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EC number: 695-745-7 | CAS number: 1079221-49-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 26-Jul-2010 to 19-Aug-2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study conducted in compliance with international guidelines
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2-({3-aminopyrazolo[1,5-a]pyridin-2-yl}oxy)ethan-1-ol hydrochloride
- EC Number:
- 695-745-7
- Cas Number:
- 1079221-49-0
- Molecular formula:
- C9 H11 N3 O2, ClH
- IUPAC Name:
- 2-({3-aminopyrazolo[1,5-a]pyridin-2-yl}oxy)ethan-1-ol hydrochloride
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
Based on the information provided by the Sponsor, the test item has a limited stability in aqueous solution and thus no stock solution was prepared.
Therefore, test item amounts of 5.36, 16.04, 50.23, 160.49 and 500.71 mg were weighed by means of an analytical balance and transferred to the designated test vessels with 250 mL tap water just before the start of the test. The pH was measured in each test vessel and adjusted to pH 6.0 - 6.1 with a diluted sulfuric acid or sodium hydroxide solution to improve the stability of the test item in aqueous solution.
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- The study was performed with aerobic activated sludge from a wastewater treatment plant (ARA Ergolz II, Füllinsdorf / Switzerland) treating predominantly domestic wastewater. The sludge was washed twice with tap water by centrifugation and the supernatant liquid phase was decanted. A homogenized aliquot of the final sludge suspension was weighed, thereafter dried and the ratio of wet to dry weight was calculated.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Hardness:
- /
- Test temperature:
- The temperature in the test media, measured in one control, was 20 °C at the start and 21 °C at the end of the incubation period.
- pH:
- Each test medium (test item, reference item and control) was adjusted to a pH of 6.0 - 6.2 with a diluted sodium hydroxide or sulfuric acid solution. After pH adjustment, 16 mL synthetic sewage feed was added.
- Dissolved oxygen:
- The concentration of dissolved oxygen did not drop below 2.5 mg/L during the incubation period.
- Salinity:
- /
- Nominal and measured concentrations:
- Nominal concentrations: 10, 32, 100, 320 and 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
Test item amounts of 5.36, 16.04, 50.23, 160.49 and 500.71 mg were weighed by means of an analytical balance and transferred to the designated test vessels with 250 mL tap water just before the start of the test. The pH was measured in each test vessel and adjusted to pH 6.0 - 6.1 with a diluted sulfuric acid or sodium hydroxide solution to improve the stability of the test item in aqueous solution. Immediately thereafter, 16 mL synthetic sewage feed and about 34 mL tap water were added. Finally, at the start of the test 200 mL activated sludge inoculum (adjusted to pH 6.2) was added.
Additionally, two controls, containing only tap water, synthetic sewage feed and activated sludge inoculum, were tested in parallel to the five concentrations of the test item under identical test conditions (for composition, see table on next page). Analog to the test item solution, the pH of 250 mL tap water was adjusted to pH 6.0 - 6.2 with a diluted sulfuric acid. Immediately thereafter, 16 mL synthetic sewage feed were added and the test media filled up to 300 ml with tap water. Finally, 200 mL activated sludge inoculum (adjusted to pH 6.2) was added at the test start.
TEST MEDIUM / WATER PARAMETERS
An aliquot of washed sludge was suspended in tap water to obtain a concentration equivalent to 4 g dry material per liter. During the holding period of three days prior to use, the sludge was fed daily with 50 mL synthetic sewage feed per liter and was kept at room temperature under continuous aeration until use. Before use, the dry weight of the activated sludge was measured again in the inoculum used for the test. The pH of the activated sludge inoculum was adjusted from 7.1 to 6.2 with a diluted sulfuric acid solution.
- Synthetic sewage feed: 16 g peptone, 11 g meat extract, 3 g urea, 0.7 g NaCl, 0.4 g CaCl2 × 2H2O, 0.2 g MgSO4 × 7H2O, 2.8 g K2HPO4 filled up to a final volume of 1 liter with deionized water.
- Intervals of water quality measurement: The pH values and dissolved oxygen concentrations were determined after the addition of synthetic sewage feed and activated sludge in all test media and the controls at the start and at the end of the 3-hour incubation period. The water temperature was measured in one control at the start and at the end of the incubation period. Before the addition of activated sludge and synthetic sewage feed, the appearance of the test media was recorded.
OTHER TEST CONDITIONS
- Adjustment of pH: yes, see above
- Photoperiod: /
- Light intensity: /
- Other: During the incubation period of 3 hours all test media and the controls were continuously aerated by intense stirring on magnetic stirrers to avoid possible foaming and/or stripping of the test item.
EFFECT PARAMETERS MEASURED : For measurement of the respiration rate a well-mixed sample of each test medium was poured into a BOD-flask after three hours incubation time, and was not further aerated. Then the dissolved oxygen concentration was measured with an oxygen electrode (WTW TriOxmatic® 300 and an oxygen meter WTW Oxi 539, Wissenschaftlich-Technische Werkstaetten WTW, Weilheim/Germany), and was continuously recorded. During measurement, the samples were continuously stirred on a magnetic stirrer. The oxygen consumption rate (in mg O2 L-1 minute-1) was determined from the linear part of the respiration curve in the range 6.5–2.5 mg O2/L. In case of very rapid oxygen consumption, the range used was below the limits indicated above but always within the linear part of the respiration curve. In case of low oxygen consumption the rate was determined over a period of at least ten minutes. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 189 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 74 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
Any other information on results incl. tables
Up to and including the concentration of 32 mg/L, the test item had no significant inhibitory effect (≤10%) on the respiration rate of activated sludge after the incubation period of three hours. At the higher test item concentrations of 100, 320 and 1000 mg/L, the inhibitory effects were 12, 87 and 79%, respectively. The oxygen consumption rates of the two controls (run at the start and at the end of the test) differed only by 1% (guideline-recommendation: the two control respiration rates should be within 15% of each other).
The 3-hour EC50 of the reference item 3,5-dichlorophenol (positive control) was calculated to be 14 mg/L (the 95% confidence limits could not be calculated due to mathematical reasons). The 3-hour EC50 is within the guideline-recommended range of 5–30 mg/L, confirming suitability of the activated sludge used.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- The O2 consumption rates of the 2 controls differed by 1%. The concentration of dissolved O2 did not drop below 2.5 mg/L during the incubation period. Just before measurement of the respiration rates the dissolved O2 concentrations were at least 7mg/L.
- Executive summary:
The inhibitory effect of the test item on the respiration rate of aerobic wastewater microorganisms of activated sludge was investigated in a 3-hour respiration inhibition test according to the EU Commission Directive 88/302/EEC, Part C.11, Commission Regulation (EC) No. 440/2008, Part C.11 and the OECD Guideline for Testing of Chemicals, No. 209.
The following nominal concentrations were tested: 10, 32, 100, 320 and 1000 mg/L.
In addition, two controls and three different concentrations of the reference item 3,5-dichlorophenol (5, 16 and 50 mg/L) were tested in parallel. The results of these treatments confirmed suitability of the activated sludge and the method used.
Up to and including the concentration of 32 mg/L, the test item had no significant inhibitory effect (≤10%) on the respiration rate of activated sludge after the incubation period of three hours. At the higher test item concentrations of 100, 320 and 1000 mg/L, the inhibitory effects were 12, 87 and 79%, respectively.
The calculated 3-hour EC values were as follows :
NOEC (determined as the calculated 3-hour EC10): 74 mg/L
EC50: 189 mg/L
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