2,6,6-trimethylbicyclo[3.1.1]heptane-3-carbaldehyde

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: non-GLP study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1986

Report date:

1986

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

GLP compliance:

no

Test type:

other: inhalation hazard test

Test material

Test material information

Constituent 1

Reference substance name:

2,6,6-trimethylbicyclo[3.1.1]heptane-3-carbaldehyde

EC Number:

262-062-7

EC Name:

2,6,6-trimethylbicyclo[3.1.1]heptane-3-carbaldehyde

Cas Number:

60113-43-1

Molecular formula:

C11H18O

IUPAC Name:

2,6,6-trimethylbicyclo[3.1.1]heptane-3-carbaldehyde

Details on test material:

Name of the test substance used in the study report: Formylpinan R

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

The rats were hould in wire mesh cages. They were identified via color labelling on the tail.
Mean body weights: males 276 g, females 205 g
Age of the animals at the start of the study: 8 weeks
The rats were housed in fully air-conditioned rooms with a temperature in the range of 20 - 24°C and a relative humidity in the range of 30 - 70%. The day/night rhythm was 12 h dark and 12 h light. A standardized animal laboratory diet and drinking water were available ad libitum.
Adaption period: at least 5 days

Administration / exposure

Route of administration:

inhalation

Vehicle:

other: unchanged

Details on inhalation exposure:

Generation of the atmosphere for inhalation: exposure
The product was filled to a height of 5 cm into a fritted glass flask (glass filter, pore-size 90 - 150 um, diameter 30 mm), and the weight was determined. The fritted glass flask containing the product was placed in a waterbath maintained at 20°C by a thermostat, and a stream of 200 l/h compressed air was introduced. The mixture of air and test substance generated in this way was passed through a glass distributor to 6 glass tubes in which 3 male and 3 female animals had been placed. The emerging mixtures of test substance and air were exhausted. Suitable measures were applied to ensure that the air supply had the same temperature as in the laboratories, so that the temperature in the exposure apparatus was between 19 and 25°C . There were no deviations from these set conditions which might have adversely affected the results of the study. After 30 minutes the fritted glass flask was replaced by a new one containing fresh test substance as described above. This generator was then used for the remainder of the test.
Determination of the nominal concentration:
The amount of test substance used was determined by reweighing the fritted glass flasks. Exposure lasted for 7 hours. The aim was to determine the exposure time survived by all animals, including an observation period of 14 days.

Duration of exposure:

7 h

No. of animals per sex per dose:

3

Control animals:

no

Details on study design:

Clinical examinations took place each work day. Lethality was checked each day. The animals which died, and those sacrificed with carbon dioxide at the end of the observation period, were subjected to a gross-pathological examination.

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LC0

Effect level:

other: saturated vapour

Exp. duration:

7 h

Remarks on result:

other: no mortality

Effect levels 2

Sex:

male/female

Dose descriptor:

LC0

Effect level:

0.14 mg/L air (nominal)

Exp. duration:

7 h

Mortality:

none

Clinical signs:

other: Accelerated, in some animals intermittent, respiration; slight attempts to escape; wiping of snouts, trembling in some animals.

Gross pathology:

Nothing abnormal found in the organs.

Any other information on results incl. tables

The mean concentration of the test substance, calculated for a test lasting 7 hours was 0.14 mg/l.