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EC number: 215-676-4 | CAS number: 1341-49-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 18th October to 2nd November 1989
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Proprietary guideline compliant study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- GLP compliance:
- not specified
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Ammonium hydrogendifluoride
- EC Number:
- 215-676-4
- EC Name:
- Ammonium hydrogendifluoride
- Cas Number:
- 1341-49-7
- Molecular formula:
- F2H5N
- IUPAC Name:
- ammonium fluoride hydrofluoride
- Details on test material:
- Ammoniumhydrogenfluoride (NH4FxHF), a white crystalline industrial compound. Batch number: 21-06-89.
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- Male and female Sprague-Dawley (SPF outbred) rats, aged 5-6 weeks on arrival, obtained from the Central Institute of Laboratory Animal Breeding, Hannover. Initial body weights for males were 146-28 g, and for females were 118-176 g. Rats were housed individually in stainless steel cages in quarantine at a temperature of 20-22°C and relative humidity of 50-60%, on a 12 hour light/dark cycle. There were approximately 15 air changes per hour. Food (Altromin Co. R1324 mixed food) and tap water were provided ad libitum.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: 2% Tylose
- Details on oral exposure:
- Ammoniumhydrogenfluoride was suspended in 2% Tylose (pH ranging fom 3.9 - 6.8). The substance was administered in a single dose via gastric tube. A fixed dosing volume of 10 ml/kg bw was used.
- Doses:
- 0, 21.5, 46.4, 100 and 147 mg/kg bw.
- No. of animals per sex per dose:
- 10 rats of each sex per dose.
- Control animals:
- yes
- Details on study design:
- The rats were randomised by help of a computer card. The rats were fasted approximately 16-20 hours before dosing. After administration of the single dose, rats were observed for 14 days. Behaviour, food consumption and body weight gain were recorded. At the end of the observation period all surviving animals were sacrificed, necropsied and examined macroscopically. Necropsy was performed immediately after death in rats that died during the observation period.
- Statistics:
- Body weight and food consumption data were evaluated using descriptive methods and ANOVA.
Results and discussion
- Preliminary study:
- Not applicable.
Effect levelsopen allclose all
- Sex:
- male
- Dose descriptor:
- LDLo
- Effect level:
- 100 mg/kg bw
- Remarks on result:
- other: 1 male out of 10 died
- Sex:
- female
- Dose descriptor:
- LDLo
- Effect level:
- 147 mg/kg bw
- Remarks on result:
- other: 8/10 animals died
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 130 mg/kg bw
- Remarks on result:
- other: Estimated based on deaths occurring at dose levels of 100 and 147 mg/kg
- Mortality:
- Six males and 8 females in the high dose group (147 mg/kg bw) within 2 days of administration. One male in the 100 mg/kg bw group died 4.5 hours after administration.
- Clinical signs:
- other: Clinical signs were apparent at doses of 46.4 mg/kg bw and higher and included; dyspnoea, decreased spontaneous motility, sedation, decreased cutaneous circulation, piloerection, ataxia, decreased body tone, respiratory sounds and markedly diminised food
- Gross pathology:
- There were no abnormal findings in rats administered 0, 21.5 or 46.4 mg/kg bw.
1 male in the 100 mg/kg group exhibited a reddened thymus; stomach: gastric dilation, hyperaemia, edematous wall, single ulcera spread over the entire mucosa, suspicion of mucosa detachment, jejunum: single reddened areas, ileum: reddened.
At 147 mg/kg bw: 3 males and 3 females: stomach: gastric dilation, hyperaemia, edematous wall, single ulcera spread over the entire mucosa, suspicion of mucosa detachment. 1 male stomach had gastric mucosa without abnormal finding but some bloody content. 3 males and 3 females displayed bloody content and hyperaemic mucosa of the small intestine. 2 males and 3 females had hyperaemic mucosa in the caecum. 1 male had reddened peyer's patches. 2 males had reddened Nll. mesenteriales. 1 male exhibited grey kidneys, with a white-red coloured cortex, about 2-3 mm spongioid consistency. - Other findings:
- No other findings reported.
Any other information on results incl. tables
Table 1. Toxicity symptoms and time of onset.
Dose group |
Symptoms |
Time of Onset |
Controls |
No abnormal findings |
- |
21.5 mg/kg |
No abnormal findings |
- |
46.4 mg/kg |
1 male and some females showed decreased spontaneous motility, the 1 male also showed dyspnoea. |
Symptoms appeared within 15 minutes of administration, but disappeared 75 minutes after dosing. |
100 mg/kg |
Dyspnoea, respiratory sounds, piloerection, decreased cutaneous circulation, slight ataxia, half closed eyes, decreased spontaneous motility. Sedation was observed although the animals responded to disturbance. 1 male died after 4.5 hours |
Symptoms appeared within 15 minutes of administration, but disappeared 4 days after dosing. |
147 mg/kg |
6/10 males and 8/10 females died within 2 days of administration. Severe dyspnoea, decreased cutaneous circulation, slight ataxia, decreased body tone, prone position, sedation, cyanosis, salivation, lacrimation, mydriasis, slight haemorrhagic discharge from the nose. After 24 hours all surviving rats showed bad general condition. |
Symptoms appeared 10 minutes after dosing and remained until the end of the observation period. |
Applicant's summary and conclusion
- Interpretation of results:
- toxic
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- Ammoniumhydrogenfluoride was classified as Toxic if swallowed according to EC guideline 79/831, annex VI, part 1.
- Executive summary:
The acute oral toxicity of ammonium hydrogenfluoride was determined according to OECD method 401. The test substance was administered to male and female Sprague-Dawley rats by gavage in single doses of 21.5, 46.4, 100 or 147 mg/kg bw. The control group received vehicle alone (2% Tylose). Dose-dependent symptoms occurred immediately after dosing and lasted less than 2 hours after treatment with 46.5 mg/kg bw, or up to 14 days after treatment with 147 mg/kg bw. Symptoms included dyspnoea, ataxia, reduced circulation and decreased spontaneous motility. One male rat (n = 10) died in the 100 mg/kg bw group, and 6 males (n = 10) and 8 females (n = 10) died in the 147 mg/kg bw group. Deaths occurred between 1 hour and 2 days following administration. The LD50 was therefore estimated to be 130 mg/kg bw.
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