Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 274-395-5 | CAS number: 70209-97-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: screening test, other
- Remarks:
- BOD5
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 15 November 1995 to 27 December 1995
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.5 (Degradation: Biochemical Oxygen Demand)
- Deviations:
- yes
- Remarks:
- Guideline: Aerate over night after addition of the inoculum
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch number of test material:
1.56
- Expiration date of the lot/batch:
June 2000
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: 8 °C (light protected)
- Solubility aof the test substance in the solvent/dispersant/vehicle/test medium: > 1 g/l [in water] - Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Seeding water of a communal sewage treatment plant Pro Rheno Basel collected on November 14, 1995
- Concentration of sludge: 6 to 800 mg/l. - Duration of test (contact time):
- 5 d
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Test temperature: 20 ± 1 °C
- pH: 6.5 - Reference substance:
- other: D(+) - Glucose (Merck Nr. 8337) / L-Glutamic acid (Merck Nr. 291)
- Preliminary study:
- None
- Test performance:
- None
- Remarks on result:
- not measured/tested
- Parameter:
- BOD5
- Value:
- 59 mg O2/g test mat.
- Results with reference substance:
- The BOD5 of Glucose/Glutamic acid solution is 192 mg O2/l
- Validity criteria fulfilled:
- no
- Interpretation of results:
- other: BOD5 has been determined
- Conclusions:
- The BOD5 of FAT 20075/B corrected by the blank control is 59 mg O2/g.
- Executive summary:
The study "Determination of the BOD5" was conducted according to ISO 5815 Second Edition 1989-08-01 (E) corresponding to 92/69/EEC C.5. guideline and in accordance with GLP. The biochemical oxygen demand of FAT 20075/B is determined by measuring the dissolved oxygen concentration before and after 5 days incubation at 20 °C in different test concentrations diluted in water saturated with dissolved oxygen that contains a seed of microorganisms. The test organism was collected from a communal sewage treatment plant Pro Rheno Basel collected on November 14,1995. The test substance was tested in concentrations from about 6 to 800 mg/l. The reference substance is a mixture of 150 mg D(+)-Glucose and 150 mg L-Glutamic acid in 11 aqueous solution. This reference substance is tested in a concentration of 20 ml/l. The BOD5 of FAT 20075/B is 59 mg O2/g while of the reference substance is 192 mg O2/g.
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 08 November 1995 - 21 December 1995
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch number of test material: 1.56
- Expiration date of the lot/batch: June 2000
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: 8°C (light protected)
- Solubility of the test substance in the solvent/dispersant/vehicle/test medium: > 1 g/l [in water] - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- Activated sludge of a communal sewage treatment plant Ara Reinach collected on 7.11.95
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 150 mg/L
- Based on:
- DOC
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- A mixture of a total test volume of 2 litres containing test substance, mineral medium and activated sludge was agitated and aerated at 20 - 25 °C in glass vessels for 28 days. A reference control containing activated sludge, mineral medium and reference substance was running in duplicate under the same test conditions. A blank control containing activated sludge and mineral medium but without test substance was running in parallel under the same test conditions. The biodegradation process was monitored by determination of DOC in centrifuged samples taken at certain intervals. The ratio of eliminated DOC, corrected for the blanc after each time interval, to the initial DOC value was expressed as the percentage biodegradation at the sampling time. The concentration of dissolved oxygen was checked at regular intervals to ensure that the oxygen did not fall below 1 mg/l. The pH was checked and adjusted to pH 6.5 - 8.0 if necessary.
- Reference substance:
- diethylene glycol
- Test performance:
- No data
- Parameter:
- % degradation (DOC removal)
- Value:
- 47
- Sampling time:
- 28 d
- Details on results:
- Test substance:
Adsorption: The adsorption of the test substance FAT 20075/B after 3 h was: 7 %
Biodegradation: The biodegradation of the test substance FAT 20075/B after 28 days was: 47 %
Total Elimination: (regarding the adsorption and the biodegradation)
The total elimination of the test substance FAT 20075/B after 28 days was 51 % (average of 2 tests running in parallel) - Results with reference substance:
- The biodegradation, corrected by the blank control and measured as DOC (mg/l) is: 98 % 8th day (average of 2 tests running in parallel).
- Validity criteria fulfilled:
- no
- Interpretation of results:
- not inherently biodegradable
- Conclusions:
- FAT 20075/B considered as non-biodegradable.
- Executive summary:
The inherent biodegradability of FAT 20075/B was determined in a Zahn-Wellens / EMPA - Test according to the OECD Guideline for Testing of Chemicals, No. 302B, 1992. The test substance FAT 20075/B and the reference substance Diethylenglycol, respectively, were tested in concentrations of 150 mg/l DOC. The adsorption of the test substance FAT 20075/B after 3 hours was 7 %, the biodegradation of the test substance FAT 20075/B after 28 days was 47 % while the total elimination of the test substance FAT 20075/B after 28 days was 51 %. The biodegradation of the reference substance after 8 days was 98 %. The biodegradation of FAT 20075/B after 28 days was 47 % and is considered as non-biodegradable.
- Endpoint:
- biodegradation in water: screening tests
- Remarks:
- Chemical oxygen demand
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 29 November 1995 to 18 December 1995
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.6 (Degradation: Chemical Oxygen Demand)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch number of test material: 1.56
- Expiration date of the lot/batch: June 2000
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: 8 °C (light protected)
- Solubility of the test substance in the solvent/dispersant/vehicle/test medium: > 1 g/l [in water] - Oxygen conditions:
- aerobic
- Inoculum or test system:
- not specified
- Duration of test (contact time):
- 2 h
- Initial conc.:
- 2.5 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- test mat. analysis
- Details on study design:
- A predetermined amount of the test substance FAT 20075/B dissolved in water is oxidized by potassium dichromate in a strong sulphuric acid medium with silver sulphate as a catalyst under reflux for 2 hours. The residual dichromate is determined by titration with standardized ferrous ammonium sulphate.
- Reference substance:
- other: Potassium hydrogen phthalate
- Preliminary study:
- Not applicable
- Test performance:
- Not applicable
- Remarks on result:
- not measured/tested
- Details on results:
- Not applicable
- Parameter:
- COD
- Value:
- 1 167 mg O2/g test mat.
- Results with reference substance:
- The COD of the reference substance is: 197 mg O2/g.
- Validity criteria fulfilled:
- not applicable
- Interpretation of results:
- other: COD has been determined.
- Conclusions:
- The COD of FAT 20075/B is 1167 mg O2/g.
- Executive summary:
The purpose of the study was to determine COD of the test substance according to DIN 38409 - H 41-1 (1980) corresponding to EEC Directive 92 /69 / C.6. (1992) and in accordance with GLP. A predetermined amount of the test substance FAT 20075/B dissolved in water is oxidized by potassium dichromate in a strong sulphuric acid medium with silver sulphate as a catalyst under reflux for 2 hours. The residual dichromate is determined by titration with standardized ferrous ammonium sulphate. The test substance was tested with a sample weight of 2.5 mg. The reference substance was Potassium hydrogen phthalate. It was tested with 20 ml of a 0.17 g/l solution. Based on study, the COD of FAT 20075/B is 1167 mg O2/g while of the reference substance is 197 mg O2/g.
Referenceopen allclose all
Adsorption: Difference between DOC-values of the start and after 3 hours.
Biodegradation: Difference between DOC-values of 3 hours and the end of the test.
Total Elimination: Difference between DOC-values of the start and the end of the test
Description of key information
Acid Red 252 is not biodegradable. In a key study the inherent biodegradability of FAT 20075/B was determined according to the OECD Guideline 302B. The adsorption of the test substance FAT 20075/B after 3 hours was 7 %; the biodegradation of the test substance FAT 20075/B after 28 days was 47 % while the total elimination of the test substance FAT 20075/B after 28 days was 51 %. The biodegradation of the reference substance after 8 days was: 98 %. In supporting studies, BOD5 is 59 mg O2/g determined while COD is, 1167 mg O2/g. Considering the COD value determined with the test substance which is 1167 mg O2/g, the resulting BOD5/COD quotient is 0.05, indicating that the test substance is unlikely to be biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- not biodegradable
- Type of water:
- freshwater
Additional information
By the nature of their design and use, textile dyes are not intended to be readily biodegradable as this would assist in the rapid destruction of the dyestuff, rendering it unfit for purpose. There are ISO, European, American (AATCC) and national standards for the colour fastness of dyes. Dyes are required to have specific fastness properties. If the dyes were biodegradable, it would not be possible for them to have these fastness properties. As such, it is accepted that such substances are not readily biodegradable under relevant environmental conditions. A published study (Pagga & Brown, 1986) describes the results of the testing of 87 dyestuffs in short-term aerobic biodegradation tests. The authors of this publication concluded that dyestuffs are very unlikely to show any significant biodegradation in such tests and that 'there seems little point in carrying out such test procedures’ on dyestuffs.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.