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EC number: 479-540-8 | CAS number: 61007-89-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2002-12-11 to 2003-02-04
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: OECD study, GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- -
- EC Number:
- 479-540-8
- EC Name:
- -
- Cas Number:
- 61007-89-4
- Molecular formula:
- C27 H27 Mn N4 O3
- IUPAC Name:
- manganese(3+) 2-[({2-[bis(2-{[(2-oxidophenyl)methylidene]amino}ethyl)amino]ethyl}imino)methyl]benzen-1-olate
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: Samples for analytical determinations were taken from all test concentrations at test start, at 24 hours (old and new test solutions) and at the end of the test (48 hours).
- Sample storage conditions before analysis: -18°C to -25°C
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: As the test item is prone to hydrolysis (please refer to section 5.1.2), the stock solution was prepared as close as practically possible to the start of the test. These treatments fulfill the recommendations of the OECD for poorly soluble substances subject to hydrolysis (OECD, 2000). To maintain the test concentrations constant during exposure, the test was performed under semi-static conditions. The stock solutions was renewed at 24 hours of the test. Each stock solution was prepared as follows: 200.3 mg test item (or 199.9 mg at 24 hours of the test) was weighed, dissolved in 2000 mL test medium. The stock solution was homogenised by ultrasonic treatment for about 10 minutes and stirred for about 5 minutes. Due to the poor solubility of the test item, the not dissolved parts of the test item were separated by filtration (0.45 µm). The filtration was carried out under pressure with a flow rate not greater than 100 mL/min. The filter was preconditioned with 1000 mL of the stock solution. Afterwards, the rest of the stock solution was filtered and the filtrate taken to prepare the test concentrations. Additionally, due to the light sensitivity of the test item, the glass vessel were covered with aluminium foil and the filtration process took place in the dark.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna Straus
- Source: laboratories of the test facility
- Age at study initiation: less than 24 hours
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- ca. 280 mg/L CaCO3
- Test temperature:
- 20.1 - 21.0°C
- pH:
- 8.0 to 8.1.
- Dissolved oxygen:
- 96% and 100%
- Nominal and measured concentrations:
- 4.3, 9.4, 21, 45 and 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL beakers containing 100 mL test medium
- Renewal rate of test solution (frequency/flow rate): 24 hours
- No. of organisms per vessel: 20 Daphnia per concentration
- No. of vessels per concentration: two beakers per test concentration
TEST MEDIUM / WATER PARAMETERS
- in accordance to the guideline
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light daily, 8 hours dark
TEST CONCENTRATIONS
- Spacing factor for test concentrations: ca. 2.2 - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 9.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Mortality of control: no
- Since the test item is prone to hydrolysis (please refer to section 5.1.2), no suitable method was available to quantify the test item in water. Hence all the biological results are related to the nominal concentrations of the test item. - Results with reference substance (positive control):
- The quality of the Daphnia is checked at least every 3 months by determining the EC50 value (24 h) for the reference item potassium dichromate.
Reference control on December 04, 2002: EC50 = 0.9 mg/L
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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