Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 242-768-1 | CAS number: 19035-79-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012-05-22 until 2012-05-24
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Version / remarks:
- 2008-05-30
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- adopted 2010-07-22
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- Adequate amounts of the test item were directly weighed into each test flask. 3 replicates of the test item flasks were prepared (nominal 100 mg test item/L) and six controls (tap water, synthetic sewage and inoculum, but without addition of the test item) were tested in parallel. In parallel to the study with the test item, the reference item 3,5-dichlorophenol was tested at the nominal test concentrations of 3.2, 10 and 32 mg/L under otherwise identical test conditions. One test solution with a final volume of 500 mL was tested per treatment in a glass flask (>500 mL). Samples were prepared in about 15-minute intervals.
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- Origin:
Activated sludge microorganisms from a domestic waste water treatment plant were supplied by the municipal sewage treatment plant Bensheim, Germany.
Conditioning:
The activated sludge used for this study was used as collected, but coarse particles were removed by settling for a short period and then the upper layer decanted. During holding prior to use the sludge was fed daily with 50 mL synthetic sewage (see below) per litre and kept aerated at room temperature overnight.
An aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge to its dry weight determined. Based on the sludge dry matter, calculated amounts of wet sludge were suspended in tap water to yield a concentration equivalent to 2.5 g/L on dry weight basis. This level gave a concentration of 1.0 g/L suspended solids in the test medium. The pH of the activated sludge was 7.4 and therefore no adjustment was necessary. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Test temperature:
- 20 - 22 °C during pre-incubation (3 hours)
18 - 23 °C during evaluation period
The water temperature was measured in one control medium at the start and the end of the incubation period. - pH:
- The pH of the activated sludge inoculum was determined to be pH 7.4. The pH was determined at the start and at the end of the incubation period in all test concentrations and controls.
- Dissolved oxygen:
- The oxygen concentrations were determined at the start and at the end of the incubation period in all test concentrations and controls.
- Nominal and measured concentrations:
- Nominal 100 mg test item/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass flasks of approximately 1 litre volume and Karlsruher flasks of 250 mL volume
- Aeration: compressed air (1.026 litre per minute)
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6 controls, 1 replicate. Positive control: 3 controls, 1 replicate (3 different concentrations of 3,5-Dichlorophenol were examined, 3.2, 10 and 32 mg/L)
- Synthetic sewage was used:
16 g peptone
11 g meat extract
3 g urea
0.7 g NaCl
0.4 g CaCl2 · 2H2O
0.2 g MgSO4 · 7H2O
2.8 g K2HPO4
Filled up to 1 L with deionised water. The pH of this solution was 7.1
OTHER TEST CONDITIONS
- Adjustment of pH: no - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- The respiration rates of the activated sludge treated with the test item at a concentrations of 100 mg/L differed by 10.2 % from control. The respiration rate of the activated sludge to potassium hexadecyl hydrogen phosphate at the rate of 100 mg/L was not statistically significant reduced when compared to the control. The NOEC is established above 100 mg/L.
- Results with reference substance (positive control):
- The 3-hour EC50 of the reference item 3,5-Dichlorophenol was determined to be 2.5 mg/L and was thus in the range of 2 to 25 mg/L for the activated sludge batch which was used.
- Reported statistics and error estimates:
- Student t-test for homogenous variances with Bonferroni-Holm Adjustment, α = 0.05
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the study conditions, the determined NOEC was above 100 mg/L
- Executive summary:
The influence of the test item potassium hexadecyl hydrogen phosphate on the activity of activated sludge was evaluated by measuring the respiration rate under defined conditions according to EU Method C.11 and OECD Guideline 209. The respiration rate (oxygen consumption) of an aerobic activated sludge fed with a standard amount of synthetic sewage was measured in the presence of one concentration of the test item, 100 mg/L (limit test) after an incubation period of 3 hours. 6 controls (tap water, synthetic sewage and inoculum, but without addition of the test item) were tested in parallel and a reference item 3,5-dichlorophenol was tested at the nominal test concentrations of 3.2, 10 and 32 mg/L under otherwise identical test conditions.
The respiration rates of the activated sludge treated with the test item at a concentrations of 100 mg/L differed by 10.2 % from control. The respiration rate of the activated sludge to potassium hexadecyl hydrogen phosphate at the rate of 100 mg/L was not statistically significant reduced when compared to the control. The determined NOEC was above 100 mg/L.
The positive control 3,5-Dichlorophenol was tested in the same way as the test item. The 3-hour EC50 was found to be 2.5 mg/L, thus, laying in the range of 2 - 25 mg/L recommended by the test guidelines. The result confirms the suitability of the activated sludge used.
Reference
Description of key information
In accordance with OECD TG 209 the NOEC was determined to be greater than 100 mg/L.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 100 mg/L
Additional information
The influence of the test item potassium hexadecyl hydrogen phosphate on the activity of activated sludge was evaluated by measuring the respiration rate under defined conditions according to EU Method C.11 and OECD Guideline 209. The respiration rate (oxygen consumption) of an aerobic activated sludge fed with a standard amount of synthetic sewage was measured in the presence of one concentration of the test item, 100 mg/L (limit test) after an incubation period of 3 hours. 6 controls (tap water, synthetic sewage and inoculum, but without addition of the test item) were tested in parallel and a reference item 3,5-dichlorophenol was tested at the nominal test concentrations of 3.2, 10 and 32 mg/L under otherwise identical test conditions.
The respiration rates of the activated sludge treated with the test item at a concentrations of 100 mg/L differed by 10.2 % from control. The respiration rate of the activated sludge to potassium hexadecyl hydrogen phosphate at the rate of 100 mg/L was not statistically significant reduced when compared to the control. The determined NOEC was above 100 mg/L.
The positive control 3,5-Dichlorophenol was tested in the same way as the test item. The 3-hour EC50 was found to be 2.5 mg/L, thus, laying in the range of 2 - 25 mg/L recommended by the test guidelines. The result confirms the suitability of the activated sludge used.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.