Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 249-044-4 | CAS number: 28472-97-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 20 Dec 1993
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- non-GLP
- Qualifier:
- according to guideline
- Guideline:
- DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test solution was prepared according to the national standard procedure (DEV S4): 20 g of the test substance was added to deionised water and shacked overhead for 24 h. This solution was filtered with a folded filter. The filtrate was used for the test. It was added directly to the test vessels and diluted with deionised water to the desired concentration. - Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- - Laboratory culture: A preculture was incubated for 7 h before test initiation at 21 °C.
- Preparation of inoculum for exposure: The preculture was diluted with sterile growth medium to obtain a turbidity of 50 TE/F436. 10 mL was inoculated to 50 mL eluate of the test substance and 10 mL of growth medium. This mixture was filled up with deionised water to a total volume of 100 mL. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 16 h
- Remarks on exposure duration:
- ± 1h
- Test temperature:
- 21 ± 1 °C
- Nominal and measured concentrations:
- Nominal concentration: 10000 mg/L filtered test solution
- Details on test conditions:
- TEST SYSTEM
- Test vessel: conical flasks
- size, fill volume: 300 mL, 100 mL
- Aeration: incubated on a shaker (Braun Certomat R)
- No. of vessels per concentration: 3
- No. of vessels per control: 3
EFFECT PARAMETERS MEASURED: Growth inhibition was measured after 16 h test duration.
TEST CONCENTRATIONS
- Justification for using less concentrations than requested by guideline: Because the test substance is poorly water soluble the filtrate from a stock solution of 10 g/L was used to analyse if there are any effects in the range of water solubility. - Reference substance (positive control):
- no
- Duration:
- 16 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 10 g/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: filtered test solution
- Basis for effect:
- growth inhibition
- Duration:
- 16 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 g/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: filtered test solution
- Basis for effect:
- growth inhibition
Reference
No effects of the test substance in the range of water solubility were observed testing a saturated test solution which was subsequently filtered. Thus, an LC50 (16h) greater then the highest tested concentration of 10 g/L is determined.
Description of key information
EC10 (16 h) > 10000 mg/L (nominal) for growth rate of P. putida (DIN 38412, part 8).
Key value for chemical safety assessment
Additional information
The toxicity of Diisodecyl azelate (CAS 28472-97-1) to Pseudomonas putida was investigated in one study (Institut für gewerbliche Wasserwitschaft und Luftreinhaltung e.V., 1993). The test substance is described as an unknown, variable composition, complex reaction product or biological material (UVCB) under REACH. The study was performed according to DIN guideline 38412, part 8 “Pseudomonas-Cell Growth Inhibition Test”. A single test concentration of 10 g/L was chosen to evaluate the growth inhibition after 16 h. No growth inhibition of P. putida was reported, the EC10 thus beeing greater than 10 g/L. In addition, the toxicity control of an OECD 301B test showed no inhibition at a test substance concentration of 13 mg/L (Roulstone, 2014; please see technical dossier chapter 5.2.1 for further details). Therefore disturbances in the biodegradation process of sewage treatment plants are not anticipated for Diisodecyl azelate.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.