6-amino-5-formamido-1,3-dimethyluracil

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Cross-reference

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

6-amino-5-formamido-1,3-dimethyluracil

EC Number:

231-501-4

EC Name:

6-amino-5-formamido-1,3-dimethyluracil

Cas Number:

7597-60-6

Molecular formula:

C7H10N4O3

IUPAC Name:

N-(6-amino-1,3-dimethyl-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)formamide

Details on test material:

- Name of test material (as cited in study report): 1,3-Dimethyl-4-amino-5-formylaminouracil, roh
- Physical state: Solid
- Analytical purity: 99.4%
- Lot/batch No.: 2799
- Storage condition of test material: Storage at room temperature under nitrogen

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Mannheim, Germany. Collected on 24 September 2010
- Preparation of inoculum for exposure: Inoculum was pre-aerated four days before the start of the test
- Initial cell/biomass concentration: 30 mg/L dry substance

Duration of test (contact time):

28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

ca. 95 mg/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

20 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

TEST CONDITIONS
- Test temperature: 22 ± 2 °C

TEST SYSTEM
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: pre-aeration for four days before test start
- Measuring equipment: carbon dioxide trap
- Test performed in open system: no

SAMPLING
- Sampling frequency: every other day

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: no
- Other: Inhibition control

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

Parameter:

% degradation (CO2 evolution)

Value:

< 10

Sampling time:

28 d

BOD5 / COD results

Results with reference substance:

71% after 14 days

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The test substance was not ready biodegradable under the test conditions within 28 days.

Executive summary:

Materials and methods

A CO2-Evolution Test was conducted according to OECD Guideline 301 B over a period of 28 days.

Results and discussion

No biodegradation of test substance was observed during a 28 d incubation period.. The test item is therefore considered to be poorly biodegradable.