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EC number: 203-457-6 | CAS number: 107-05-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: - guideline compliant study - GLP compliant
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- 3-chloropropene
- EC Number:
- 203-457-6
- EC Name:
- 3-chloropropene
- Cas Number:
- 107-05-1
- Molecular formula:
- C3H5Cl
- IUPAC Name:
- 3-chloroprop-1-ene
- Details on test material:
- Sponsor's identification: Allyl chloride
Description: clear colourless liquid
Analytical purity: 98.7%
Impurities: water 18 mg/kg
Purity test date: 18 November 2009
Batch number: RBA091118A
Expiry date: 27 November 2011
Stability under test conditions: stable under recommended storage conditions
Date received: 27 November 2009
Storage conditions: approximately 4°C in the dark
Constituent 1
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- other: CBA/CaOlaHsd
- Sex:
- female
- Details on test animals and environmental conditions:
- TEST ANIMALS
- Source: Harlan Laboratories UK Limited, Bicester, Oxon, UK
- Age at study initiation: 8 - 12 wks
- Weight at study initiation: 15 to 23 g
- Housing: individually housed in suspended solid-floor polypropylene cages furnished with softwood woodflakes
- Diet (e.g. ad libitum): ad libitum, 2014 Teklad Global Rodent diet supplied by Harlan Teklad, Blackthorn, Bicester, Oxon, UK
- Water (e.g. ad libitum): ad libitum, tap water
- Acclimation period: > 5 d
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19 to 25
- Humidity (%): 30 to 70
- Air changes (per hr): 15
- Photoperiod (hrs dark / hrs light): 12/12
IN-LIFE DATES: From: 2010-01-13 To: 2010-01-27
Study design: in vivo (LLNA)
- Vehicle:
- acetone/olive oil (4:1 v/v)
- Concentration:
- 25, 50 % (v/v) in olive oil and 100 % (undiluted)
- No. of animals per dose:
- 5
- Details on study design:
- RANGE FINDING TESTS:
- Compound solubility: completely soluble in the vehicle
- Irritation: The preliminary screening test suggested that the test material would not produce systemic toxicity or excessive local irritation at the highest suitable concentration.
- Lymph node proliferation response: not assessed
MAIN STUDY
ANIMAL ASSIGNMENT AND TREATMENT
- Name of test method: random
- Criteria used to consider a positive response: The test material will be regarded as a sensitiser if at least one concentration of the test material results in a threefold or greater increase in 3HTdR incorporation compared to control values.
TREATMENT PREPARATION AND ADMINISTRATION: animals, were treated with 50 pl (25 pl per ear) of the undiluted test material or the test material as a solution in acetone/olive oil 4:1 at concentrations of 50% or 25% v/v. A further group of five animals was treated with acetone/olive oil 4:1 alone. A concurrent positive control test, using a group of five animals, was also performed with the known sensitiser, a-Hexylcinnamaldehyde tech., 85%, at a concentration of 15% v/v in acetone/olive oil 4:1. - Positive control substance(s):
- hexyl cinnamic aldehyde (CAS No 101-86-0)
- Statistics:
- Data was processed to give group mean values for disintegrations per minute and standard deviations where appropriate. Individual and group mean disintegrations per minute values were assessed for dose response relationships by analysis of homogeneity of variance followed by one way analysis of variance (ANOVA). In the event of a significant result from the ANOVA, pairwise comparisons were performed between control and treated groups. For homogenous datasets Dunnett's Multiple Comparison test was used and for non-homogenous datasets Dunnett's T3 Multiple Comparison Method was used.
Results and discussion
- Positive control results:
- a-Hexylcinnamaldehyde tech., 85%, at a concentration of 15% v/v in acetone/olive oil 4:1: stimulation index: 6.21, Positive
In vivo (LLNA)
Resultsopen allclose all
- Parameter:
- SI
- Remarks on result:
- other: < 3 for all treatment groups, > 3 for the positive test substance, see table 1
- Parameter:
- other: disintegrations per minute (DPM)
- Remarks on result:
- other: see table 1
Any other information on results incl. tables
- table 1: Individual Disintegrations per Minute and Stimulation Indices
Treatment Group |
Animal |
dpm/ |
Mean dpm/Animal |
Stimulation |
Result |
Vehicle |
1 -1 |
1668.60 |
1655. 95 |
N/A |
N/A |
1-2 |
1290.06 |
||||
1-3 |
2285.84 |
||||
1-4 |
1769.67 |
||||
1-5 |
1265.60 |
||||
Test Material |
2-1 |
938.63 |
1293.35 |
0.78 |
Negative |
2-2 |
1558.52 |
||||
2-3 |
1699.85 |
||||
2-4 |
1361.70 |
||||
2-5 |
908.03 |
||||
Test Material |
3-1 |
1484.54 |
1246.26 |
0.75 |
Negative |
3-2 |
1252.86 |
||||
3-3 |
1384.65 |
||||
3-4 |
595.10 |
||||
3-5 |
1514.15 |
||||
Test Material |
4-1 |
5363.54 |
3255 21 . (±1380.91) |
1.97 |
Negative |
4-2 |
3626.75 |
||||
4-3 |
1772.00 |
||||
4-4 |
3174.02 |
||||
4-5 |
2339.74 |
||||
Positive Control |
5-1 |
6359.41 |
10278.58* (±3233.93) |
6.21 |
Positive |
5-2 |
14875.75 |
||||
5-3 |
8667.83 |
||||
5-4 |
9670.29 |
||||
5-5 |
11819.63 |
Applicant's summary and conclusion
- Interpretation of results:
- not sensitising
- Remarks:
- Migrated information
- Conclusions:
- 3-chloropropene was tested in a local lymphnode assay according to OECD TG 429.
The test material was considered to be a non-sensitiser under the conditions of the test.
a-Hexylcinnamaldehyde, tech., 85% gave a Stimulation Index of greater than 3 when tested at a concentration of 15% v/v in acetone/olive oil 4:1 - Executive summary:
The present study (Sanders 2010) was performed to assess the skin sensitisation potential of the test material 3 -chloropropene in the CBA/Ca strain mouse following topical application to the dorsal surface of the ear. The method was designed to meet the requirements of the following:
OECD Guideline for the Testing of Chemicals No. 429 "Skin Sensitisation: Local Lymph Node Assay" (adopted 24 April 2002) Method B42 Skin Sensitisation (Local Lymph Node Assay) of Commission Regulation (EC) No. 440/2008 Methods.
Following a preliminary screening test in which no clinical signs of toxicity were noted at a concentration of 100%, this concentration was selected as the highest dose investigated in the main test of the Local Lymph Node Assay. Three groups, each of five animals, were treated with 50 µl (25 µl per ear) of the undiluted test material or the test material as a solution in acetone/olive oil 4:1 at concentrations of 50% or 25% v/v. A further group of five animals was treated with acetone/olive oil 4:1 alone. A concurrent positive control test, using a group of five animals, was also performed with the known sensitiser, a-Hexylcinnamaldehyde tech., 85%, at a concentration of 15% v/v in acetone/olive oil 4:1. Results. The Stimulation Index expressed as the mean radioactive incorporation for each treatment group divided by the mean radioactive incorporation of the vehicle control group are as follows:
Treatment Group
Concentration
Stimulation Index
Result
Test Material
25% v/v in
acetone/olive oil 4:10.78
Negative
50% v/v in
acetone/olive oil 4:10.75
Negative
100%
1.97
Negative
Positive Control Material
15% v/v in
acetone/olive oil 4:16.21
Positive
The test material was considered to be a non-sensitiser under the conditions of the test. a-Hexylcinnamaldehyde, tech., 85% gave a Stimulation Index of greater than 3 when tested at a concentration of 15% v/v in acetone/olive oil 4:1 thereby validating the test system.
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