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EC number: 226-603-0 | CAS number: 5435-64-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2009-01-22 until 2009-02-06
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Remarks:
- Study conducted to GLP and in compliance with agreed protocolls, with minor deviations from standard guidelines and/or minor methodological deficiencies which does not effect the quality of the results
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 3,5,5-trimethylhexanal
- EC Number:
- 226-603-0
- EC Name:
- 3,5,5-trimethylhexanal
- Cas Number:
- 5435-64-3
- Molecular formula:
- C9H18O
- IUPAC Name:
- 3,5,5-trimethylhexanal
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Test substance was added in an amount above water solubility to the test water as described under Details on test solution
Test solutions
- Vehicle:
- no
- Details on test solutions:
- A stock solution was prepared by weighed out 100 mg/L and was shaken at 20 rpm for 24h at room temperature. After a separation of at least 1h the saturated solution was taken from the homogenous liquid phase. The saturated soluition were diluted 1:1 - 1:2 - 1:4 - 1:8 - 1:16.
Test concentrations were calculated from DOC analysis with the carbon content of 71.6%
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna STRAUS (Clone 5)
- Strain/clone: Straus (Clone 5)
- Source: Institut für Wasser-, Boden- und Lufthygiene (WaBoLu)TEST ORGANISM
- Age: 2 to 24 h old daphnids were used for the test. They were obtained by removing the mother animal twice within 22 h.
- Feeding during test: no
ACCLIMATION
- Acclimation period: at least 2 h in dilution water
- Acclimation conditions (same as test or not): in glass vessels with 1.8 L culture medium, 20 °C +/- 2 °C, 16 h light (20 µE/m2*s)
- Type and amount of food: unicellular green algae with a cell density of >10^6 cells/mL, ad libitum
- Feeding frequency: at least 5 times per week
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- Test medium (nominal) 0.24 mmol CaMg/l
- Test temperature:
- 18 - 22 °C, +-1 °C constant
- pH:
- 7.41 - 7.65
- Dissolved oxygen:
- 7.93 - 8.19 mg/l
- Salinity:
- fresh water conditions
- Nominal and measured concentrations:
- A saturated solution was prepared by weighing out 100 mg/lwhich was shaken with 20 rpm for 24h at room temperature. After a separation phase of at least 1 h the saturated solution was taken from the homogenous liquid phase. Dilutions were tested. 1:1 - 1:2 - 1:4 - 1.8 - 1:16 ,nominal 100 - 50 - 25 - 12.5 - 6.25% of the saturated solution, concentrations verified by DOC analysis according to DIN EN 1484 at 0h and 48h.
Based on the mean measured DOC, the test item concentration were calculated to be 71.2 mg/L, 35.6 mg/L, 17.3 mg/L, 8.66 mg/L, and 4.61 mg/L, respectively - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers (4 cm ID x 7 cm H), 50 ml capacity
- Test volume: 20 mL
- Renewal rate of test solution (frequency/flow rate): no
- No. of organisms per vessel: 5 animals per test concentration and control
- No. of vessels per concentration (replicates): 4 samples
- No. of vessels per control (replicates): 4 samples
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
- Total organic carbon:
- Culture medium different from test medium: Elendt M4 medium
- Intervals of water quality measurement: prior to the test start, the water quality parameter of the dilution water were measured (pH values, dissolved oxygen concentration, conductivity, total hardness, and temperature). The water quality parameter were also measured in the fresh media (0 h) and in the old media (48 h).
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light, 8 h dark cycle
- Light intensity: 20 µE/m2*s
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobility
RANGE-FINDING STUDY
- Test concentrations: saturated solution 1:1 to 1:100 mg/L.
- Results used to determine the conditions for the definitive study: 100 % in the 1:1 dilution level. No immobilization at 1:10, 1:100 and in the control. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate p.a. (Sigma)
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 10.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: CL 9.65 - 10.6
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- 9 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other:
- Remarks:
- concentration based on DOC measurement.
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 8.66 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other:
- Remarks:
- concentration based on DOC measurement
- Details on results:
- The saturated solution and all test dilutions were visually clear. The test item concentrations were calculated from the mean measured DOC concentrations taking the carbon content of 71.6 % into account.
In this study 3,5,5-trimethylhexanal was found to be toxic to Daphnia magna after 48h at concentrations of 17.3 mg/l (LOEC) and higher. The EC50 (48 h) was calculated to be 10.1 mg/L. The NOEC (48 h) is 8.66 mg/l. All effect values are based on the calculated concentration of mean measured DOC concentration.
EC10 (24/48h) 14.0 / 9.00 mg/l
EC50 (24/48h) 15.5/10.1 mg/l , 95% CL 15.1-16.0/9.65-10.6
EC100 (24/48h) 35.6/17.3 mg/l
NOEC (48h) 8.66 mg/l
LOEC (48h) 17.3 mg/l - Results with reference substance (positive control):
- EC50: 1.53 mg/l (CL 1.43 - 1.65 mg/l)
In the prescribed range of 0.6 to 2.1 mg/L according to guideline. - Reported statistics and error estimates:
- no
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- No immobilized dahpnids in the control. Dissolved O2 concentration > 3mg/L.
- Conclusions:
- In this study 3,5,5-trimethylhexanal was found to be toxic to Daphnia magna after 48h at concentrations of 17.3 mg/l (LOEC) and higher. The NOEC is 8.66 mg/l and the EC50 was calculated with 10.1 mg/l. All values are based on DOC analysis.
- Executive summary:
EC50 (48h) is calculated with 10.1 mg/l (CL 95% 9.65 - 10-6 mg/l) based on nominal concentrations.
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