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EC number: 212-603-8 | CAS number: 831-52-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in mammalian cells
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- secondary literature
- Justification for type of information:
- Data is from secondary literature.
Data source
Reference
- Reference Type:
- secondary source
- Title:
- OPINION on test chemical
- Author:
- European Commission
- Year:
- 2 011
- Bibliographic source:
- Directorate-General for Health & Consumers; Scientific Committee on Consumer Safety SCCS
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 476 (In Vitro Mammalian Cell Gene Mutation Test)
- Principles of method if other than guideline:
- To evaluate the mutagenic potential of test chemical in mouse lymphoma L5178Y cells by In mammalian cell gene mutation assay.
- GLP compliance:
- not specified
- Type of assay:
- mammalian cell gene mutation assay
Test material
- Reference substance name:
- Sodium 2-amino-4,6-dinitrophenoxide
- EC Number:
- 212-603-8
- EC Name:
- Sodium 2-amino-4,6-dinitrophenoxide
- Cas Number:
- 831-52-7
- Molecular formula:
- C6H5N3O5.Na
- IUPAC Name:
- sodium 2-amino-4,6-dinitrophenoxide
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- mouse lymphoma L5178Y cells
- Additional strain / cell type characteristics:
- other: tk+/-
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9-mix
- Test concentrations with justification for top dose:
- experiment 1: 112.5, 225, 450, 900, 1350 and 1800 μg/ml with and without S9-mix
experiment 2: 28.1, 56.3, 112.5, 225, 337.5 and 450 μg/ml without S9-mix
112.5, 225, 450, 750, 900, 1050 and 1200 μg/ml with S9-mix - Vehicle / solvent:
- de-ionised water
Controlsopen allclose all
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- cyclophosphamide
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- methylmethanesulfonate
- Details on test system and experimental conditions:
- Treatment experiment 1: 4h treatment with and without S9-mix
experiment 2: 4h treatment with and 24h without S9-mix - Evaluation criteria:
- The cells were evaluated for mutagenic potential.
Results and discussion
Test results
- Species / strain:
- mouse lymphoma L5178Y cells
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Remarks on result:
- other: No mutagenic effect were observed
Applicant's summary and conclusion
- Conclusions:
- Under the experimental conditions reported, the test item did not induce mutations in the mouse lymphoma thymidine kinase locus assay using the cell line L5178Y in the absence and presence of metabolic activation.
- Executive summary:
Test substance was assessed for its possible mutagenic potential. For this purpose In vitro Mammalian cell gene mutation assay was performed as per OECD 476 in Mouse Lymphoma cell line L5178Y. The test material was exposed at the concentration mention below.
experiment 1: 112.5, 225, 450, 900, 1350 and 1800 μg/ml with and without S9-mix
experiment 2: 28.1, 56.3, 112.5, 225, 337.5 and 450 μg/ml without S9-mix
112.5, 225, 450, 750, 900, 1050 and 1200 μg/ml with S9-mix
No substantial and reproducible concentration-dependent increase in mutant colony numbers was observed in both main experiments. No relevant shift of the ratio of small versus large colonies was observed up to the maximal concentration of the test item. Appropriate reference mutagens were used as positive controls and showed a distinct increase in induced mutant colonies, indicating that the tests were sensitive and valid. The concentration range of the main experiments was adjusted to toxicity data and the occurrence of precipitation. Therefore test substance was considered to be non-mutagenic in Mouse Lymphoma cell line L5178Y by In vitro Mammalian cell gene mutation assay. Hence the substance cannot be classified as gene mutant in vitro.
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