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Diss Factsheets
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EC number: 617-252-8 | CAS number: 81653-77-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- (2S,4S)-4-(phenylsulfanyl)pyrrolidine-2-carboxylic acid
- EC Number:
- 617-252-8
- Cas Number:
- 81653-77-2
- Molecular formula:
- C11H13NO2S
- IUPAC Name:
- (2S,4S)-4-(phenylsulfanyl)pyrrolidine-2-carboxylic acid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Details on test solutions:
- Five different concentrations in a geometric series, namely 10.0, 17.8, 31.6, 56.2 and 100.0 mg/L.
The test was performed in 50 mi glass beakers filled with 40 mi of test medium.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- The study was performed on thè freshwater crustacean Daphnia magna Straus.
At the start of the test, organisms used in the test were 6-24 hours old and were not first brood progeny.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- water hardness = 250 mg/l
- Test temperature:
- The incubation temperature was continuously monitored during the course of the study by the means of a data logger. The measured values ranged between 20.6 and 22.5 °C during the test period, only slightly upper than the OECD Guideline recommended range (20.0 ± 2.0 °C).
- pH:
- The pH of the test medium after preparation was in the range 7.50 – 7.59, the pH of control was 7.36.
- Nominal and measured concentrations:
- 10.0, 17.8, 31.6, 56.2 and 100.0 mg/L - nominal
- Details on test conditions:
- Due the light sensitivity of test item, the test was performed in dark conditions.
A negative control with reconstituted water only was also tested.
For each test concentration and for the control, 20 daphnids were used divided into four replicates of five daphnids each. The daphnids were randomly distributed to thè test vessels
at initiation of thè test. The daphnid s loading rate was more than one per 2 mi of test
solution. The test vessels were replaced in thè dark place to avoid thè penetration of thè light
in thè test vessels. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- IC50
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Remarks on result:
- other:
- Remarks:
- 95% Confidence range 98.6-101.3
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 56.2 mg/L
- Nominal / measured:
- nominal
- Duration:
- 24 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Duration:
- 48 h
- Dose descriptor:
- IC50
- Effect conc.:
- 70 mg/L
- Nominal / measured:
- nominal
- Remarks on result:
- other:
- Remarks:
- 95% confidence range 55.7-85.3
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 17.8 mg/L
- Nominal / measured:
- nominal
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 31.6 mg/L
- Nominal / measured:
- nominal
- Details on results:
- No remarkabie obsen/ations were made conceming thè appearance of the test medium. The test medium was a clear solution throughout the whole test duration.
In the negative control, 0% immobilization was observed during thè test period at each observation time. - Results with reference substance (positive control):
- 24 h IC50 (mg/L) = 1.22
Confidence interval (95%, mg/L) = 0.75-1.77
Reference data (UNI EN ISO 6341/99) = 0.6-2.1 - Reported statistics and error estimates:
- The results were analyzed for significant differences from control with thè Fisher s Exact test (a = 0.1) by thè means of CETIS elaboration software V1.026D.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on nominal concentrations:
24 h NOEC: 56.2 mg/L
24 h LOEC: 100.0 mg/L
24h IC50: 100.0 mg/L
48 h NOEC: 17.8 mg/L
48 h LOEC: 31.6 mg/L
48 h IC50: 70.0 mg/L - Executive summary:
The acute toxicity of test item was tested on Daphnia magna under static conditions, according to the OECD guideline 202 (2004).
The NOEC, LOEC and IC50 values after 24 and 48 h, caiculated based on nominal concentrations were as follows:
24 h NOEC: 56.2 mg/L
24 h LOEC: 100.0 mg/L
24h IC50: 100.0 mg/L
48 h NOEC: 17.8 mg/L
48 h LOEC: 31.6 mg/L
48 h IC50: 70.0 mg/L
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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