adsorption coefficient of the test substance was derived from its retention time and the calibration graph. The calibration substances were injected in two series and one before and one after the injection of the test substance.

The dead time was determined to be 3.07 min and log Koc of the test substance at two measurements was 4.36.

Under the test conditions, the adsorption coefficient (log Koc) of the test substance was determined to be >4.2.

Description of key information

The adsorption coefficient (log Koc) of the test substance was determined to be > 4.2 using HPLC method.

Key value for chemical safety assessment

Koc at 20 °C:
4.4

Additional information

A study was performed to determine the adsorption coefficient (log Koc) of the test substance according to EU Method C.19. and OECD Guideline 121.


Test was conducted using HPLC method. The dead time was determined by using a "dead time marker". For calibration the retention times of a series of substances with known adsorption coefficients were measured and a calibration graph was established. The adsorption coefficient of the test substance was derived from its retention time and the calibration graph. The calibration substances were injected in two series and one before and one after the injection of the test substance.The dead time was determined to be 3.07 min and log Koc of the test substance at two measurements was 4.36. The adsorption coefficient (log Koc) of the test substance was determined to be >4.2, with 4.2 being the adsorption coefficient of the highest calibration standard. (Schminke A, 2003)