Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 222-059-3 | CAS number: 3332-27-2
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
No chronic daphnia toxicity studies have been performed using C14 amine oxide. Data are read across from C12-14 AO on the basis that this substance contains a significant amount of C14 AO and the remainder comprises an analogue substance with very similar chain length.
One long term (21 days) toxicity to Daphnia magna study is available for C12-14 AO. A no effect concentration of 0.7 mg AO/L was reported in the study.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.7 mg/L
Additional information
No chronic daphnia toxicity studies have been performed using C14 amine oxide. Data are read across from C12-14 AO on the basis that this substance contains a significant amount of C14 AO and the remainder comprises an analogue substance with very similar chain length.
The long-term toxicity of C12-14 AO was evaluated in a 21 day survival and reproduction test with Daphnia magna, following the OECD 211 guideline [Maki AW (1977)]. Four replicates per concentration, 5 organisms per replicate, were tested in a flow-through system. Mean measured test concentrations were: 0, 0.08, 0.17, 0.34, 0.70, and 1.35 mg AO/L. Survival was monitored at 24 hours, 96 hours, 7 days, and daily thereafter. Reproduction was monitored beginning with the production of the first brood on day 7 or 8, and daily thereafter. EC50s were calculated by probit analysis. Results are based on the mean measured concentration of the amine oxide.
The 21-day NOEC was 0.70 mg AO/L, based on both survival and reproduction. The 21 day EC50 was 0.96 mg AO/L based on mortality. The 21-day EC50’s, based on total young production, average brood size, and % days reproduction occurred, were 0.88 mg AO/L, 1.01 mg AO/L, and 1.04 mg AO/L, respectively.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
