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EC number: 297-701-9 | CAS number: 93686-48-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- April - May 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Report date:
- 2012
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Phosphorous acid, tri-C12-14-alkyl esters
- EC Number:
- 297-701-9
- EC Name:
- Phosphorous acid, tri-C12-14-alkyl esters
- Cas Number:
- 93686-48-7
- Molecular formula:
- C36H75O3P C38H7903P C40H83O3P C42H87O3P
- IUPAC Name:
- Phosphorous acid, tri-C12-14-alkyl esters
- Test material form:
- other: liquid
- Details on test material:
- Batch no 80627X065A
Constituent 1
Method
- Target gene:
- Genes involved in the functional capability of the bacteria to synthesize histidine
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: TA 1535, TA 97a, TA 98, TA 100 and TA 102
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix
- Test concentrations with justification for top dose:
- 5000 / 1500 / 500 / 150 / 50 µg/plate for the first experiment (plate incorporation method) and 5002 / 2501 / 1251 / 626 / 313 µg/plate (for the pre incubation method)
- Vehicle / solvent:
- tetrahydrofurane (THF) was chosen as vehicle, because the test item was completely soluble, and addition of 5% of this solvent doesn’t have any effects on the viability of the bacteria or the number of spontaneous revertants
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- benzo(a)pyrene
- other: 2-Amino-anthracene and 4-Nitro-1,2-phenylene diamine
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation) for the first experiment, then preincubation method for the second one.
DURATION:
- Preincubation period: incubation over night at 37 °C before the cultures were used in the experiment
- Exposure duration: 48h
SELECTION AGENT (mutation assays): histidine
NUMBER OF REPLICATIONS: four
DETERMINATION OF CYTOTOXICITY:
- Method: background lawn visible or not and the number of revertant colonies reduced or not - Evaluation criteria:
- A test item is considered to have mutagenic potential, if a significant, reproducible in-crease of revertant colonies per plate (increase factor 2) in at least one strain can be observed. A concentration-related increase over the range tested can also be taken as a sign of mutagenic activity
Results and discussion
Test results
- Key result
- Species / strain:
- S. typhimurium, other: TA 1535, TA 97a, TA 98, TA 100 and TA 102
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results:
Negative with and without metabolic activation
The test item Phosphorous acid, tri-C12-14-alkyl esters was stated as not mutagenic under the test conditions. - Executive summary:
Phosphorous acid, tri-C12-14-alkyl esters was tested in vitro in an Ames test with Salmonella typhimurium.
Five concentrations of the test item, dissolved in tetrahydrofurane (THF) (ranging from 5000 to 50 µg/plate) were used in a first plate incorporation method, then five concentrations (ranging from 5001 to 313 µg/plate) were tested in a second experiment via pre incubation method.
Five genetically manipulated strains of Salmonella typhimurium (TA 97a, TA 98, TA 100, TA 102 and TA 1535) were exposed to the test item both in the presence and in the absence of a metabolic activation system (S9) for 48 hours.
None of the concentrations in the two experiments caused a significant increase in the number of revertant colonies in the tested strains.
No signs of toxicity towards the bacteria could be observed.
The sterility control and the determination of the titre didn’t show any inconsistencies. The determined values for the spontaneous revertants of the negative controls were in the normal range. All positive controls showed mutagenic effects with and without metabolic activation.
The study was then considered as valid and the test item didn’t show mutagenic effects towards Salmonella typhimurium, strains TA 97a, TA 98, TA 100, TA 102 and TA 1535.
Therefore, no concentration-effect relationship could be determined.
The test item Phosphorous acid, tri-C12-14-alkyl esters is considered as “not mutagenic under the conditions of the test”.
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