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EC number: 806-734-5 | CAS number: 109828-01-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2013-01-28 to 2013-2-28
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: well documented guideline study with certificated GLP compliance
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Ministerium für Arbeit, Integration und Soziales des Landes Nordrhein-Westfalen
Test material
- Reference substance name:
- 66790-57-6
- Cas Number:
- 66790-57-6
- IUPAC Name:
- 66790-57-6
- Test material form:
- liquid: viscous
- Details on test material:
- Name as cited in the study report: Additin E 459
Other name: Triethylene glycol dimaleate
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
At the time of the testing the CAS Number that was used for Triethylene glycol dimaleate was 66790-57-6
In the course of the project the CAS Number 109828-01-5 was assigned, as this number describes better the structure of the main compound
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Control : at 72 hours
Test concentration : at 0 and 72 hours
Test solutions
- Vehicle:
- no
- Details on test solutions:
- 99.8 mg of the test item were added to 1 litre of dilution water, treated for 60 seconds at 8000 rpm with an ultra turrax and afterwards stirred for 24 h on a magnetic stirrer. The pH was measured to be 5.8 and was adjusted to pH 8.3.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- Strain of the test species obtained from 'The Collection of Algal Cultures' of the Institute of Plant Physiology at the University of Göttingen (Germany).
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Post exposure observation period:
- No details available
Test conditions
- Hardness:
- 1.3 °dH, corresponding to 22.5 mg/L CaCO3
- Test temperature:
- 21 - 24°C
- pH:
- 8.1 (start)
9.8 (end) - Dissolved oxygen:
- No details available
- Salinity:
- No details available
- Nominal and measured concentrations:
- 100 mg/L (nominal concentration)
- Details on test conditions:
- Test vessels : 300 mL Erlenmeyer flasks with cotton stoppers, test volume: 100 mL
Culturing apparatus : Shaking incubator in which a temperature in the range 21 °C to 24 °C was maintained at +/- 2 °C, and continuous uniform illumination was provided in the spectral range 400 to 700 nm. Temperature was measured and recorded daily - Reference substance (positive control):
- no
- Remarks:
- not stringent according to OECD 201
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- The cell densities were measured at 24 hour intervals. Inhibition of the algal population was measured as reduction in growth rate (index r), relative to control cultures grown under identical conditions. Growth rates were also used to calculate a No Observed Effect Concentration and a Lowest Observed Effect Concentration according to STUDENT-t test for Homogeneous Variances
- Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- No details available
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- Validity criteria of the EU method C.3 guideline were met
- Conclusions:
- No toxic effects against algae were observed at a limit test concentration of 100 mg/L under exposure conditions.
- Executive summary:
A study was performed to assess the adverse effects of Additin E 459 on the growth rate (= rate of increase in cell density with time) and the yield (= biomass at time t minus initial biomass) of the planktonic freshwater algal species Desmodesmus subspicatus (former name: Scenedesmus subspicatus) over several generations.
The study was conducted in accordance with Commission Regulation (EC) No 761/2009 amending Regulation No 440/2008, Method C.3 ‘Freshwater Alga and Cyanobacteria, Growth inhibition test’ (2009) which is equivalent to OECD Guideline for Testing of Chemicals No. 201 (2006).
Exponentially growing algal cells were exposed for a period of 72 hours to a limit test concentration of nominally 100 mg/L of Additin E 459 dissolved in dilution water. Auxiliaries used to prepare the test media were an ultra turrax and a magnetic stirrer.
The cell densities were measured at 24 hour intervals. Inhibition of the algal population was measured as reduction in growth rate (index r), relative to control cultures grown under identical conditions. Growth rates were also used to calculate a No Observed Effect Concentration and a Lowest Observed Effect Concentration according to STUDENT-t test for Homogeneous Variances. The following values were determined:
Results [mg/L]:
ErC 50* (0-72 h): > 100
ErC 10* (0-72 h): > 100
NOEC [r]* (ta 0.05): = 100
LOEC [r]* (ta 0.05): > 100
No toxic effects against algae were observed at a limit test concentration of 100 mg/L under exposure conditions.
* Reduction of growth rate (ErCx, NOEC [r]) is the preferred endpoint according to OECD 201 and for regulatory purposes in the EU. Results relating to yield (EyCx, NOEC [y]) were calculated to fulfil regulatory requirements in some countries (but not in the EU) and are given in the results section of this report.
The results are expressed in terms of nominal concentrations. Effective concentrations correspond to 104.8 % of nominal values at 0 hours, and to 99.0 % of nominal values at 72 hours.
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