Bis(2,3-epoxypropyl) terephthalate

Toxicity to aquatic algae and cyanobacteria

Description of key information

Toxicity of bis(2,3-epoxypropyl)terephthalate to Pseudokirchneriella subcapitata in an Algal Growth Inhibition Test according to OECD 201 and Commission Regulation (EC) No 761/2009, C.3.

Key value for chemical safety assessment

EC50 for freshwater algae:

2.94 mg/L

EC10 or NOEC for freshwater algae:

0.327 mg/L

Additional information

The acute Toxicity of bis(2,3-epoxypropyl)terephthalate to the unicellular green algal speciesPseudokirchneriella subcapitata was determined in a static 72 hours test. The test was conducted in accordance with OECD Guideline 201 and the Commission Regulation (EC) No 761/2009. This study encompassed 6 treatment groups (5 dose rates of the test item: A Filtrate of nominal 100 mg test item/L and sequential dilutions of 1:2, 1:4, 1:8 and 1:16; corresponding the geometric mean measured concentrations of 4.23, 2.03, 0.889, 0.327 and 0.162 and a control) with three replicates per test concentration and six replicates for the control. At test start 50 mL of the test concentrations were inoculated with 5000 algal cells per mL test medium and defined volumes of the algal suspensions were sampled after 24, 48 and 72 hours for determination of cell densities by spectrophotometrical measurement. The samples collected at start and after 72 hours were analysed via HPLC method.

The 72-hour E_rC₅₀value was calculated to be 2.94 mg test item/L and the 72-hour E_yC₅₀ was calculated to be 1.13 mg test item/L. The 72-hour NOE_rC was determined to be 0.327 and the associated 72-hour LOE_rC is 0.889 mg test item/L. The 72-hour NOE_yC was determined to be 0.327 and the associated 72-hour LOE_yC is 0.889 mg test item/L