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EC number: 466-490-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Experiment start date - 04 August 2006; Experiment completion date - 15 August 2006; Study completion date - 27 October 2006.
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- yes
- Remarks:
- The test method was modified following a method developed by RCC Ltd (Ref. 1) to quantify not only the algicidal effect of colored test substances, but also the growth inhibition effect caused by reduced light intensities in the colored test media.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- yes
- Remarks:
- The test method was modified following a method developed by RCC Ltd (Ref. 1) to quantify not only the algicidal effect of colored test substances, but also the growth inhibition effect caused by reduced light intensities in the colored test media.
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Identity: FAT 40827/A
Batch: T2 5572 BOP 01/06
Purity: determined in this study
Appearance: black sticky powder
Expiration date: 28.02.2011
Storage: at room temperature - Analytical monitoring:
- yes
- Details on sampling:
- The samples were stored deep-frozen and protected from light until analysis was performed. Treatment samples and control samples were thawed at 20 °C (water bath) for 1 hours and shaken manually to obtain homogeneous sample solutions.
- Vehicle:
- no
- Details on test solutions:
- The test medium of the highest nominal test item concentration of 100 mg/L was prepared by dissolving 59.3 mg of the test item completely in 600 mL of test water using intense stirring for 10 minutes at room temperature. The test medium was diluted with test water to prepare the test media with the lower test item concentrations. The test media were prepared just before addition of algae.
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Strain: Strain No. 86.81 SAG
- Source (laboratory, culture collection): Collection of Algal Cultures (SAG, Institute for Plant Physiology, University of Göttingen, D-37073 Göttingen, Germany). - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Remarks on exposure duration:
- 3 days for acclimation and 3 days for the definitive test
- Hardness:
- 0.24 mmol/L (= 24 mg/L as CaC03)
- Test temperature:
- 23 °C
- pH:
- 8.2 - 9.2
- Nominal and measured concentrations:
- Nominal: 4.6, 10, 22, 46 and 100 mg/L
Measured: measured concentrations of the test item in the test media were between 96 to 101 % of the nominal values. - Details on test conditions:
- Test water: synthetic test water:
Macro-nutrients
NaHCO3 50.0 mg/L
CaCl2 × 2 H2O 18.0 mg/L
NH4Cl 15.0 mg/L
MgSO4 × 7 H2O 15.0 mg/L
MgCl2 × 6 H2O 12.0 mg/L
KH2PO4 1.6 mg/L
Trace elements
Na2EDTA × 2 H2O 100.0 μg /L
FeCl3 × 6 H2O 80.0 μg /L
MnCl2 × 4 H2O 415.0 μg /L
H3BO3 185.0 μg /L
Na3MoO4 × 2 H2O 7.0 μg /L
ZnCl2 3.0 μg /L
CoCl2 × 6 H2O 1.5 μg /L
CuCl2 × 2 H2O 0.01 μg /L
Conductivity: <0.1 μS/cm
The test was started by inoculation of a biomass of 10.000 algal cells per ml test medium.
Test vessel: Erlenmeyer flasks (50mL)
Replicate: 3 flasks per concentration and 6 flasks in control
Each Erlenmeyer flask was placed in a black cylinder, coated inside with aluminum foil. The cylinders were covered with glass dishes, the dishes were covered with watch glass dished to prevent evaporation. - Reference substance (positive control):
- no
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 37 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 36-39
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 18 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 18-19
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 5.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 4.7-5.6
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 4.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 22 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- At the higher concentrations of FAT 40827/A of 46 and 100 mg/L, the inhibitory effect on the growth of Scenedesmus subspicatus was caused in a high degree by the indirect effect, the light absorption in the colored test solutions. However, at the lower test item concentrations, the inhibitory effect of the test item in experimental part A was higher than the inhibitory effect caused by the reduced light intensity determined in experimental part B. Thus, a real toxic effect of the test item on the growth of Scenedesmus subspicatus can not be excluded. Therefore, the results of the experimental part A, where the algae grew in the test media with dissolved test item as in a usual algal growth inhibition test were taken into consideration for the determination of the toxic effect of the test item on the growth Scenedesmus subspicatus.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The results of the experimental part A, where the algae grew in the test media with dissolved test item as in a usual algal growth inhibition test were taken into consideration for the determination of the toxic effect of the test item on the growth Scenedesmus subspicatus. The result of Experiment A indicated ErC50 was above 100 mg/L and NOEC was 10 mg/L.
- Executive summary:
The test was performed to determine the acute toxic effects of test substance on the growth of algae and also the growth inhibition effect due to reduced light intensities in the coloured test solution during 72 hours according to the modified OECD TG 201 under GLP conditions. 4.6, 10, 22, 46 and 100 mg/L and a control of test substance were applied in the definitive test. Although no positive reference was performed in the test, for the determination of the LOEC and NOEC the calculated mean biomass and the mean growth rate at the test concentrations were tested for significant differences when compared to the control values by Dunnett-tests. Therefore, the results are still valid. The results of the experimental part A, where the algae grew in the test media with dissolved test item as in a usual algal growth inhibition test were taken into consideration for the determination of the toxic effect of the test item on the growth Scenedesmus subspicatus. The result of Experiment A indicated ErC50 was above 100 mg/L, NOEC was 10 mg/L and LOEC was 22 mg/L. Thus, the substance is not classified as per EU CLP (Regulation (EC) No 1272/2008).
Reference
Description of key information
The ErC50 was above 100 mg/L.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 100 mg/L
- EC10 or NOEC for freshwater algae:
- 10 mg/L
Additional information
The test was performed to determine the acute toxic effects of test substance on the growth of algae and also the growth inhibition effect due to reduced light intensities in the coloured test solution during 72 hours according to the modified OECD TG 201 under GLP conditions. 4.6, 10, 22, 46 and 100 mg/L and a control of test substance were applied in the definitive test. Although no positive reference was performed in the test, for the determination of the LOEC and NOEC the calculated mean biomass and the mean growth rate at the test concentrations were tested for significant differences when compared to the control values by Dunnett-tests. Therefore, the results are still valid. The results of the experimental part A, where the algae grew in the test media with dissolved test item as in a usual algal growth inhibition test were taken into consideration for the determination of the toxic effect of the test item on the growth Scenedesmus subspicatus. The result of Experiment A indicated ErC50 was above 100 mg/L, NOEC was 10 mg/L and LOEC was 22 mg/L. Thus, the substance is not classified as per EU CLP (Regulation (EC) No 1272/2008).
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