bis(2-ethylhexyl) 4,4’-{6-[4-tert-butylcarbamoyl)anilino]-1,3,5-triazine-2,4-diyldiimino}dibenzoate

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1997

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Report date:

1997

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.3 (Algal Inhibition test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

421-450-8

EC Name:

-

Cas Number:

154702-15-5

Molecular formula:

C44H59N7O5

IUPAC Name:

2-ethylhexyl 4-[(4-{[4-(tert-butylcarbamoyl)phenyl]amino}-6-[(4-{[(2-ethylhexyl)oxy]carbonyl}phenyl)amino]-1,3,5-triazin-2-yl)amino]benzoate

Details on test material:

- Name of test material (as cited in study report): RA 3643
- Substance type: white powder
- Physical state: solid
- Lot/batch No.: 87/96
- Storage condition of test material: refrigerator temperature.
The test substance was stated to be insoluble in water, and its purity was stated to be > 99%

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Details on test solutions:

For the limit test 270 mg was dissolved in 10 ml of DMSO, from which 100 mcl was dispersed in one litre of medium, so as to yield a test substance concentration of 2.7 mg/l

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

The fresh water green alga Selenastrum capricornutum was supplied by the 'American Type Culture Collection', c/o Sales Department, USA.

Study design

Test type:

not specified

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

68 h

Test conditions

pH:

The pH was measured at the start and after 68h in selected cultures.
The pH was found to have remained stable during the test (pH 8.2-8.4)

Reference substance (positive control):

yes

Remarks:

K2Cr2O7 and/or 3.5-dichlorophenol

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

68 h

Dose descriptor:

NOEC

Effect conc.:

>= 2.7 mg/L

Nominal / measured:

nominal

Basis for effect:

growth rate

Effect concentrations 2

Duration:

68 h

Dose descriptor:

NOEC

Effect conc.:

>= 2.7 mg/L

Nominal / measured:

nominal

Basis for effect:

biomass

Effect concentrations 3

Duration:

68 h

Dose descriptor:

EC50

Effect conc.:

> 2.7 mg/L

Nominal / measured:

nominal

Basis for effect:

growth rate

Effect concentrations 4

Duration:

68 h

Dose descriptor:

EC50

Effect conc.:

> 2.7 mg/L

Nominal / measured:

nominal

Basis for effect:

biomass

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

No inhibition of any significance was observed with the RA3643. Of the three parameters involved, the effect on the vialibility of the inoculum was considered less reliable and less relevant than the other parameters.