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EC number: 214-406-2 | CAS number: 1125-21-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: genome mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1997
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: According to OECD 471, 472 and EEC Directives 92/69, B 13 and 14. Study conducted under GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
- Report date:
- 1997
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 472 (Genetic Toxicology: Escherichia coli, Reverse Mutation Assay)
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2,6,6-trimethylcyclohex-2-ene-1,4-dione
- EC Number:
- 214-406-2
- EC Name:
- 2,6,6-trimethylcyclohex-2-ene-1,4-dione
- Cas Number:
- 1125-21-9
- Molecular formula:
- C9H12O2
- IUPAC Name:
- 2,6,6-trimethylcyclohex-2-ene-1,4-dione
- Reference substance name:
- Oxoisophoron
- IUPAC Name:
- Oxoisophoron
- Details on test material:
- - Name of test substance: Oxoisophoron
- Batch No.: not given
- CAS No.: 1125-21-9
- Date of manufacture: May 18, 1995
- Test substance No.: 96/87
- Degree of purity: 98.87%
- Appearance, consistency: Yellowish liquid
- Storage: Room temperature
Constituent 1
Constituent 2
Method
- Target gene:
- his, trp
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- Male Sprague-Dawley rat liver S-9 fraction. Induction with Aroclor 1254.
- Test concentrations with justification for top dose:
- 20; 100; 500; 2,500 and 5,000 µg/plate
- Vehicle / solvent:
- DMSO
Controls
- Untreated negative controls:
- yes
- Remarks:
- vehicle control and sterility control
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene (2-AA): 2.5 and 60 µg with S-9; N-methyl-N'-nitro-o-phenylendiamine: 5 µg without S-9; 4-nitro-o-phenylendiamine (NOPD): 10 µg without S-9; 9-aminoacridine: 100 µg without S-9; N-ethyl-N'-nitro-N-nitrosoguanidine: 10 µg without S-9.
- Details on test system and experimental conditions:
- Test conducted according to the Ames procedure (standard plate test and preincubation test, with and without S9-Mix).
Number of plates: 3 test plates per dose or per control.
Vehicle: DMSO
The study comprised 2 independent experiments.
1st Experiment:
Strains: TA 1535, TA 100, TA 1537, TA 98, E. coli WP2 uvrA.
Doses: 0; 20; 100; 500; 2500 and 5000 ug/plate.
Vehicle: DMSO.
Type of test and test conditions: standard plate test with and without S9-Mix.
Number of plates: 3 test plates per dose or per control.
2nd Experiment:
Strains: TA 1535, TA 100, TA 1537, TA 98, E. coli WP2 uvrA.
Doses: 0; 20; 100; 500; 2500 and 5000 ug/plate.
Vehicle: DMSO.
Type of test and test condition: preincubation test with and without S9 mix.
Number of plates: 3 test plates per dose or per control. - Evaluation criteria:
- In general, a substance to be characterized as positive in the bacterial tests has to fulfil the following requirements:
- doubling of the spontaneous mutation rate (control)
- dose-response relationship
- reproducibility of the results.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- other: A slight decrease in the nunber of his* revertants was occasionally observed. A reduced his- background growth was found using TA 98 in the preincubation test without S-9 mix at 5,000 µg/plate.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- According to the results of the present study, oxoisophoron is not mutagenic in the Ames test and in the Escherichia coli - reverse mutation assay under the chosen experimental conditions. Thus, no classification is warranted according to Regulation (EC) No. 1272/2008 and EU Directive 67/548/EEC.
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