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EC number: 448-050-6 | CAS number: 444065-11-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 25 Jul - 08 Aug 2003
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP -guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- -
- EC Number:
- 448-050-6
- EC Name:
- -
- Cas Number:
- 444065-11-6
- Molecular formula:
- C41 H57 N5 O8
- IUPAC Name:
- 2,6-di-tert-butyl-4-methylcyclohexyl 5-{[bis(2-ethoxy-2-oxoethyl)carbamoyl]oxy}-2-(4-tert-butylphenyl)-6-cyano-1H-pyrrolo[1,2-b][1,2,4]triazole-7-carboxylate
- Details on test material:
- - Name of test material (as cited in study report): UC-141
- Physical state: White powder
- Analytical purity: 99.7% (area%), 98.2% (mass%)
- Lot/batch No.: CE-203
- Expiration date of the lot/batch: 01 Jan 2005
- Storage condition of test material: At room temperature in the dark
Constituent 1
Method
- Target gene:
- his operon (Salmonella strains)
trp operon (Escherichia coli strain)
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- Cofactor supplemented post-mitochondrial fraction (S9 mix), prepared from the livers of rats treated with Aroclor 1254
- Test concentrations with justification for top dose:
- Range-finding study (results included in first experiment): 3, 10, 33, 100, 333, 1000, 3330 and 5000 μg/plate; TA 100 and WP2 uvr A; with and without metabolic activation
First experiment: 3, 10, 33, 100, 333 and 1000 μg/plate; TA 1535, TA 1537 and TA 98; with and without metabolic activation
Second experiment: 10, 33, 100, 333 and 1000 μg/plate; TA 1535, TA 1537, TA 100 and TA 98, and WP2 uvr A; with and without metabolic activation - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: Dimethyl sulfoxide. The stock solution was treated with ultrasonic waves to obtain a homogeneous suspension. Test substance concentrations were prepared directly prior to use and used within 4 hours after preparation.
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Remarks:
- vehicle control with and without metabolic activation
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: Sodium azide, 9-aminoacridine, daunomycin, methylmethanesulfonate, 4-nitroquinoline N-oxide, 2-aminoanthracene (see 'Any other information on results incl. tables' for details).
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: In agar (plate incorporation)
DURATION
- Exposure duration: 48 hours
NUMBER OF REPLICATIONS: 3 replications each, in 2 independent experiments
DETERMINATION OF CYTOTOXICITY
- Method: Reduction of the bacterial background lawn, increase in the size of the microcolonies and reduction of the revertant colonies
OTHER: In a range-finding study with strain TA 100 and the WP2 uvr A strain, with and without S9-mix, 8 concentrations (3-5000 μg/plate) were tested in triplicate. The dose range finding test results were reported as a part of the first experiment of the mutation assay. The highest concentration of the test substance used in the subsequent mutation assay was the level at which the test substance exhibited limited solubility. - Evaluation criteria:
- a) The negative control data (number of spontaneous revertants per plate) should be within the laboratory background historical range for each tester strain.
b) The positive control chemicals should produce responses in all tester strains which are within the laboratory historical range documented for each positive control substance. Furthermore, the mean plate count should be at least two times the concurrent vehicle control group mean.
c) The selected dose range should include a clearly toxic concentration or should exhibit limited solubility as demonstrated by the preliminary toxicity range-finding test or should extend to 5 mg/plate.
A test substance is considered negative (not mutagenic) in the test if:
a) The total number of revertants in any tester strain at any concentration is not greater than two times the solvent control value, with or without metabolic activation.
b) The negative response should be reproducible in at least one independently repeated experiment.
A test substance is considered positive (mutagenic) in the test if:
a) It induces at least a 2-fold, dose related increase in the number of revertants with respect to the number induced by the solvent control in any of the tester strains, either with or without metabolic activation. However, any mean plate count of less than 20 is considered to be not significant.
b) The positive response should be reproducible in at least one independently repeated experiment.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity, but tested up to precipitating concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity, but tested up to precipitating concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Precipitation: Slight precipitation of the test substance on the plates was observed at the start and at the end of the incubation period at concentrations of 1000 μg/pIate and above (see Table 1 and 2). The precipitate does not influence automated counting of the plate.
RANGE-FINDING/SCREENING STUDIES: The results of the range-finding study was used as the first of two independent experiments. Precipitation of UC-141 on the plates was observed at the start and at the end of the incubation period at 333 μg/plate and above with and without metabolic activation in all strains. No cytotoxicity was observed at any dose level in any strain, with or without metabolic activation.
COMPARISON WITH HISTORICAL CONTROL DATA: Yes (data not shown). The results of the negative and positive control data fell within the historical range,
ADDITIONAL INFORMATION ON CYTOTOXICITY: No cytotoxicity was observed up to and including 5000 μg/plate with and without metabolic activation in TA 100 and WP2 uvr A; and up to and including 1000 μg/plate with and without metabolic activation in TA 1535, TA 1537 and TA 98. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Table 1: Test results of experiment 1, including range-finding study (plate incorporation)
With or without S9-Mix |
Test substance concentration (μg/plate) |
Mean number of revertant colonies per plate (average of 3 plates ± Standard deviation) |
||||
Base-pair substitution type |
Frameshift type |
|||||
TA 100 |
TA1535 |
WP2 uvr A |
TA98 |
TA1537 |
||
– |
Solvent control |
138 ± 13 |
9 ± 2 |
13 ± 3 |
17 ± 1 |
7 ± 3 |
– |
3 |
143 ± 21 |
6 ± 2 |
16 ± 4 |
14 ± 2 |
5 ± 2 |
– |
10 |
147 ± 2 |
7 ± 1 |
14 ± 4 |
13 ± 4 |
6 ± 1 |
– |
33 |
148 ± 15 |
4 ± 1 |
19 ± 3 |
15 ± 2 |
6 ± 1 |
– |
100 |
147 ± 3 |
6 ± 2 |
20 ± 5 |
12 ± 3 |
4 ± 1 |
- |
333 |
150 ± 17 |
6 ± 1 |
17 ± 7 |
16 ± 1 |
3 ±1 |
- |
1000P |
161 ± 8 |
- |
12 ± 4 |
- |
- |
- |
3330P |
167 ± 13 |
- |
19 ± 6 |
- |
- |
– |
5000P |
152 ± 23 |
- |
13 ± 3 |
- |
- |
Positive controls, –S9 |
Name |
MMS |
SA |
4NQO |
DM |
9AA |
Concentrations (μg/plate) |
650 |
1 |
10 |
4 |
60 |
|
Mean No. of colonies/plate (average of 3 ± SD) |
1025 ± 27 |
207 ±8 |
712 ± 6 |
317 ± 69 |
265 ± 24 |
|
+ |
Solvent control |
136 ± 2 |
9 ± 2 |
18 ± 1 |
23 ± 2 |
6 ± 1 |
+ |
3 |
130 ± 18 |
7 ± 1 |
19 ± 2 |
20 ± 2 |
5 ± 2 |
+ |
10 |
135± 9 |
8 ± 2 |
27 ± 5 |
23 ± 3 |
4 ± 1 |
+ |
33 |
149 ± 9 |
7 ± 1 |
20 ± 6 |
24 ± 3 |
5 ± 2 |
+ |
100 |
155 ± 3 |
5 ± 1 |
15 ± 3 |
22 ± 2 |
4 ± 2 |
+ |
333 |
142 ± 15 |
8 ± 2 |
21 ± 2 |
20 ± 3 |
6 ± 1 |
+ |
1000P |
142 ± 11 |
- |
20 ± 3 |
- |
- |
+ |
3330P |
142 ± 27 |
- |
15 ± 1 |
- |
- |
+ |
5000P |
141 ± 17 |
- |
15 ± 3 |
- |
- |
Positive controls, +S9 |
Name |
2AA |
2AA |
2AA |
2AA |
2AA |
Concentrations (μg/plate) |
1 |
1 |
5 |
1 |
2.5 |
|
Mean No. of colonies/plate (average of 3 ± SD) |
825 ± 35 |
153 ± 14 |
90 ± 2 |
529 ± 46 |
279 ± 14 |
SA = sodium azide
DM = daunomycine
MMS = methylmethanesulfonate
4NQO = 4-nitroquinoline-N-oxide
9AA = 9-aminoacridine
2AA = 2-Aminoanthracene
P = Slight precipitate
Table 2: Test results of experiment 2 (plate incorporation)
With or without S9-Mix |
Test substance concentration (μg/plate) |
Mean number of revertant colonies per plate (average of 3 plates ± Standard deviation) |
||||
Base-pair substitution type |
Frameshift type |
|||||
TA 100 |
TA1535 |
WP2 uvr A |
TA98 |
TA1537 |
||
– |
Solvent control |
146 ± 5 |
8 ± 4 |
9 ± 5 |
13 ± 2 |
4 ± 3 |
– |
10 |
131 ± 9 |
9 ± 4 |
8 ± 2 |
13 ± 5 |
7 ± 4 |
– |
33 |
147 ± 13 |
6 ± 2 |
7 ± 2 |
19 ± 4 |
6 ± 3 |
– |
100 |
143 ±13 |
4 ± 1 |
6 ± 2 |
13 ± 3 |
6 ± 2 |
- |
333 |
138 ± 18 |
8 ± 4 |
8 ± 4 |
12 ± 2 |
6 ± 5 |
- |
1000P |
147 ± 2 |
8 ± 3 |
10 ± 1 |
17 ± 3 |
6 ± 2 |
Positive controls, –S9 |
Name |
MMS |
SA |
4NQO |
DM |
9AA |
Concentrations (μg/plate) |
650 |
1 |
10 |
4 |
60 |
|
Mean No. of colonies/plate (average of 3 ± SD) |
808 ± 18 |
794 ± 14 |
751 ± 14 |
661 ± 112 |
454 ± 32 |
|
+ |
Solvent control |
110 ± 9 |
7 ± 3 |
12 ± 5 |
26 ± 9 |
4 ± 1 |
+ |
10 |
115 ± 11 |
10 ± 3 |
9 ± 3 |
15 ± 1 |
7 ± 3 |
+ |
33 |
122 ± 5 |
6 ± 1 |
8 ± 2 |
19 ± 3 |
6 ± 4 |
+ |
100 |
105 ± 13 |
4 ± 3 |
7 ± 1 |
19 ± 5 |
8 ± 3 |
+ |
333 |
116 ± 5 |
10 ± 3 |
8 ± 5 |
18 ± 3 |
6 ± 2 |
|
1000P |
106 ± 14 |
10 ± 3 |
7 ± 1 |
16 ± 4 |
4 ± 2 |
Positive controls, +S9 |
Name |
2AA |
2AA |
2AA |
2AA |
2AA |
Concentrations (μg/plate) |
1 |
1 |
5 |
1 |
2.5 |
|
Mean No. of colonies/plate (average of 3 ± SD) |
719 ± 34 |
117 ± 11 |
142 ± 21 |
302 ± 14 |
257 ± 18 |
SA = sodium azide
DM = daunomycine
MMS = methylmethanesulfonate
4NQO = 4-nitroquinoline-N-oxide
9AA = 9-aminoacridine
2AA = 2-Aminoanthracene
P = Slight precipitateApplicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
CLP: not classified
DSD: not classified
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