Cyclohexanaminium, N,N,N-trimethyl-, sulfate (2:1)

Endpoint summary

Additional information

There are no studies available for Trimethylcyclohexylammonium sulfate, but for Trimethylcyclohexylammonium hydroxid, aqueous solution 50 wt.%.

The test substance Trimethylcyclohexylammonium hydroxid, aqueous solution 50 wt.% was tested for its mutagenic potential based on the ability to induce point mutations in selected loci of several bacterial strains, i.e. Salmonella typhimurium and Escherichia coli, in a reverse mutation assay. The test strains are TA 1535, TA 100, TA 1537, TA 98 and E. coli WP2 uvrA. The dose range are 300 µg - 10000 µg/plate in the SPT (Standard plate test) and 33 µg - 6666 µg/plate in the PIT (preincubation test) both with and without metabolic activation (liver S9 mix from induced rats). No precipitation of the test substance was found with and without S9 mix. A bacteriotoxic effect was observed depending on the strain and test conditions from about 3 333 μg/plate onward. A relevant increase in the number of his+ or trp+ revertants was not observed in the standard plate test or in the preincubation test either without S9 mix or after the addition of a metabolizing system. Thus, under the experimental conditions of this study, the test substance Trimethylcyclohexylammonium hydroxid, aqueous solution 50 wt.% is not mutagenic in the Salmonella typhimurium/Escherichia coli reverse mutation assay in the absence and the presence of metabolic activation.

Justification for selection of genetic toxicity endpoint
only available study

Short description of key information:
Ames: negative

Endpoint Conclusion: No adverse effect observed (negative)

Justification for classification or non-classification

Based on the results, the test item is no subject to classification and labelling according to Directive 67/548/EEC (DSD) and Regulation (EC) No 1272/2008 (CLP).