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EC number: 251-118-6 | CAS number: 32588-76-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Bioaccumulation: aquatic / sediment
Administrative data
- Endpoint:
- bioaccumulation in aquatic species: fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Aug 1981 - Jan 1982
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- The study was performed by an experienced laboratory using the commercial product in manufacture at the time. The results are consistent with the predicted lack of accumulation based on octanol solubility, molecular weight, size and shape (highly folded) and negligible water solubility. The study was performed at water concentrations higher than its estimated solubility, and whether the analytical method detected the EBTBP molecule or some impurity in the commercial product can be questioned. EBTBP presents a substantial analytical challlenge given its extremely poor solubility in water, organic solvents, and lipid. The results clearly show lack of bioaccumulation.
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 982
- Reference Type:
- publication
- Title:
- A comparison of the fish bioconcentration factors for brominated flame retardants with their nonbrominated analogues.
- Author:
- Hardy et al.
- Year:
- 2 004
- Bibliographic source:
- Envion Toxicol Chem 23(3):656-61.
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: In accordance with the "Law Concerning the Examination and Regulation of Manufacture, etc., of Chemical Substances" (Japan 1973. Law No. 117)
- Principles of method if other than guideline:
- Fish exposed to test substance via water. Water and fish sampled at intervals, and test substance concentrations determined. BCF calculated as ratio of test concentration in fish to water.
- GLP compliance:
- yes
Test material
- Reference substance name:
- N,N'-ethylenebis(3,4,5,6-tetrabromophthalimide)
- EC Number:
- 251-118-6
- EC Name:
- N,N'-ethylenebis(3,4,5,6-tetrabromophthalimide)
- Cas Number:
- 32588-76-4
- Molecular formula:
- C18H4Br8N2O4
- IUPAC Name:
- N,N'-ethylenebis(3,4,5,6-tetrabromophthalimide)
- Test material form:
- solid: crystalline
- Details on test material:
- light yellow crystalline powder with purity of 99%; identified via IR spectrum
Constituent 1
- Radiolabelling:
- no
Sampling and analysis
- Details on sampling:
- 2 fish at each of the 2 dose levels were analyzed at 2, 4, 6 and 8 weeks of exposure. Water samples collected at the same time points for analysis.
Test solutions
- Vehicle:
- yes
- Details on preparation of test solutions, spiked fish food or sediment:
- Test compound (1.0 g) and crystallized sugar (10 g) were ground together. The dispersant HCO-40 (20 g) was added to the mixture and mixted thoroughly. Ion exchanged water was added and filled to 1 L.
Test organisms
- Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- Avg body weight = 21.1 g; avg body length 9.1 cm; lipid content = 4.8%
Study design
- Route of exposure:
- aqueous
- Test type:
- flow-through
- Water / sediment media type:
- natural water: freshwater
- Total exposure / uptake duration:
- 8 wk
- Total depuration duration:
- 0 wk
Test conditions
- Test temperature:
- 21 +/- 1 degrees C
- Dissolved oxygen:
- ~6.5 ppm over the duration of the test
- Nominal and measured concentrations:
- Nominal = 2.0 and 0.2 ppm
Measured = 2.09 (2 Wk), 2.05 (4 Wk), 2.05 (6 Wk) and 2.07 (8 Wk)
= 0.194 (2 Wk), 0.197 (4 Wk), 0.198 (6 Wk), 0.199 (8 Wk)
Results and discussion
Lipid content
- Lipid content:
- 4.8 %
- Time point:
- start of exposure
Bioaccumulation factoropen allclose all
- Type:
- BCF
- Value:
- < 3.3 dimensionless
- Basis:
- whole body w.w.
- Calculation basis:
- other: at 2, 4, 6, and 8 Wk exposure
- Remarks on result:
- other: n=2 fish/wk
- Remarks:
- Conc.in environment / dose:.0.2 ppm
- Type:
- BCF
- Value:
- < 0.3 dimensionless
- Basis:
- whole body w.w.
- Calculation basis:
- other: 2 and 4 Wk
- Remarks on result:
- other: n=2 fish/wk
- Remarks:
- Conc.in environment / dose:2.0 ppm
- Type:
- BCF
- Value:
- < 0.3 dimensionless
- Basis:
- whole body w.w.
- Calculation basis:
- other: 6 Wks
- Remarks on result:
- other: value in 1 of 2 fish at this timepoint
- Remarks:
- Conc.in environment / dose:2.0 ppm
- Type:
- BCF
- Value:
- 0.4 dimensionless
- Basis:
- whole body w.w.
- Calculation basis:
- other: 6 wks
- Remarks on result:
- other: value in 1 of 2 fish at this timepoint
- Remarks:
- Conc.in environment / dose:2.0 ppm
- Type:
- BCF
- Value:
- < 0.3 dimensionless
- Basis:
- whole body w.w.
- Calculation basis:
- other: 8 Wks
- Remarks on result:
- other: value in 1 of 2 fish at this timepoint
- Remarks:
- Conc.in environment / dose:2.0 ppm
- Type:
- BCF
- Value:
- 1.3 dimensionless
- Basis:
- whole body w.w.
- Calculation basis:
- other: 8 Wks
- Remarks on result:
- other: value in 1 of 2 fish at this timepoint
- Remarks:
- Conc.in environment / dose:2.0 ppm
Any other information on results incl. tables
Fish appeared normal throughout the exposure. No mortality.
Detection limit = 0.05 ppm (S/N=2)
% recovery from fish = 77.0%.
Mean water concentration for 8 weeks = 2.07 ppm at the 2.0 ppm nominal level, and 0.199 ppm for the 0.2 ppm level.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- EBTBP did not bioconcentrate in fish exposed to the substance in water over an 8 week period. The study was conducted according to Japanese guidelines in effect at the time of the study's performance. A dispersant was used.
- Executive summary:
EBTBP did not bioconcentrate in fish(Cyprnus carpio)when tested over an 8-week period. The bioconcentration factor (BCF) was < 3.3 at a water concentration of 0.2 ppm (w/v) and 1.3 at a water concentration of 2.0 ppm (w/v) after an 8-week exposure period with a continuous flow system. A semi static dose range-finding study conducted in Orange-red killifish (Oryzias latipes) for this study provided a 48 hr LC50 of > 500 ppm (w/v). This study was conducted according to Japanese MITI guidelines and Good Laboratory Practices guidelines.
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