Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 235-468-7 | CAS number: 12237-62-6 This substance is identified in the Colour Index by Colour Index Constitution Number, C.I. 42535:3.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Hydrolysis:
On the basis of the experimental studies of the test chemical and applying the weight of evidence approach, it is concluded that test chemical is not hydrolysable due to lack of fuctional group.
Biodegradation in water:
28-days biodegradation test following the EU Method C.5 (Degradation: Biochemical Oxygen Demand) was performed to determine the ready biodegradability of the test chemical . Bacteria was used as a test inoculum for the study. Bacteria was collected from activated sludge of the sewage treatment plant of CH-4106 Therwil on 16/10/89 The preparation was carried out according to the method described in the guideline. Aniline (20 mg//l) was used as a reference substance for the study. 1200 ml of the mineral solution with the inoculum were aerated for 24 hours in the test vessel. In 300 ml mineral solution 0.5 ml Nonylphenol 10EO5PO (solution of 30 mg in 100 ml bidist. water) and 16.3 rsp. 47.1 mg of test substance were added and homogenized. This solution was given to the test vessel which was immediately connected to the CO2 traps. The CO2 formed by biodegradation was absorbed with NaOH and determined on a carbon analyzer. Due to the poor solubility of the test substance in water, an emulsifier was used to achieve a better distribution in the medium. Determination of the initial CO2 of the 0.05 N sodium hydroxide and the CO2, absorbed in the absorbers filled with 200 ml 0.05 N sodium hydroxide was carried out on the days 3, 7, 10, 13, 17, 20, 24, 27 and 28, respectively. The biodegradation was calculated on the basis of the theoretical carbon content of the test substance and the cumulative quantities of carbon dioxide determined on the days of measurements. The percentage degradation of test substance was determined to be 6.0 and 4.0% (using 10.9 and 30.4 mg/l conc. of test substance) by using CO2 evolution parameter in 28 days. Thus, based on percentage degradation, test chemical is considered to be not readily biodegradable in nature.
Biodegradation in water and sediment:
Estimation Programs Interface prediction model was run to predict the half-life in water and sediment for the test chemical. If released in to the environment, 1.7 % of the chemical will partition into water according to the Mackay fugacity model level III and the half-life period of test chemical in water is estimated to be 180 days (7320 hrs). The half-life ( 180 days estimated by EPI suite) indicates that the chemical is persistent in water and the exposure risk to aquatic animals is high whereas the half-life period of test chemical in sediment is estimated to be 1620.833 days (38900 hrs). Based on this half-life value, it indicates that test chemical is persistent in sediment.
Biodegradation in soil:
The half-life period of test chemical in soil was estimated using Level III Fugacity Model by EPI Suite version 4.1 estimation database. If released into the environment, 67.2 % of the chemical will partition into soil according to the Mackay fugacity model level III. The half-life period of test chemical in soil is estimated to be 360 days (8640 hrs). Based on this half-life value of test chemical, it is concluded that the chemical is persistent in the soil environment and the exposure risk to soil dwelling animals is high.
Bioaccumulation: aquatic / sediment
On the basis of the experimental studies of the test chemical and applying the weight of evidence approach, the BCF value of the test chemical can be expected to be in the range 66 to 500 dimensionless, respectively. Thus, as this value does not exceed the bioconcentration threshold of 2000 indicating that the test chemical is not expected to bioaccumulate in the food chain
Adsorption / desorption
The adsorption coefficient Koc in soil and in sewage sludge of test chemical was determined by the Reverse Phase High Performance Liquid Chromatographic method according to OECD Guideline No. 121 for testing of Chemicals (Experimental study report, 2019). The solutions of the test substance and reference substances were prepared in appropriate solvents. The sample of test chemical was prepared by adding 10 mg in acetonitrile to prepare 1000 mg/L test solution, sonicated for 1 mins and filtered through 0.22 μm nylon syringe filter. pH of the resulting solution was 7.4. The analysis was performed at constant recommended conditions such as temperature 25°C (solvent: water) ratio 55:45 at the flow rate of 1 mL/min at 210 nm. The reference substances were chosen according to estimated Koc range of the test substance and generalize calibration graph was prepared. Selected reference substances and test chemical were prepared in acetonitrile with concentration of 400 mg/L. Both were analyzed on HPLC considering the similar conditions. After analyzing on above conditions calibration curve of selected reference substances was prepared to obtain log Koc value. The reference substances were p-Toluamide, Aniline, N-methylaniline, 4-Chloroaniline, phenanthrene, Naphthalene, DDT, having Koc value ranging from 1.8 to 2.9. The Log Koc value was test chemical was determined to be 2.787±0.001 at 25°C. Thus, based on the result it is concluded that the test chemical has a moderate sorption to soil and sediment and therefore have slow migration potential to ground water.
Additional information
Hydrolysis:
Data available for the test chemical has been reviewed to determine the half-life of hydrolysis as a function of pH fromauthoritative database (HSDB). The results of studies are mentioned below:
In first study it was mentined that Aromatic amines are generally resistant to aqueous environmental hydrolysis therefore,test chemical is not expected to hydrolyze in water.
In another study the test chemical is not expected to undergo hydrolysis in the environment due to the lack of functional groups that hydrolyze under environmental conditions. Therefore it can be concluded that test chemical is not hydrolysable.
On the basis of the experimental studies of the test chemical and applying the weight of evidence approach, it is concluded that test chemical is not hydrolysable due to lack of fuctional group.
Biodegradation in water:
28-days biodegradation test following the EU Method C.5 (Degradation: Biochemical Oxygen Demand) was performed to determine the ready biodegradability of the test chemical . Bacteria was used as a test inoculum for the study. Bacteria was collected from activated sludge of the sewage treatment plant of CH-4106 Therwil on 16/10/89 The preparation was carried out according to the method described in the guideline. Aniline (20 mg//l) was used as a reference substance for the study. 1200 ml of the mineral solution with the inoculum were aerated for 24 hours in the test vessel. In 300 ml mineral solution 0.5 ml Nonylphenol 10EO5PO (solution of 30 mg in 100 ml bidist. water) and 16.3 rsp. 47.1 mg of test substance were added and homogenized. This solution was given to the test vessel which was immediately connected to the CO2 traps. The CO2 formed by biodegradation was absorbed with NaOH and determined on a carbon analyzer. Due to the poor solubility of the test substance in water, an emulsifier was used to achieve a better distribution in the medium. Determination of the initial CO2 of the 0.05 N sodium hydroxide and the CO2, absorbed in the absorbers filled with 200 ml 0.05 N sodium hydroxide was carried out on the days 3, 7, 10, 13, 17, 20, 24, 27 and 28, respectively. The biodegradation was calculated on the basis of the theoretical carbon content of the test substance and the cumulative quantities of carbon dioxide determined on the days of measurements. The percentage degradation of test substance was determined to be 6.0 and 4.0% (using 10.9 and 30.4 mg/l conc. of test substance) by using CO2 evolution parameter in 28 days. Thus, based on percentage degradation, test chemical is considered to be not readily biodegradable in nature.
Biodegradation in water and sediment:
Estimation Programs Interface prediction model was run to predict the half-life in water and sediment for the test chemical. If released in to the environment, 1.7 % of the chemical will partition into water according to the Mackay fugacity model level III and the half-life period of test chemical in water is estimated to be 180 days (7320 hrs). The half-life ( 180 days estimated by EPI suite) indicates that the chemical is persistent in water and the exposure risk to aquatic animals is high whereas the half-life period of test chemical in sediment is estimated to be 1620.833 days (38900 hrs). Based on this half-life value, it indicates that test chemical is persistent in sediment.
Biodegradation in soil:
The half-life period of test chemical in soil was estimated using Level III Fugacity Model by EPI Suite version 4.1 estimation database. If released into the environment, 67.2 % of the chemical will partition into soil according to the Mackay fugacity model level III. The half-life period of test chemical in soil is estimated to be 360 days (8640 hrs). Based on this half-life value of test chemical, it is concluded that the chemical is persistent in the soil environment and the exposure risk to soil dwelling animals is high.
Bioaccumulation: aquatic / sediment
Data available of its read across chemicals has been reviewed to determine the bioaccumulation potential of test chemical. The studies are as mentioned below:
Bioaccumulation test was conducted for estimating the bioconcentration factor (BCF) of test chemical.The bioconcentration factor was determined to be 66 dimesionless, from an estimated log Pow of 3.28 and a regression derived equation.
Another bioaccumulation test was conducted for estimating the bioconcentration factor (BCF) of test chemical.The bioconcentration factor was determined to be 500 dimesionless, from an estimated log Pow of 8.69 and a regression derived equation.
On the basis of the experimental studies of the test chemical and applying the weight of evidence approach, the BCF value of the test chemical can be expected to be in the range 66 to 500 dimensionless, respectively. Thus, as this value does not exceed the bioconcentration threshold of 2000 indicating that the test chemical is not expected to bioaccumulate in the food chain
Adsorption / desorption
The adsorption coefficient Koc in soil and in sewage sludge of test chemical was determined by the Reverse Phase High Performance Liquid Chromatographic method according to OECD Guideline No. 121 for testing of Chemicals (Experimental study report, 2019). The solutions of the test substance and reference substances were prepared in appropriate solvents. The sample of test chemical was prepared by adding 10 mg in acetonitrile to prepare 1000 mg/L test solution, sonicated for 1 mins and filtered through 0.22 μm nylon syringe filter. pH of the resulting solution was 7.4. The analysis was performed at constant recommended conditions such as temperature 25°C (solvent: water) ratio 55:45 at the flow rate of 1 mL/min at 210 nm. The reference substances were chosen according to estimated Koc range of the test substance and generalize calibration graph was prepared. Selected reference substances and test chemical were prepared in acetonitrile with concentration of 400 mg/L. Both were analyzed on HPLC considering the similar conditions. After analyzing on above conditions calibration curve of selected reference substances was prepared to obtain log Koc value. The reference substances were p-Toluamide, Aniline, N-methylaniline, 4-Chloroaniline, phenanthrene, Naphthalene, DDT, having Koc value ranging from 1.8 to 2.9. The Log Koc value was test chemical was determined to be 2.787±0.001 at 25°C. Thus, based on the result it is concluded that the test chemical has a moderate sorption to soil and sediment and therefore have slow migration potential to ground water.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.