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EC number: 236-004-6 | CAS number: 13092-66-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2006
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Justification for type of information:
- REPORTING FORMAT FOR THE ANALOGUE APPROACH
See read-across justification report under Section 13 ‘Assessment Reports’.
1. HYPOTHESIS FOR THE ANALOGUE APPROACH
In accordance with REACH Annex XI, Section 1.5, of Regulation (EC) No. 1907/2006 (REACH) the standard testing regime may be adapted in cases where a grouping or read-across approach has been applied.
The similarities may be based on:
(1) a common functional group
(2) the common precursors and/or the likelihood of common breakdown products via physical or biological processes, which result in structurally similar chemicals; or
(3) a constant pattern in the changing of the potency of the properties across the category
1. Both substances are inorganic salts of a monovalent cation from Group 2 of the periodic table, calcium or magnesium, and phosphoric acid. Thus, they all share the Ca2+ or Mg2+ cation and the PO43- anion as common functional groups.
2. Both substances will ultimately dissociate into the common breakdown products of the Ca2+ or Mg2+ cations and the PO43- anion.
3. Calcium and magnesium orthophosphates have been shown to have a similar toxicological profile and physicochemical nature and therefore this data is considered to be adequate and reliable for use in read-across. Any further testing would not be scientifically justified as all substances will dissociate to their anionic and cationic forms in natural waters and these ions (Ca2+, Mg2+ and PO43-) are all ubiquitous and are not considered to pose a risk of ecotoxicity.
2. SOURCE AND TARGET CHEMICAL(S) (INCLUDING INFORMATION ON PURITY AND IMPURITIES)
See read-across justification report under Section 13 ‘Assessment Reports’.
3. ANALOGUE APPROACH JUSTIFICATION
See read-across justification report under Section 13 ‘Assessment Reports’.
4. DATA MATRIX
See read-across justification report under Section 13 ‘Assessment Reports’. - Reason / purpose for cross-reference:
- read-across: supporting information
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: control and 100 mg/L. Samples were taken after 1, 24, 48, 72, and 96 hours.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 518 mg of the test substance was directly dissolved in 5,000 mL of dilution water and homogenized to prepare a test solution of 100 mg/L. The control used dilution water only. - Test organisms (species):
- Oryzias latipes
- Details on test organisms:
- TEST ORGANISM
- Common name: Japanese rice fish
- Source: The Ecotoxicology Research Team, Korea Institute of Toxicology (KIT), KRICT, Deajeon, Korea (origin: Laboratory of Freshwater Fish stocks, Bioscience Center, Nagoya University, Japan)
- Age at study initiation (mean and range, SD): 3-4 month
- Length at study initiation (length definition, mean, range and SD): 2.8 cm (S.D. = 0.1 cm)
- Weight at study initiation (mean and range, SD): 0.18 g (S.D. = 0.02g)
- Feeding during test: none
ACCLIMATION
- Acclimation period: 7 days
- Acclimation conditions: same as in test
- Feeding frequency: not fed 24 hours prior to test - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 41.7 mg/L as CaCO3
- Test temperature:
- 23.7 ± 0.2 °C
- pH:
- 7.13 - 7.95
- Dissolved oxygen:
- 5.38 - 8.3 mg/L
- Nominal and measured concentrations:
- Nominal test substance concentration: 100 mg/L
The concentrations of the test substance were measured to be 0.53-2.14 mg/L at 100 mg/L (nominal) - Details on test conditions:
- TEST SYSTEM
- Aeration:none
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Filtered tap water (1 µm, membrane filter), residual chlorine or organic substances were removed by filtering through an activated carbon filter.
- Chlorine: < 0.01 mg/L
OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and abnormality after 1, 24, 48, 72, and 96 hours
- Light intensity: 780-800 lux
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 0.1, 1, 10, and 100 mg/L
- Results used to determine the conditions for the definitive study: no mortality observed at 100 mg/L - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 2.14 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 2.14 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- There were no mortalities and adverse effects observed in any of the fish exposed to control and 100 mg/L treatment groups during the test. All results are expressed in terms of nominal concentration as the concept of loading rate.
- Reported statistics and error estimates:
- No statistics performed
- Validity criteria fulfilled:
- not specified
- Conclusions:
- There were no mortalities and adverse effects observed in any of the fish exposed to control and 100 mg/L treatment groups during the test.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2006
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study, data originate in part from a secondary source
- Justification for type of information:
- REPORTING FORMAT FOR THE ANALOGUE APPROACH
See read-across justification report under Section 13 ‘Assessment Reports’.
1. HYPOTHESIS FOR THE ANALOGUE APPROACH
In accordance with REACH Annex XI, Section 1.5, of Regulation (EC) No. 1907/2006 (REACH) the standard testing regime may be adapted in cases where a grouping or read-across approach has been applied.
The similarities may be based on:
(1) a common functional group
(2) the common precursors and/or the likelihood of common breakdown products via physical or biological processes, which result in structurally similar chemicals; or
(3) a constant pattern in the changing of the potency of the properties across the category
1. Both substances are inorganic salts of a monovalent cation from Group 2 of the periodic table, calcium or magnesium, and phosphoric acid. Thus, they all share the Ca2+ or Mg2+ cation and the PO43- anion as common functional groups.
2. Both substances will ultimately dissociate into the common breakdown products of the Ca2+ or Mg2+ cations and the PO43- anion.
3. Calcium and magnesium orthophosphates have been shown to have a similar toxicological profile and physicochemical nature and therefore this data is considered to be adequate and reliable for use in read-across. Any further testing would not be scientifically justified as all substances will dissociate to their anionic and cationic forms in natural waters and these ions (Ca2+, Mg2+ and PO43-) are all ubiquitous and are not considered to pose a risk of ecotoxicity.
2. SOURCE AND TARGET CHEMICAL(S) (INCLUDING INFORMATION ON PURITY AND IMPURITIES)
See read-across justification report under Section 13 ‘Assessment Reports’.
3. ANALOGUE APPROACH JUSTIFICATION
See read-across justification report under Section 13 ‘Assessment Reports’.
4. DATA MATRIX
See read-across justification report under Section 13 ‘Assessment Reports’. - Reason / purpose for cross-reference:
- read-across: supporting information
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: control and 100 mg/L. Samples were taken after 1, 24, 48, 72, and 96 hours.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Tap water was passed through a membrane filter (1 μm) to remove particulate matter and then a high-grade activated carbon filter to remove any organic contaminants. Preliminary solubility trials indicated that this test substance did not produce test solution of 100 mg/L using organic solvents such as acetone, dimethyl sulfoxide (DMSO), ethanol, and N,N-dimethylformamide (DMF). Therefore, the test solution was prepared by direct addition of the test substance to dilution water. - Test organisms (species):
- Oryzias latipes
- Details on test organisms:
- TEST ORGANISM
- Common name: Japanese rice fish
Source: Korea Institute of Toxicology (KIT), KRICT, Deajeon, Korea
- Age at study initiation (mean and range, SD): 3-4 month
- Length at study initiation (length definition, mean, range and SD): 2.0±1.0 cm
- Weight at study initiation (mean and range, SD): 0.19 g (S.D. = 0.03 g)
- Feeding during test: none
ACCLIMATION
- Acclimation period: 7 days
- Acclimation conditions: same as in test
- Feeding frequency: not fed 24 hours prior to test - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 10-250 mg/L CaCO3
- Test temperature:
- 23.3 ± 0.1 °C
- pH:
- 7.18-7.97
- Dissolved oxygen:
- 5.2-8.5 mg/L
- Salinity:
- <0.01 mg/L (residual chlorine)
- Nominal and measured concentrations:
- Nominal test substance concentration: 100 mg/L
The concentrations of the test substance were measured to be 13.0-14.2 mg/L at a nominal concentration of 100 mg/L (13.0-14.2% of nominal concentration) at 0, 48, and 96 hours. - Details on test conditions:
- TEST SYSTEM
- Aeration:none
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Filtered tap water (1 µm, membrane filter), residual chlorine or organic substances were removed by filtering through an activated carbon filter.
- Chlorine: < 0.01 mg/L
OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime
- Light intensity: 990-1060 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and abnormality after 1, 24, 48, 72, and 96 hours
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 0.1, 1, 10, and 100 mg/L
- Results used to determine the conditions for the definitive study: no mortality observed at 100 mg/L - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 13.5 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 13.5 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- There were no mortalities and adverse effects observed in any of the fish exposed to control and 13.5 mg/L treatment effect group during the test. All results were expressed in terms of the mean measured concentration.
- Reported statistics and error estimates:
- No statistics performed
- Sublethal observations / clinical signs:
Table 1. Cumulative mortality of Oryzias latipes
Nominal concentration (mg/L)
Mean measured concentration (mg/L)
Number of organisms tested
Cumulative number of dead fish
24-hour
48-hour
72-hour
96-hour
Control
ND
7
0
0
0
0
100
13.5
7
0
0
0
0
* ND : Not detected
Table 2. Symptoms of intoxication of the test substance to Oryzias latipes
Nominal concentration (mg/L)
Mean measured concentration (mg/L)
Symptoms of Intoxication
24-hour
48-hour
72-hour
96-hour
Control
ND
NOR(7)
NOR(7)
NOR(7)
NOR(7)
100
13.5
NOR(7)
NOR(7)
NOR(7)
NOR(7)
* ND : Not detected
* ( ) : Number of fish
* NOR : Normal
Referenceopen allclose all
Description of key information
Two key studies are submitted to assess the short-term toxicity to fish of magnesium bis(dihydrogenorthophosphate, this study is conducted on two analogous substances (see justification below). On this basis calcium and magnesium orthophosphates are not considered to be toxic to fish. In addition, a number of literature papers investigating the bioavailability of phosphorus and calcium from calcium bis(dihydrogenorthophosphate) in fish are included in section 6.6, 'Additional ecotoxicological information' . Although these studies are not toxicity studies they do support the conclusions that inorganic phosphates are well tolerated by fish and is therefore unlikely to be toxic. As such, no further testing is justified.
The additional supporting data provided were not considered to be adequate or reliable to fulfil neither the guideline nor the regulatory requirement and there is no evidence within this data to suggest that essential validity criteria are met.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
Data on the acute toxicity of magnesium bis(dihydrogenorthophosphate) (CAS 13092 -66 -5) to fish are not available. The assessment was therefore based on a study conducted with the analogue substances calcium hydrogen orthophosphate (CAS 7757 -93 -9) tricalcium bis(orthophosphate) (CAS 7758-87-4) as part of a read across approach, which is in accordance with Regulation (EC) No. 1907/2006, Annex XI, 1.5.
The acute toxicity of calcium hydrogen orthophosphate and tricalcium bis(orthophosphate) to fish was investigated in a study following OECD guideline 203 (Kim et al. 2013).
Calcium hydrogen orthophosphate:
The acute toxicity of calcium hydrogen orthophosphate to fish was investigated in a study following OECD guideline 203 (Kim et al. 2013). The test was set up as a limit test under static conditions. Fish of the species Oryzias latipes were exposed to a nominal test substance concentration of 100 mg/L. The analytical recovery rate of the test substance was 13.0-14.2% of the nominal concentration. During the test period of 96 hours mortality and abnormal behavior of the test organisms were determined after 1, 24, 48, 72, and 96 hours. Mortality or adverse effects of the test substance were not observed. Based on the mean measured concentration an LC50(96 h) > 13.5 mg/L (nominal: LC50(96 h) >100 mg/L) was determined.
Tricalcium bis(orthophosphate):
The test was set up as a limit test under static conditions. Fish of the species Oryzias latipes were exposed to a nominal test substance concentration of 100 mg/L. The analytical recovery rate of the test substance was 0.53-2.14 mg/L at 100 mg/L nominal concentration. During the test period of 96 hours mortality and abnormal behavior of the test organisms were examined after 1, 24, 48, 72, and 96 hours. Mortality or adverse effects of the test substance were not observed. Based on the mean measured concentration a LC50(96 h) > 2.14 mg/L (nominal: LC50(96 h) >100 mg/L) was determined.
Based on the study results for the read across substances calcium hydrogen orthophosphate and tricalcium bis(orthophosphate) the assessed substance trimagnesium bis(orthophosphate) is considered not acutely toxic to fish.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.