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EC number: 210-848-5 | CAS number: 624-48-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable, publication/study report which meets basic scientific principles, but details missing
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 982
- Report date:
- 1982
- Reference Type:
- secondary source
- Title:
- Maleic acid dimethylester: evaluation of dermal toxicity and genotoxicity
- Author:
- Heimann KG, Jung R, Kieczka H
- Year:
- 1 991
- Bibliographic source:
- Fd Chem Toxic Vol 29 No 8 pp 575-578
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- Deviations:
- not specified
- GLP compliance:
- yes
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- Dimethyl maleate
- EC Number:
- 210-848-5
- EC Name:
- Dimethyl maleate
- Cas Number:
- 624-48-6
- Molecular formula:
- C6H8O4
- IUPAC Name:
- dimethyl (Z)-but-2-enedioate
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- NMRI
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Age at study initiation: 12 weeks
- Weight at study initiation: 27-38 g
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- maize oil
- Details on exposure:
- PREPARATION OF DOSING SOLUTIONS:
- prepared in 10 mL maize oil/kg body weight - Frequency of treatment:
- once
- Post exposure period:
- 24, 48 and 72 hrs
Doses / concentrations
- Remarks:
- Doses / Concentrations:
1000 mg/kg body weight
Basis:
actual ingested
- No. of animals per sex per dose:
- 5
- Control animals:
- yes, concurrent vehicle
- Positive control(s):
- - cyclophosphamide
- Route of administration: oral (gavage)
- Doses / concentrations: 60 mg/kg bw
Examinations
- Tissues and cell types examined:
- bone marrow
- Evaluation criteria:
- 1000 polychromatic erythrocytes per animal were examined for micronuclei
normochromatic erythrocyte swer also counted
Results and discussion
Test results
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- yes
- Vehicle controls validity:
- not specified
- Negative controls validity:
- not specified
- Positive controls validity:
- not specified
Any other information on results incl. tables
Slight cytotoxicity was observed (shift in relation normochromatic/polychromatic erythrocytes).
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative
The results demonstrated that the test substance did not have a clastogenic potential in mice. - Executive summary:
The test substance was dissolved in maize oil and was adminsitered orally to 5 male and 5 female NMRI mice per group. The dose was 1000 mg/kg body weight, the dose volume was 10 ml/kg bw. Negative (vehicle) and positive (cyclophosphamide, 60 mg/kg bw) control groups were included in the study design. Bone marrow was prepared 24, 48 and 72 hours after administration.
No increase in the percentage of polychromatic micronucleated erythrocytes was found and it was concluded that the test susbtance had no clastogenic potential in mice. A shift in the relation of normochromatic to polychromatic erythrocytes indicating slight cytotoxicity proved the bioavailability of the test substance.
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