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EC number: 425-660-0 | CAS number: 165101-57-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 2005-06-21 to 2005-06-23
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- updated 13 April 2004
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- adopted 31 July 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- The concentration and stability of the test material in the test preparations were verified by chemical analysis at 0 and 48 hours. A Strata X solid phase extraction (SPE) cartridge was sequentially pre-conditioned with acetonitrile and water. A volume of test sample was eluted through the cartridge. The test material was eluted from the cartridge with acetonitrile and prepared for subsequent GC-FID analysis.
- Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Source: in-house laboratory cultures
- Age at study initiation: approximately 24 h
ACCLIMATISATION
- Type and amount of food: suspension of algae (Chlorella sp.)
- Feeding frequency: daily
Adult Daphnia were maintained in polypropylene vessels containing approximately 2 litres of reconstituted water in a temperature controlled room at approximately 20 °C. The lighting cycle was controlled to give a 16 hours light and 8 hours darkness cycle with 20 minutes dawn and dusk transition periods. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- no
- Hardness:
- The reconstituted water had an approximate theoretical total hardness of 250 mg/L as CaCO3.
- Test temperature:
- 21.1 - 21.6 °C
- pH:
- Control: 7.9
1.0 mg/L: 7.8 - 7.9
1.8 mg/L: 7.8 - 7.9
3.2 mg/L: 7.8 - 7.9
5.6 mg/L: 7.8 - 8.0
10 mg/L: 7.9 - 8.1
18 mg/L: 7.9 - 8.7
32 mg/L: 8.0 - 8.5
56 mg/L: 8.2 - 8.8
100 mg/L: 8.5 - 9.2 - Dissolved oxygen:
- Control: 96 -100 %ASV
1.0 mg/L: 94 -100 %ASV
1.8 mg/L: 93 -100 %ASV
3.2 mg/L: 94 -99 %ASV
5.6 mg/L: 96 -100 %ASV
10 mg/L: 96 -100 %ASV
18 mg/L: 96 -100 %ASV
32 mg/L: 96 -98 %ASV
56 mg/L: 97 -99 %ASV
100 mg/L: 97 - 100 %ASV
ASV = Dissolved oxygen concentration expressed as a percentage of Air Saturation Value - Salinity:
- not applicable
- Nominal and measured concentrations:
- Nominal: 1.0, 1.8, 3.2, 5.6, 10, 18, 32, 56, 100 mg/L
Due to the rapid hydrolysis of the test item, steady measured concentrations of the test item were not achivable. The hydrolysis products could not be detected by the analytical method due to possible but unconfirmed adsorption to surfaces. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL glass jars containing approximately 250 mL of test preparation
- No. of organisms per vessel: 10
- No. of vessels per concentration: 2 (dupliclate test vessels)
- No. of vessels per control (replicates): 2 (dupliclate test vessels)
TEST MEDIUM
- Source/preparation of dilution water: reconstituted water
OTHER TEST CONDITIONS
- Adjustment of pH: The reconstituted water had a pH of 7.8 ± 0.2 adjusted (if necessary) with NaOH or HCl
- Photoperiod: 16 hours light and 8 hours darkness for a period of 48 hours with 20 minute dawn and dusk transition periods
EFFECT PARAMETERS MEASURED :
Mobility
TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations in range finding study: 0.10, 1.0, 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: Yes, the test concentrations to be used in the definitive test were determined by the preliminary range-finding test - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate (0.32, 0.56, 1.0, 1.8 and 3.2 mg/L)
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 21 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95 % confidence limits: 18 - 25 mg/L
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 9.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95 % confidence limits: 7.8 - 11 mg/L
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- <= 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- <= 3.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- Due to the rapid hydrolysis of the test item, steady measured concentrations of the test item were not achievable. The hydrolysis products could not be detected by the analytical method due to possible but unconfirmed adsorption to surfaces.
- Results with reference substance (positive control):
- The results from the positive control with potassium dichromate were within the normal range for this reference material. The mean 48-hour EC50 value calculated from all positive controls was 0.82 mg/L.
- Reported statistics and error estimates:
- not applicable
- Validity criteria fulfilled:
- yes
- Conclusions:
- The acute toxicity of the test material to the freshwater invertebrate Daphnia magna has been investigated and gave a 48-hour EC50 value of 9.5 mg/L with 95 % confidence limits of 7.8 -11 mg/L. The No Observed Effect Concentration at 48 hours was 3.2 mg/L.
- Executive summary:
A study was performed to assess the acute toxicity of the test material to Daphnia magna, according to OECD Guideline 202 and EU method C.2. Following a preliminary range-finding test, twenty daphnids (2 replicates of 10 animals) were exposed to an aqueous solution of the test material at concentrations of 1.0, 1.8, 3.2, 5.6, 10, 18, 32, 56 and 100 mg/L for 48 hours at a temperature of 21.1 to 21.7 °C under static test conditions. The number of immobilised Daphnia were recorded after 24 and 48 hours.
A positive control conducted approximately every six months used potassium dichromate as the reference material. Daphnia magna was exposed to an aqueous solution of the reference material at concentrations of 0.32, 0.56, 1.0, 1.8 and 3.2 mg/L for 48 hours at a temperature of approximately 20 °C under static test conditions. Immobilisation and any adverse reactions to exposure were recorded after 3, 24 and 48 hours.
Due to the rapid hydrolysis of the test item, steady measured concentrations of the test item were not achievable. The hydrolysis products could not be detected by the analytical method due to possible but unconfirmed adsorption to surfaces.
The 48-hour EC50 obtained for the test material to Daphnia magna based on nominal test concentrations of parent test material was 9.5 mg/L with 95 % confidence limits of 7.8 - 11 mg/L. The No Observed Effect Concentration was 3.2 mg/L.
Reference
Description of key information
The acute toxicity of the test material to the freshwater invertebrate Daphnia magna has been investigated and gave a 48-hour EC50 value of 9.5 mg/L with 95 % confidence limits of 7.8 - 11 mg/L. The No Observed Effect Concentration at 48 hours was 3.2 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 9.5 mg/L
Additional information
A study was performed to assess the acute toxicity of the test material to Daphnia magna, according to OECD Guideline 202 and EU method C.2. Following a preliminary range-finding test, twenty daphnids (2 replicates of 10 animals) were exposed to an aqueous solution of the test material at concentrations of 1.0, 1.8, 3.2, 5.6, 10, 18, 32, 56 and 100 mg/L for 48 hours at a temperature of 21.1 to 21.7 °C under static test conditions. The number of immobilised Daphnia were recorded after 24 and 48 hours.
A positive control conducted approximately every six months used potassium dichromate as the reference material. Daphnia magna was exposed to an aqueous solution of the reference material at concentrations of 0.32, 0.56, 1.0, 1.8 and 3.2 mg/L for 48 hours at a temperature of approximately 20 °C under static test conditions. Immobilisation and any adverse reactions to exposure were recorded after 3, 24 and 48 hours.
The 48-hour EC50 obtained for the test material to Daphnia magna based on nominal test concentrations of parent test material was 9.5 mg/L with 95 % confidence limits of 7.8 - 11 mg/L. The No Observed Effect Concentration was 3.2 mg/L.
Due to the rapid hydrolysis of the test item, steady measured concentrations of the test item were not achievable. The hydrolysis products could not be detected by the analytical method due to possible but unconfirmed adsorption to surfaces.
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