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EC number: 204-010-8 | CAS number: 112-85-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 01 June - 04 June 1998
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes
- Remarks:
- According to Japanese GLP standard
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: Test water was directly sampled by each vessel.
- Sample storage conditions before analysis: No storage before analysis. - Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Test item was mixed with HCO-40 at 80 °C. Test medium was added to prepare stock solution (250 mg/L) at room temperature.
- Controls: Blank control and vehicle control
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): HCO-40
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): 20000 mg/L in stock solution and 100 mg/L in final test solution - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Strain: ATCC22662
- Source (laboratory, culture collection): Incorporated Administrative Agency, National Institute for Environmental Studies, Tsukuba, Japan
- Age of inoculum (at test initiation): About 72 hours
- Method of cultivation: On a shaker (100 rpm)
ACCLIMATION
- Acclimation period: 3 days
- Culturing media and conditions (same as test or not): Same as test - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Test temperature:
- 22.5 - 23.4 °C
- pH:
- at the initiaion: 7.9 - 8.2
at the end of the study: 9.8 - 10.2 - Nominal and measured concentrations:
- Nominal concentration: 5 mg/L
Measured concentration: 4.59 mg/L at 0 hour and 3.7 mg/L at 72 hour - Details on test conditions:
- TEST SYSTEM
- Test vessel: 300 mL Erlenmeyer flask
- Type (delete if not applicable): closed with air-permeable stopper
- Material, fill volume: Glass, 100 mL
- Initial cells density: 10000 cells/mL
- Control end cells density: 2689200± 78100 cells/mL in control and 2715900 ± 35100 cells/mL in solvent control
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- No. of vessels per vehicle control (replicates): 3
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Culture medium different from test medium: Same as test
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Photoperiod: Continuously
- Light intensity and quality: 4000 - 5000 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: electronic particle counter
TEST CONCENTRATIONS
- Range finding study: Performed
- Test concentrations: 5 mg/L
- Results used to determine the conditions for the definitive study:
Growth rate compared to control was 99% in solvent control and 93% in 5 mg/L.
Growth rate compared to solvent control was 94% in 5 mg/L. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- EC50: 0.41 mg/L - Reported statistics and error estimates:
- Student's t-test, subsequent to F test for homogeneity of variances for NOEC.
- Validity criteria fulfilled:
- yes
Reference
Table 1. Measured concentration
Nominal Conc. (mg/L) |
Measured Concentration (mg/L) (Percent of nominal) |
|
0 Hour |
72 Hour |
|
Control |
<0.06 |
<0.06 |
Solvent control |
<0.06 |
<0.06 |
5 |
4.59 (92) |
3.70 (74) |
The concentration of test substance was not maintained within 20% of nominal concentration. However, the results were calculated based on nominal concentration, since the water solubility of the substance is actually lower than 0.1 mg/L and the solubility of the substance was increased within this test by using a vehicle. Generally, it can be stated that no effects could be observed within the range of water solubility.
Table 2. Percent growth inhibition of Selenastrum capricornutum
Nominal Concentration (mg/L) |
Biomass |
Growth rate (24-72 h) |
Inhibition (%) |
Inhibition (%) |
|
Control |
- |
- |
Solv. Cont |
- |
- |
5 |
- 12.7 |
- 1.7 |
Description of key information
No toxicity effects up to the limit of water solubility
Key value for chemical safety assessment
Additional information
One study is available on the toxicity of docosanoic acid (CAS 112-85-6) on algae.
The 72 h test was conducted according to OECD 201 and in accordance with GLP requirements. Algae (Selenastrum capricornutum) were exposed to docosanoic acid under static conditions in a limit test by using a solvent. The ErC50 (72 h) and the NOEC for growth rate were determined to be > 5.0 mg/L and ≥ 5.0 mg/L, respectively, based on nominal concentrations. Based on measured concentrations for the test concentration 5 mg/L a value of 4.59 mg/L was determined at the start of the exposure and 3.7 mg/L after 72 h. Thus no effects could be observed within the range of water solubility, which is determined to be 0.016 mg/L.
Based on the results of this study it can be concluded that no toxic effects of docosanoic acid on algae are expected up to the limit of water solubility.
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