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EC number: 939-714-0 | CAS number: 1474044-77-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Start : 29 March 2012 Completion : 16 May 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study without significant deficiencies
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The test substance was not completely soluble in test medium at the loading rates initially prepared. Therefore, 1-Litre test bottles were filled with 200 ml of test substance mixtures in Milli-RO water (tap water purified by reverse osmosis; Millipore Corp., Bedford, Mass., USA) with initial loading rates of 2.5 times the final loading rate. These mixtures were stirred in closed dark brown bottles for approximately 25 hours. Subsequently, 16 ml synthetic medium, 250 ml sludge and Milli-RO water up to 500 ml were added resulting in the required loading rates (see also paragraph 6.4.1 and 6.6). Optimal contact between the test substance and test organisms was ensured applying continuous aeration and stirring.
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- The sludge was coarsely sieved, washed and diluted with ISO-medium. A small amount of the sludge was weighed and dried overnight at ca. 105°C to determine the amount of suspended solids (3.0 g/l of sludge, as used for the test). The pH was 7.8 on the day of testing. The batch of sludge was used one day after collection; therefore 50 ml of synthetic medium was added per litre of activated sludge at the end of the collection day. The sludge was kept aerated at test temperature until use.
16 ml synthetic medium, 250 ml sludge and Milli-RO water up to 500 ml were added resulting in the required loading rates. - Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 3 h
- Post exposure observation period:
- none
- Hardness:
- CaCl2.2H2O 211.5 mg/l
MgSO4.7H2O 88.8 mg/l
NaHCO3 46.7 mg/l
KCl 4.2 mg/l - Test temperature:
- The temperatures measured in the various vessels ranged between 17.3 and 20.8°C and were except for the deviation of max. 0.7°C as described in paragraph 6.9.1, within the ranges prescribed by the protocol.
- pH:
- The pH in all test series, before addition of sludge was between 4.3 and 7.5. After the 3 hour exposure period the pH was between 4.3 and 8.4.
- Dissolved oxygen:
- The aeration was adjusted in such a way that the dissolved oxygen concentration at the start was above 60-70% saturation (60% of air saturation is > 5 mg/l at 20°C)
- Salinity:
- NA
- Nominal and measured concentrations:
- Nominal loading rates (mg/l) were 0, 100, 180, 320, 560 and 1000.
- Details on test conditions:
- Contact time: 3 hours, during which aeration and stirring took place.
Vessels: All glass bottles/vessels.
Milli-RO / Milli-Q water: Tap-water purified by reverse osmosis (Milli-RO) and subsequently passed over activated carbon and ion-exchange cartridges (Milli-Q) (Millipore Corp., Bedford, Mass., USA).
Synthetic medium (=sewage feed)
16 g peptone
11 g meat extract
3 g urea
0.7 g NaCl
0.4 g CaCl2.2H2O
0.2 g MgSO4.7H2O
2.8 g K2HPO4
Dissolved in Milli-Q water, made up to 1 litre and filtered. The pH was within 7.5 ± 0.5.
Air supply: Clean, oil-free air.
Aeration: The aeration was adjusted in such a way that the dissolved oxygen concentration at the start was above 60-70% saturation (60% of air saturation is > 5 mg/l at 20°C) and to maintain the sludge flocs in suspension. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 560 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Details on results:
No statistically significant inhibition of the respiration rate of the sludge was recorded at or below a loading rate of 100 mg di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA) / litre (Bonferroni t-Test: α=0.05 Toxstat). At higher loading rates the inhibitory effect of di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA) on aerobic waste water (activated sludge) bacteria increased with increasing loading rate, ranging from 26% inhibition at a loading rate of 180 mg/l to 65% at loading rate of 1000 mg/l.- Results with reference substance (positive control):
- The EC50 of 3,5-dichlorophenol was in the accepted range of 2 to 25 mg/l for total respiration (5.3 and 4.9 mg/l).
- Reported statistics and error estimates:
- The EC10 was at a loading rate of 110 mg/l (95% confidence interval: 45 - 280 mg/l).
The EC20 was at a loading rate of 170 mg/l (95% confidence interval: 72 - 400 mg/l).
The EC50 was at a loading rate of 560 mg/l (95% confidence interval: 270 - 1190 mg/l).
The EC80 was above a loading rate of 1000 mg/l. - Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of this present test di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA) was not toxic to waste water (activated sludge) bacteria at or below a loading rate of 100 mg/l (NOEC).
- Executive summary:
The influence of di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA) on the respiration rate of activated sludge was investigated after a contact time of 3 hours.The study procedures described in this report were based onthe OECD guideline No. 209, 2010.
DNNSA is a UVCB substance (purity 100%). The test material was a brown highly viscous liquid. The test substance was not completely soluble in test medium at the loading rates initially prepared.Therefore, 1-Litre test bottles were filled with 200 ml of test substance mixtures in Milli-RO water with initial loading rates of 2.5 times the final loading rate. These mixtures were stirred in closed dark brown bottles for approximately 25 hours. Subsequently, 16 ml synthetic medium, 250 ml sludge and Milli-RO water up to 500 ml were added resulting in the required loading rates. Optimal contact between the test substance and test medium was ensured applying continuous aeration and stirring during the 3-hour exposure period. Thereafter, oxygen consumption was recorded for 9 to 14 minutes.
The final test was performed based on the result of a preceding combined limit/range-finding test. Five loading rates, ranging from 100 to 1000 mg/l, increasing with a factor 1.8 were tested. Five replicates per loading rate and sixreplicates for an untreated control group were tested.
No statistically significant inhibition of the respiration rate of the sludge was recordedat or below a loading rate of 100 mg di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA) /litre(Bonferroni t-Test:α=0.05 Toxstat). At higher loading rates theinhibitory effect of di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA) on aerobic waste water (activated sludge) bacteria increased with increasing loading rate, ranging from 26% inhibition at a loading rate of 180 mg/l to 65% at loading rate of 1000 mg/l.
The batch of activated sludge was checked for sensitivity by testing the reference substance 3,5-dichlorophenol, which showed normal sensitivity.
The study met the acceptability criteria prescribed by the protocol and was considered valid.
In conclusion, di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA) was not toxic to waste water (activated sludge) bacteria at or below a loading rate of 100 mg/l. The effect parameters for the inhibition of the respiration rate are presented below.
Parameter
di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA)
Loading rate (mg/l)
NOEC
100
EC10
110 (45 - 280 mg/l)
EC20
170 (72 - 400 mg/l)
EC50
560 (270 - 1190 mg/l)
EC80
>1000
( ) Between brackets the 95% confidence intervals are given.
Reference
Final Test Results
Flask |
Conc./ Loading rate (mg/l) |
Temp (°C) |
pH before addition of sludge |
pH after 3 h contact time |
Respiration rate (mg O2/l h) |
Respiration rate (mg O2/g h)¹ |
% Inhibition respiration relative to the control (mean value) |
C 1 |
0 |
19.4 |
7.5 |
8.0 |
40 |
27 |
|
C 2 |
0 |
19.7 |
7.5 |
8.0 |
39 |
26 |
|
C 3 |
0 |
19.5 |
7.4 |
7.9 |
44 |
29 |
|
C 4 |
0 |
18.2 |
7.4 |
8.0 |
39 |
26 |
|
C 5 |
0 |
20.2 |
7.4 |
7.9 |
51 |
34 |
|
C 6 |
0 |
19.7 |
7.4 |
7.9 |
44 |
29 |
|
Mean |
0 |
|
|
|
43 |
29 |
|
SD |
0 |
|
|
|
5 |
3 |
|
CV (%) |
0 |
|
|
|
12 |
12 |
|
|
|
|
|
|
|
|
|
R 1 |
5.0 |
19.4 |
7.5 |
8.2 |
25 |
17 |
42 |
R 2 |
12 |
19.5 |
7.5 |
8.2 |
13 |
9 |
70 |
R 3 |
30 |
18.5 |
7.5 |
8.3 |
5 |
3 |
88 |
|
|
|
|
|
|
|
|
T 1a |
100 |
19.4 |
7.1 |
8.0 |
39 |
26 |
9 |
T 1b |
100 |
18.9 |
7.2 |
8.1 |
34 |
23 |
21 |
T 1c |
100 |
19.2 |
7.2 |
8.0 |
36 |
24 |
16 |
T 1d |
100 |
19.0 |
7.1 |
8.0 |
35 |
23 |
18 |
T 1e |
100 |
20.0 |
7.2 |
8.0 |
42 |
28 |
2 |
|
|
|
|
|
|
|
|
T 2a |
180 |
19.4 |
6.9 |
8.0 |
34 |
23 |
21* |
T 2b |
180 |
19.5 |
6.9 |
7.9 |
33 |
22 |
23* |
T 2c |
180 |
18.2 |
6.9 |
8.0 |
28 |
19 |
35* |
T 2d |
180 |
18.4 |
6.9 |
8.0 |
29 |
19 |
32* |
T 2e |
180 |
19.7 |
6.9 |
7.9 |
35 |
23 |
18* |
|
|
|
|
|
|
|
|
T 3a |
320 |
19.2 |
6.6 |
7.9 |
32 |
21 |
25* |
T 3b |
320 |
19.9 |
6.5 |
7.9 |
31 |
21 |
28* |
T 3c |
320 |
20.8 |
6.6 |
7.8 |
36 |
24 |
16* |
T 3d |
320 |
20.0 |
6.5 |
7.8 |
32 |
21 |
25* |
T 3e |
320 |
18.2 |
6.5 |
8.0 |
22 |
15 |
49* |
T 4a |
560 |
18.5 |
5.6 |
8.0 |
23 |
15 |
46* |
T 4b |
560 |
17.9 |
5.4 |
8.1 |
19 |
13 |
56* |
T 4c |
560 |
18.4 |
5.5 |
8.0 |
21 |
14 |
51* |
T 4d |
560 |
18.1 |
5.5 |
8.0 |
20 |
13 |
53* |
T 4e |
560 |
18.0 |
5.6 |
8.0 |
20 |
13 |
53* |
|
|
|
|
|
|
|
|
T 5a |
1000 |
19.3 |
4.3 |
8.0 |
16 |
11 |
63* |
T 5b |
1000 |
17.7 |
4.3 |
8.0 |
15 |
10 |
65* |
T 5c |
1000 |
17.3 |
4.3 |
8.0 |
12 |
8 |
72* |
T 5d |
1000 |
18.6 |
4.3 |
7.9 |
15 |
10 |
65* |
T 5e |
1000 |
18.8 |
4.3 |
7.9 |
16 |
11 |
63* |
C: Control
R: Reference substance, 3,5-dichlorophenol
T: Test substance, di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA)
CV: Coefficient of variation
¹ The amount of suspended solids in the final test mixture was 1.5 g/l.
* Statistically significant (Bonferroni t-Test:α=0.05 Toxstat
Description of key information
di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA) was not toxic to waste water (activated sludge) bacteria at or below a loading rate of 100 mg/l (NOEC).
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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